ENSG00000153561

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000283632 ENSG00000153561 HEK293_OSMI2_2hA HEK293_TMG_2hB RMND5A protein_coding protein_coding 17.96092 11.2406 24.83506 1.408089 0.935571 1.142957 16.548876 7.310460 24.83506 0.5452927 0.935571 1.762953 0.8780000 0.6594333 1 0.3405667 1.510734e-32 1.510734e-32 FALSE TRUE
ENST00000472843 ENSG00000153561 HEK293_OSMI2_2hA HEK293_TMG_2hB RMND5A protein_coding processed_transcript 17.96092 11.2406 24.83506 1.408089 0.935571 1.142957 1.376379 3.884736 0.00000 1.0073461 0.000000 -8.605382 0.1188667 0.3363000 0 -0.3363000 3.255042e-30 1.510734e-32 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000153561 E001 136.833521 0.0003083460 2.595415e-15 9.303567e-14 2 86720291 86720809 519 + 2.147 1.993 -0.518
ENSG00000153561 E002 139.052292 0.0002236172 5.609619e-13 1.432438e-11 2 86740927 86741069 143 + 2.148 2.025 -0.412
ENSG00000153561 E003 0.000000       2 86748228 86748490 263 +      
ENSG00000153561 E004 149.008045 0.0012337598 7.108902e-11 1.285935e-09 2 86751896 86752030 135 + 2.175 2.055 -0.402
ENSG00000153561 E005 146.576314 0.0037000189 3.464356e-05 2.027907e-04 2 86753458 86753558 101 + 2.155 2.089 -0.221
ENSG00000153561 E006 185.574448 0.0026708737 4.832546e-03 1.546524e-02 2 86765027 86765193 167 + 2.235 2.243 0.029
ENSG00000153561 E007 0.000000       2 86765513 86765602 90 +      
ENSG00000153561 E008 199.326182 0.0007272993 7.539092e-07 6.430989e-06 2 86765859 86766024 166 + 2.276 2.246 -0.100
ENSG00000153561 E009 172.732259 0.0002426798 1.177471e-04 6.035402e-04 2 86770023 86770125 103 + 2.204 2.210 0.020
ENSG00000153561 E010 0.147249 0.0451825703 1.000000e+00   2 86771301 86771450 150 + 0.078 0.001 -7.314
ENSG00000153561 E011 191.658724 0.0004861045 3.755500e-02 8.650713e-02 2 86771558 86771712 155 + 2.232 2.290 0.193
ENSG00000153561 E012 2146.292751 0.0039309626 3.678331e-12 8.219495e-11 2 86773348 86778041 4694 + 3.227 3.424 0.655