ENSG00000152558

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000398136 ENSG00000152558 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM123 protein_coding protein_coding 71.40644 10.36657 131.1806 1.169038 5.070533 3.660262 39.90551 10.36657 70.50417 1.169038 2.440098 2.764583 0.7283292 1 0.5378000 -0.4622000 1.823300e-75 1.8233e-75 FALSE TRUE
ENST00000528969 ENSG00000152558 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM123 protein_coding protein_coding 71.40644 10.36657 131.1806 1.169038 5.070533 3.660262 31.24018 0.00000 59.82240 0.000000 3.293308 12.546711 0.2679042 0 0.4556333 0.4556333 8.733402e-73 1.8233e-75 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152558 E001 2456.9138042 0.0048729825 1.199866e-14 3.899656e-13 11 102396332 102398823 2492 - 3.152 3.410 0.859
ENSG00000152558 E002 153.1612065 0.0002094247 1.501449e-01 2.618676e-01 11 102398824 102398855 32 - 1.966 2.145 0.601
ENSG00000152558 E003 177.7831846 0.0001961991 8.773658e-01 9.252171e-01 11 102398856 102398891 36 - 2.039 2.154 0.387
ENSG00000152558 E004 466.6077213 0.0001470694 6.899568e-09 8.764264e-08 11 102401539 102401692 154 - 2.473 2.446 -0.089
ENSG00000152558 E005 0.0000000       11 102401744 102401915 172 -      
ENSG00000152558 E006 252.0606771 0.0002338244 5.970061e-07 5.202439e-06 11 102401916 102401924 9 - 2.210 2.151 -0.196
ENSG00000152558 E007 512.5600331 0.0001804141 3.283535e-12 7.404545e-11 11 102401925 102402033 109 - 2.515 2.463 -0.175
ENSG00000152558 E008 393.8546718 0.0001534778 9.803905e-13 2.411165e-11 11 102402034 102402098 65 - 2.404 2.319 -0.286
ENSG00000152558 E009 407.0632962 0.0002633281 5.020944e-13 1.292571e-11 11 102402099 102402206 108 - 2.419 2.329 -0.298
ENSG00000152558 E010 1.6347285 0.0792513505 2.263665e-01 3.580507e-01 11 102419074 102419154 81 - 0.333 0.000 -10.213
ENSG00000152558 E011 0.3729606 0.0298059987 1.490710e-01 2.604134e-01 11 102448236 102448375 140 - 0.043 0.304 3.276
ENSG00000152558 E012 234.0788518 0.0059560628 3.649407e-05 2.123435e-04 11 102448812 102448868 57 - 2.181 2.071 -0.368
ENSG00000152558 E013 0.0000000       11 102449115 102449452 338 -      
ENSG00000152558 E014 0.2903454 0.3322117046 1.000000e+00   11 102451202 102451231 30 - 0.082 0.000 -7.764
ENSG00000152558 E015 0.1515154 0.0427066863 7.535431e-01   11 102452194 102452523 330 - 0.043 0.000 -6.750
ENSG00000152558 E016 218.4268876 0.0206577231 8.762810e-03 2.569111e-02 11 102452524 102452839 316 - 2.151 2.047 -0.346
ENSG00000152558 E017 0.0000000       11 102470174 102470384 211 -