ENSG00000152527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000282406 ENSG00000152527 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH2 protein_coding protein_coding 1.01986 0.5931915 1.027683 0.03896753 0.09662962 0.782678 0.42059317 0.17853132 0.67583674 0.053504644 0.05228231 1.863061 0.43402917 0.29213333 0.66036667 0.36823333 4.526836e-03 6.222498e-11 FALSE TRUE
ENST00000405000 ENSG00000152527 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH2 protein_coding retained_intron 1.01986 0.5931915 1.027683 0.03896753 0.09662962 0.782678 0.12351520 0.04071624 0.11684919 0.008853303 0.01428527 1.322595 0.11565000 0.07046667 0.11803333 0.04756667 7.164572e-01 6.222498e-11 TRUE TRUE
ENST00000405223 ENSG00000152527 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH2 protein_coding retained_intron 1.01986 0.5931915 1.027683 0.03896753 0.09662962 0.782678 0.05060176 0.00000000 0.04332386 0.000000000 0.04332386 2.414781 0.04428333 0.00000000 0.05173333 0.05173333 9.023631e-01 6.222498e-11 FALSE TRUE
ENST00000490038 ENSG00000152527 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH2 protein_coding nonsense_mediated_decay 1.01986 0.5931915 1.027683 0.03896753 0.09662962 0.782678 0.15956460 0.37394398 0.00000000 0.015514557 0.00000000 -5.262824 0.20578333 0.63740000 0.00000000 -0.63740000 6.222498e-11 6.222498e-11 FALSE TRUE
ENST00000493408 ENSG00000152527 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH2 protein_coding retained_intron 1.01986 0.5931915 1.027683 0.03896753 0.09662962 0.782678 0.19847370 0.00000000 0.16024332 0.000000000 0.10237516 4.089526 0.14519583 0.00000000 0.14110000 0.14110000 3.077935e-01 6.222498e-11 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152527 E001 0.2966881 0.027546994 5.412936e-01   2 43637260 43637299 40 + 0.156 0.000 -9.575
ENSG00000152527 E002 0.5870335 0.174223388 2.344590e-01 3.677102e-01 2 43637300 43637307 8 + 0.271 0.000 -12.519
ENSG00000152527 E003 0.7322062 0.322868202 2.225062e-01 3.533816e-01 2 43637308 43637338 31 + 0.318 0.000 -12.644
ENSG00000152527 E004 1.0309706 0.022076267 5.116790e-02 1.112430e-01 2 43637339 43637379 41 + 0.401 0.000 -13.259
ENSG00000152527 E005 3.6902239 0.004312312 4.212348e-02 9.494845e-02 2 43644671 43644796 126 + 0.744 0.392 -1.633
ENSG00000152527 E006 0.3686942 0.028356090 5.375350e-01 6.690828e-01 2 43658666 43658867 202 + 0.085 0.173 1.177
ENSG00000152527 E007 3.3914594 0.004628769 7.098346e-02 1.449736e-01 2 43678863 43678925 63 + 0.709 0.392 -1.488
ENSG00000152527 E008 0.0000000       2 43679517 43679638 122 +      
ENSG00000152527 E009 0.0000000       2 43692230 43692513 284 +      
ENSG00000152527 E010 4.6467934 0.021191696 4.432709e-01 5.848302e-01 2 43692514 43692663 150 + 0.777 0.646 -0.542
ENSG00000152527 E011 2.7742490 0.005470714 4.563981e-01 5.968293e-01 2 43694431 43694514 84 + 0.606 0.471 -0.634
ENSG00000152527 E012 3.2053869 0.017160067 2.690134e-01 4.074723e-01 2 43695143 43695224 82 + 0.670 0.470 -0.914
ENSG00000152527 E013 1.6188208 0.071435696 1.239296e-01 2.255709e-01 2 43697171 43697173 3 + 0.500 0.172 -2.152
ENSG00000152527 E014 5.3348775 0.057691878 6.628459e-01 7.710701e-01 2 43697174 43697356 183 + 0.821 0.729 -0.367
ENSG00000152527 E015 15.2891953 0.002005464 1.432892e-02 3.897618e-02 2 43699647 43700608 962 + 1.258 1.013 -0.881
