ENSG00000153558

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000435207 ENSG00000153558 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXL2 protein_coding nonsense_mediated_decay 3.832649 2.249164 4.658306 0.2064681 0.2786618 1.04711 0.8687543 0.5740529 1.4622031 0.09686994 0.14299164 1.3338058 0.18967917 0.25740000 0.3125667 0.05516667 0.62648230 0.01959124 FALSE TRUE
ENST00000464990 ENSG00000153558 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXL2 protein_coding retained_intron 3.832649 2.249164 4.658306 0.2064681 0.2786618 1.04711 0.1999890 0.1365040 0.1890475 0.01380184 0.01878197 0.4421725 0.05860833 0.06123333 0.0404000 -0.02083333 0.58060200 0.01959124 TRUE TRUE
ENST00000484457 ENSG00000153558 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXL2 protein_coding protein_coding 3.832649 2.249164 4.658306 0.2064681 0.2786618 1.04711 1.8444799 1.3233854 1.8166358 0.14469925 0.07981340 0.4540952 0.51515417 0.58656667 0.3937000 -0.19286667 0.02425195 0.01959124 FALSE TRUE
MSTRG.22686.2 ENSG00000153558 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXL2 protein_coding   3.832649 2.249164 4.658306 0.2064681 0.2786618 1.04711 0.3041169 0.0380233 0.4769728 0.03802330 0.05205196 3.3420348 0.06863750 0.01456667 0.1038333 0.08926667 0.01959124 0.01959124 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000153558 E001 0.0000000       3 33277025 33277096 72 +      
ENSG00000153558 E002 0.0000000       3 33277427 33277427 1 +      
ENSG00000153558 E003 0.2966881 0.0290785164 0.3693618738   3 33277428 33277441 14 + 0.181 0.000 -10.227
ENSG00000153558 E004 0.2966881 0.0290785164 0.3693618738   3 33277442 33277444 3 + 0.181 0.000 -12.546
ENSG00000153558 E005 0.2966881 0.0290785164 0.3693618738   3 33277445 33277445 1 + 0.181 0.000 -12.546
ENSG00000153558 E006 0.7363589 0.0155917745 0.0563953901 0.120416314 3 33277446 33277450 5 + 0.360 0.000 -13.790
ENSG00000153558 E007 0.7363589 0.0155917745 0.0563953901 0.120416314 3 33277451 33277452 2 + 0.360 0.000 -13.790
ENSG00000153558 E008 2.3812396 0.3185543100 0.6030516364 0.723904187 3 33277453 33277465 13 + 0.554 0.488 -0.315
ENSG00000153558 E009 2.6757377 0.3501255820 0.4805800349 0.618839098 3 33277466 33277471 6 + 0.612 0.488 -0.577
ENSG00000153558 E010 2.8209103 0.3493241262 0.4264678812 0.569289952 3 33277472 33277475 4 + 0.638 0.488 -0.691
ENSG00000153558 E011 3.1938710 0.1396555418 0.5410657857 0.672105117 3 33277476 33277476 1 + 0.664 0.544 -0.534
ENSG00000153558 E012 6.6885685 0.0156997200 0.5489451009 0.678749764 3 33277477 33277515 39 + 0.915 0.829 -0.331
ENSG00000153558 E013 0.1515154 0.0429836038 0.7695498547   3 33278173 33278393 221 + 0.100 0.000 -11.533
ENSG00000153558 E014 0.0000000       3 33289650 33289668 19 +      
ENSG00000153558 E015 0.0000000       3 33289669 33289795 127 +      
ENSG00000153558 E016 15.1807129 0.0213803661 0.2241340650 0.355350368 3 33297664 33297725 62 + 1.151 1.270 0.421
ENSG00000153558 E017 0.3332198 0.0283119842 0.7304481333   3 33297726 33297803 78 + 0.100 0.149 0.666
ENSG00000153558 E018 3.2140163 0.0511546126 0.7399728696 0.829510646 3 33297869 33297962 94 + 0.585 0.635 0.223
ENSG00000153558 E019 0.0000000       3 33300522 33300571 50 +      
