ENSG00000152749

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376958 ENSG00000152749 HEK293_OSMI2_2hA HEK293_TMG_2hB GPR180 protein_coding protein_coding 16.48627 2.027333 28.59017 0.1822662 1.08011 3.81127 4.8920746 1.5172849 8.526679 0.2525688 0.06625044 2.482706 0.4525208 0.7387667 0.2989667 -0.4398000 2.456783e-08 2.456783e-08 FALSE TRUE
MSTRG.8950.1 ENSG00000152749 HEK293_OSMI2_2hA HEK293_TMG_2hB GPR180 protein_coding   16.48627 2.027333 28.59017 0.1822662 1.08011 3.81127 10.5507410 0.2806639 18.057521 0.1437794 1.11423308 5.957903 0.4587417 0.1399333 0.6305667 0.4906333 1.706443e-02 2.456783e-08 TRUE TRUE
MSTRG.8950.2 ENSG00000152749 HEK293_OSMI2_2hA HEK293_TMG_2hB GPR180 protein_coding   16.48627 2.027333 28.59017 0.1822662 1.08011 3.81127 0.6141804 0.2293839 1.159649 0.1806382 0.17144888 2.288678 0.0573625 0.1213000 0.0406000 -0.0807000 9.186543e-01 2.456783e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152749 E001 1.768374 0.0111441422 6.766141e-01 7.815483e-01 13 94601812 94601856 45 + 0.339 0.297 -0.269
ENSG00000152749 E002 59.052604 0.0004137000 8.145025e-03 2.415054e-02 13 94601857 94602072 216 + 1.600 1.512 -0.301
ENSG00000152749 E003 43.457326 0.0004728018 2.695277e-02 6.589395e-02 13 94605391 94605415 25 + 1.472 1.391 -0.280
ENSG00000152749 E004 94.699844 0.0002938761 7.658169e-06 5.224405e-05 13 94605416 94605549 134 + 1.809 1.646 -0.552
ENSG00000152749 E005 146.371803 0.0002463147 4.619755e-12 1.015312e-10 13 94612190 94612390 201 + 2.001 1.756 -0.823
ENSG00000152749 E006 117.251635 0.0018083994 3.158161e-05 1.869010e-04 13 94619150 94619330 181 + 1.897 1.764 -0.448
ENSG00000152749 E007 70.646234 0.0037842402 1.473427e-02 3.989086e-02 13 94619468 94619517 50 + 1.674 1.596 -0.267
ENSG00000152749 E008 121.481745 0.0027980492 6.200263e-06 4.322433e-05 13 94621078 94621235 158 + 1.914 1.749 -0.559
ENSG00000152749 E009 160.822406 0.0002035574 3.401751e-07 3.112841e-06 13 94623109 94623300 192 + 2.033 1.912 -0.406
ENSG00000152749 E010 100.442986 0.0002980143 1.348924e-01 2.409414e-01 13 94625966 94626043 78 + 1.816 1.844 0.096
ENSG00000152749 E011 173.850398 0.0002019735 1.089810e-02 3.091893e-02 13 94627013 94627337 325 + 2.052 2.057 0.018
ENSG00000152749 E012 880.492684 0.0032836342 1.370105e-24 1.438816e-22 13 94627338 94634661 7324 + 2.710 2.996 0.953
ENSG00000152749 E013 0.147249 0.0449457080 7.700036e-01   13 94641384 94641440 57 + 0.044 0.001 -5.290