ENSG00000152527 E016 3.4159119 0.039320006 2.497667e-01 3.856424e-01 2 43703981 43704056 76 + 0.691 0.471 -0.996
ENSG00000152527 E017 3.1891536 0.009565101 6.195527e-01 7.369398e-01 2 43706322 43706416 95 + 0.628 0.537 -0.410
ENSG00000152527 E018 2.8882122 0.079031600 8.405217e-01 9.004683e-01 2 43707401 43707545 145 + 0.582 0.540 -0.195
ENSG00000152527 E019 1.9197859 0.007489922 8.100196e-01 8.794150e-01 2 43709990 43710126 137 + 0.437 0.471 0.174
ENSG00000152527 E020 2.1296784 0.176830847 9.416894e-01 9.673411e-01 2 43710220 43710330 111 + 0.469 0.477 0.043
ENSG00000152527 E021 2.3907784 0.006604038 7.712295e-01 8.522994e-01 2 43710489 43710575 87 + 0.529 0.471 -0.284
ENSG00000152527 E022 4.0319754 0.005201886 4.269850e-01 5.698277e-01 2 43710576 43712224 1649 + 0.727 0.595 -0.563
ENSG00000152527 E023 4.2169020 0.004901851 3.613035e-01 5.056989e-01 2 43712225 43712383 159 + 0.744 0.595 -0.633
ENSG00000152527 E024 3.8862390 0.175075320 1.678052e-01 2.853394e-01 2 43720669 43720749 81 + 0.763 0.395 -1.692
ENSG00000152527 E025 5.9071904 0.036546434 1.613293e-01 2.768176e-01 2 43726272 43726451 180 + 0.888 0.646 -0.973
ENSG00000152527 E026 4.0503973 0.004436246 4.259817e-01 5.688534e-01 2 43729637 43729745 109 + 0.727 0.595 -0.562
ENSG00000152527 E027 1.5707601 0.147433742 5.723937e-01 6.985133e-01 2 43731490 43731540 51 + 0.436 0.299 -0.804
ENSG00000152527 E028 1.0987104 0.077990163 3.644786e-01 5.088384e-01 2 43731541 43731602 62 + 0.361 0.174 -1.401
ENSG00000152527 E029 2.6960019 0.005864111 9.215911e-03 2.682451e-02 2 43738341 43738520 180 + 0.671 0.173 -2.913
ENSG00000152527 E030 0.1515154 0.042929379 1.000000e+00   2 43740816 43740945 130 + 0.085 0.000 -11.032
ENSG00000152527 E031 2.4755863 0.006109568 9.772227e-02 1.870208e-01 2 43740946 43740984 39 + 0.606 0.296 -1.633
ENSG00000152527 E032 3.1742781 0.004850599 6.145771e-01 7.329820e-01 2 43740985 43741043 59 + 0.628 0.537 -0.410
ENSG00000152527 E033 0.7655156 0.308853695 6.229524e-01 7.396561e-01 2 43741044 43741126 83 + 0.217 0.294 0.578
ENSG00000152527 E034 0.2903454 0.396998930 6.580505e-01   2 43741127 43741365 239 + 0.156 0.000 -11.503
ENSG00000152527 E035 4.9381684 0.019808568 9.132962e-01 9.490496e-01 2 43742741 43742918 178 + 0.761 0.733 -0.111
ENSG00000152527 E036 5.6215362 0.003811174 2.916194e-01 4.325014e-01 2 43743834 43743989 156 + 0.849 0.691 -0.633
ENSG00000152527 E037 0.4439371 0.021649704 3.095742e-01 4.518920e-01 2 43743990 43744134 145 + 0.217 0.000 -12.384
ENSG00000152527 E038 2.8525978 0.005888622 8.317431e-01 8.945693e-01 2 43745866 43745898 33 + 0.582 0.537 -0.204
ENSG00000152527 E039 3.9417668 0.004588610 2.324565e-01 3.653650e-01 2 43745899 43745963 65 + 0.606 0.770 0.690
ENSG00000152527 E040 4.7397501 0.006533587 3.756370e-01 5.200475e-01 2 43753619 43753760 142 + 0.690 0.805 0.466
ENSG00000152527 E041 5.8908999 0.003181054 6.760502e-01 7.811493e-01 2 43757119 43757264 146 + 0.792 0.837 0.176
ENSG00000152527 E042 7.4567784 0.016948705 3.656817e-01 5.099875e-01 2 43758900 43759029 130 + 0.863 0.970 0.408
ENSG00000152527 E043 6.8623344 0.002468636 9.844713e-01 9.943705e-01 2 43762304 43762390 87 + 0.875 0.867 -0.032
ENSG00000152527 E044 5.6421707 0.003479801 3.725139e-01 5.169603e-01 2 43764228 43764365 138 + 0.761 0.867 0.416
ENSG00000152527 E045 46.4017560 0.001703013 3.264970e-14 9.957159e-13 2 43765413 43767987 2575 + 1.502 1.842 1.155