ENSG00000153879

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284000 ENSG00000153879 HEK293_OSMI2_2hA HEK293_TMG_2hB CEBPG protein_coding protein_coding 17.21023 9.457015 26.00148 0.8918787 0.6733473 1.458167 12.816674 5.376909 22.667385 0.1912023 1.4454220 2.073725 0.6808958 0.5826667 0.8700333 0.2873667 0.006207777 0.001851795 FALSE TRUE
MSTRG.16935.4 ENSG00000153879 HEK293_OSMI2_2hA HEK293_TMG_2hB CEBPG protein_coding   17.21023 9.457015 26.00148 0.8918787 0.6733473 1.458167 3.212526 3.896846 1.337767 1.0639383 0.8196085 -1.535434 0.2501708 0.3978333 0.0531000 -0.3447333 0.135701770 0.001851795 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000153879 E001 0.8052696 0.1822217401 5.641970e-01 6.915358e-01 19 33373685 33373696 12 + 0.202 0.318 0.870
ENSG00000153879 E002 2.4024342 0.0060260091 1.603636e-01 2.756242e-01 19 33373697 33373708 12 + 0.575 0.317 -1.358
ENSG00000153879 E003 4.4049386 0.0036762257 8.830860e-02 1.725415e-01 19 33373709 33373717 9 + 0.772 0.499 -1.194
ENSG00000153879 E004 93.5084124 0.0116615169 7.444506e-01 8.327845e-01 19 33373718 33373895 178 + 1.931 1.954 0.078
ENSG00000153879 E005 7.9853599 0.0020418460 5.666710e-01 6.936071e-01 19 33374312 33374613 302 + 0.938 0.873 -0.250
ENSG00000153879 E006 5.7052102 0.0045242981 7.039981e-01 8.026095e-01 19 33378782 33379143 362 + 0.814 0.766 -0.188
ENSG00000153879 E007 605.6569466 0.0002040783 1.678294e-09 2.384847e-08 19 33379144 33380108 965 + 2.721 2.807 0.287
ENSG00000153879 E008 96.8724655 0.0012714615 1.908240e-06 1.490949e-05 19 33380109 33380298 190 + 2.009 1.802 -0.698
ENSG00000153879 E009 364.8878041 0.0013821464 3.050858e-08 3.429310e-07 19 33380299 33381226 928 + 2.565 2.433 -0.440
ENSG00000153879 E010 67.6515135 0.0018562392 6.986243e-02 1.431501e-01 19 33381227 33381244 18 + 1.827 1.739 -0.298
ENSG00000153879 E011 558.5490340 0.0054018237 2.492692e-01 3.850468e-01 19 33381245 33382686 1442 + 2.700 2.742 0.140