ENSG00000152484

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000282344 ENSG00000152484 HEK293_OSMI2_2hA HEK293_TMG_2hB USP12 protein_coding protein_coding 6.964316 3.172811 10.4019 0.2878294 0.2746846 1.70986 6.3882580 2.4055064 9.9898439 0.1762300 0.4126958 2.0495799 0.8712042 0.7606333 0.95963333 0.1990000 0.0001555431 0.0001555431 FALSE TRUE
ENST00000620323 ENSG00000152484 HEK293_OSMI2_2hA HEK293_TMG_2hB USP12 protein_coding protein_coding 6.964316 3.172811 10.4019 0.2878294 0.2746846 1.70986 0.5479119 0.7534249 0.4120589 0.1038277 0.1386983 -0.8550418 0.1234125 0.2356333 0.04036667 -0.1952667 0.0001775691 0.0001555431   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152484 E001 482.6239089 0.0296000255 2.136867e-04 1.022745e-03 13 27066156 27069384 3229 - 2.487 2.800 1.041
ENSG00000152484 E002 50.0079837 0.0161520599 4.070280e-02 9.232802e-02 13 27071071 27071149 79 - 1.580 1.574 -0.019
ENSG00000152484 E003 75.7384995 0.0305703584 5.069353e-02 1.103970e-01 13 27075191 27075388 198 - 1.758 1.749 -0.032
ENSG00000152484 E004 0.1482932 0.0410320372 1.071565e-01   13 27089438 27089882 445 - 0.000 0.255 11.775
ENSG00000152484 E005 38.2755034 0.0490275600 1.236034e-01 2.250918e-01 13 27089883 27089966 84 - 1.470 1.450 -0.071
ENSG00000152484 E006 33.9212460 0.0006493829 1.797550e-02 4.708280e-02 13 27090082 27090158 77 - 1.419 1.412 -0.023
ENSG00000152484 E007 65.2177634 0.0073242693 1.065778e-03 4.186713e-03 13 27095601 27095830 230 - 1.704 1.653 -0.172
ENSG00000152484 E008 67.5446480 0.0003659792 5.874284e-09 7.575151e-08 13 27105731 27105944 214 - 1.734 1.569 -0.560
ENSG00000152484 E009 31.5984898 0.0041158678 2.349567e-06 1.799476e-05 13 27116516 27116596 81 - 1.423 1.141 -0.992
ENSG00000152484 E010 4.1964186 0.0038606242 2.936868e-07 2.723352e-06 13 27170091 27170329 239 - 0.313 1.165 3.687
ENSG00000152484 E011 24.4825154 0.0086792262 1.949742e-01 3.199537e-01 13 27171592 27171811 220 - 1.275 1.323 0.166