ENSG00000152778

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371795 ENSG00000152778 HEK293_OSMI2_2hA HEK293_TMG_2hB IFIT5 protein_coding protein_coding 2.774206 1.056663 5.145671 0.1917875 0.3185732 2.273055 1.65898613 0.9331062 2.601253 0.1666644 0.07444844 1.469250 0.7114667 0.9014 0.5078333 -0.3935667 7.869063e-02 5.045076e-16 FALSE TRUE
ENST00000681163 ENSG00000152778 HEK293_OSMI2_2hA HEK293_TMG_2hB IFIT5 protein_coding protein_coding 2.774206 1.056663 5.145671 0.1917875 0.3185732 2.273055 1.05261948 0.0000000 2.544418 0.0000000 0.25157093 7.996851 0.2519542 0.0000 0.4921667 0.4921667 5.045076e-16 5.045076e-16 FALSE TRUE
ENST00000681422 ENSG00000152778 HEK293_OSMI2_2hA HEK293_TMG_2hB IFIT5 protein_coding protein_coding 2.774206 1.056663 5.145671 0.1917875 0.3185732 2.273055 0.01544465 0.1235572 0.000000 0.1235572 0.00000000 -3.739386 0.0123250 0.0986 0.0000000 -0.0986000 4.161310e-01 5.045076e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152778 E001 1.2586448 0.0255349445 2.968294e-01 4.381012e-01 10 89414568 89414731 164 + 0.352 0.203 -1.073
ENSG00000152778 E002 1.2692540 0.1537061353 3.663438e-01 5.106823e-01 10 89414732 89414747 16 + 0.353 0.204 -1.065
ENSG00000152778 E003 5.1392065 0.0032395454 7.699082e-02 1.546708e-01 10 89414748 89414761 14 + 0.778 0.598 -0.753
ENSG00000152778 E004 11.9048323 0.0512596819 1.648719e-01 2.815339e-01 10 89414762 89414803 42 + 1.076 0.979 -0.353
ENSG00000152778 E005 0.0000000       10 89414884 89414913 30 +      
ENSG00000152778 E006 0.0000000       10 89414998 89415139 142 +      
ENSG00000152778 E007 0.2998086 0.0290092632 4.945787e-01   10 89417188 89417204 17 + 0.071 0.202 1.729
ENSG00000152778 E008 45.1880523 0.0007674344 2.249208e-06 1.730028e-05 10 89417205 89417551 347 + 1.647 1.487 -0.549
ENSG00000152778 E009 28.4530017 0.0008446779 1.488674e-02 4.023464e-02 10 89417552 89417673 122 + 1.433 1.372 -0.210
ENSG00000152778 E010 27.7228215 0.0014710658 9.494136e-01 9.723353e-01 10 89417674 89417817 144 + 1.371 1.487 0.399
ENSG00000152778 E011 35.3386043 0.0084547609 5.532679e-01 6.824436e-01 10 89417818 89418063 246 + 1.484 1.552 0.235
ENSG00000152778 E012 120.2828590 0.0547630327 2.813555e-02 6.828320e-02 10 89418064 89420997 2934 + 1.937 2.218 0.941
ENSG00000152778 E013 0.1515154 0.0438486285 1.000000e+00   10 89421257 89421286 30 + 0.071 0.000 -8.074
ENSG00000152778 E014 0.4460135 0.0380389186 3.615844e-01 5.059799e-01 10 89421485 89421605 121 + 0.186 0.000 -9.659