ENSG00000153558 E020 0.4375944 0.0256440353 0.1889317114 0.312410390 3 33303074 33303160 87 + 0.249 0.000 -13.096
ENSG00000153558 E021 0.0000000       3 33317477 33317613 137 +      
ENSG00000153558 E022 16.8295673 0.0011977056 0.8956578049 0.937349842 3 33358967 33359021 55 + 1.263 1.250 -0.044
ENSG00000153558 E023 23.7241539 0.0008204496 0.5902640664 0.713148069 3 33359283 33359357 75 + 1.412 1.372 -0.139
ENSG00000153558 E024 0.1451727 0.0428916585 0.7696669700   3 33359358 33359358 1 + 0.100 0.000 -11.533
ENSG00000153558 E025 0.0000000       3 33364336 33364624 289 +      
ENSG00000153558 E026 28.1611779 0.0006860199 0.7575764059 0.842352227 3 33364625 33364719 95 + 1.476 1.454 -0.076
ENSG00000153558 E027 0.1451727 0.0428916585 0.7696669700   3 33372341 33372675 335 + 0.100 0.000 -11.533
ENSG00000153558 E028 0.0000000       3 33372988 33373020 33 +      
ENSG00000153558 E029 0.1482932 0.0411597534 0.3094354507   3 33373021 33373091 71 + 0.000 0.149 11.393
ENSG00000153558 E030 23.9884892 0.0007960595 0.4051832072 0.548867332 3 33373092 33373160 69 + 1.425 1.364 -0.210
ENSG00000153558 E031 0.4804688 0.0222033396 0.8784362074 0.925918650 3 33373241 33373259 19 + 0.181 0.149 -0.334
ENSG00000153558 E032 18.0979646 0.0010794807 0.3436630916 0.487721490 3 33373260 33373275 16 + 1.309 1.230 -0.278
ENSG00000153558 E033 18.4710532 0.0070994137 0.2614130932 0.399064676 3 33373276 33373302 27 + 1.326 1.220 -0.370
ENSG00000153558 E034 23.9189493 0.0008725055 0.1220792338 0.222905350 3 33373303 33373355 53 + 1.437 1.324 -0.392
ENSG00000153558 E035 0.0000000       3 33373356 33373577 222 +      
ENSG00000153558 E036 29.3962650 0.0007329633 0.3883660727 0.532447266 3 33373578 33373704 127 + 1.505 1.448 -0.197
ENSG00000153558 E037 11.9049148 0.0017914324 0.4397768085 0.581517991 3 33373847 33373850 4 + 1.144 1.066 -0.278
ENSG00000153558 E038 15.7747590 0.0068073211 0.7829027667 0.860601129 3 33373851 33373870 20 + 1.211 1.230 0.070
ENSG00000153558 E039 19.3615732 0.0012520320 0.3721588953 0.516615841 3 33373871 33373921 51 + 1.275 1.341 0.230
ENSG00000153558 E040 0.0000000       3 33373922 33373924 3 +      
ENSG00000153558 E041 28.2164174 0.0007463844 0.7772143873 0.856609734 3 33375288 33375418 131 + 1.457 1.473 0.053
ENSG00000153558 E042 24.1016016 0.0008060730 0.0485826383 0.106650542 3 33377273 33377333 61 + 1.457 1.315 -0.492
ENSG00000153558 E043 23.3855505 0.0008751704 0.3243066761 0.467663579 3 33378103 33378147 45 + 1.420 1.348 -0.250
ENSG00000153558 E044 24.2934034 0.0008677530 0.7865794110 0.863320199 3 33378685 33378741 57 + 1.398 1.415 0.056
ENSG00000153558 E045 1.3588919 0.1491567059 0.4140333550 0.557451847 3 33378742 33378834 93 + 0.448 0.259 -1.148
ENSG00000153558 E046 8.2013386 0.0021044267 0.3441340874 0.488191839 3 33382552 33383988 1437 + 0.916 1.018 0.381
ENSG00000153558 E047 42.2946948 0.0057458350 0.5870458495 0.710441041 3 33383989 33384201 213 + 1.624 1.657 0.109
ENSG00000153558 E048 41.6583529 0.0051297953 0.2248030477 0.356196372 3 33385501 33385728 228 + 1.600 1.672 0.246
ENSG00000153558 E049 95.8071447 0.0003268105 0.0002586831 0.001211329 3 33385729 33388092 2364 + 1.931 2.049 0.398
ENSG00000153558 E050 2.8795671 0.0174574891 0.1173335046 0.216142047 3 33403234 33404018 785 + 0.688 0.421 -1.244