ENSG00000152422

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000338635 ENSG00000152422 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC4 protein_coding protein_coding 5.307425 1.789286 8.791957 0.2755589 0.5493916 2.2904 3.9043390 1.2915151 5.394614 0.1215094 0.3702559 2.054000 0.7342917 0.7352667 0.6225333 -0.1127333 0.633124648 0.003528935 FALSE TRUE
ENST00000511817 ENSG00000152422 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC4 protein_coding protein_coding 5.307425 1.789286 8.791957 0.2755589 0.5493916 2.2904 0.6419935 0.0330411 2.247128 0.0330411 0.5595839 5.712630 0.0851000 0.0233000 0.2497333 0.2264333 0.003528935 0.003528935 FALSE TRUE
ENST00000542685 ENSG00000152422 HEK293_OSMI2_2hA HEK293_TMG_2hB XRCC4 protein_coding processed_transcript 5.307425 1.789286 8.791957 0.2755589 0.5493916 2.2904 0.7266458 0.4557566 1.034786 0.1805401 0.2757111 1.165559 0.1733667 0.2376000 0.1152667 -0.1223333 0.247277312 0.003528935 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152422 E001 0.1451727 0.0435340581 1.000000e+00   5 83077498 83077526 29 + 0.062 0.000 -7.705
ENSG00000152422 E002 0.1451727 0.0435340581 1.000000e+00   5 83077527 83077546 20 + 0.062 0.000 -7.647
ENSG00000152422 E003 0.2966881 0.0269346378 1.000000e+00   5 83077547 83077556 10 + 0.117 0.000 -8.646
ENSG00000152422 E004 3.8052196 0.0040465652 7.402048e-01 0.8296822295 5 83077557 83077592 36 + 0.628 0.573 -0.248
ENSG00000152422 E005 12.2260325 0.0017796287 6.776818e-02 0.1396610055 5 83077593 83077615 23 + 0.992 1.187 0.704
ENSG00000152422 E006 3.0795716 0.0143155228 3.073041e-01 0.4495169664 5 83077616 83077662 47 + 0.578 0.375 -1.024
ENSG00000152422 E007 0.0000000       5 83104908 83104909 2 +      
ENSG00000152422 E008 37.8415460 0.0007650374 1.899918e-01 0.3137562621 5 83104910 83105058 149 + 1.521 1.432 -0.307
ENSG00000152422 E009 49.5295095 0.0005363043 1.250116e-03 0.0048106009 5 83111028 83111203 176 + 1.656 1.453 -0.693
ENSG00000152422 E010 45.2592280 0.0005301650 4.349254e-01 0.5771717927 5 83195770 83195936 167 + 1.592 1.547 -0.154
ENSG00000152422 E011 43.4781438 0.0005444355 6.187013e-01 0.7362552919 5 83203552 83203707 156 + 1.562 1.594 0.112
ENSG00000152422 E012 38.6130969 0.0005616767 5.599474e-01 0.6880114561 5 83204815 83204921 107 + 1.507 1.547 0.138
ENSG00000152422 E013 45.7638597 0.0004999320 9.168278e-01 0.9514563969 5 83258530 83258677 148 + 1.590 1.587 -0.011
ENSG00000152422 E014 0.7353267 0.0484265546 1.000000e+00 1.0000000000 5 83258678 83259339 662 + 0.209 0.226 0.141
ENSG00000152422 E015 4.4463712 0.0352619615 2.265006e-05 0.0001386605 5 83310789 83311069 281 + 0.432 1.096 2.752
ENSG00000152422 E016 15.7388239 0.0011641774 7.393009e-01 0.8289879376 5 83353125 83353130 6 + 1.156 1.124 -0.112
ENSG00000152422 E017 60.5362478 0.0004099520 7.576020e-02 0.1527191117 5 83353131 83353787 657 + 1.687 1.777 0.304