ENSG00000152256

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000282077 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding protein_coding 7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.9526366 0.34502268 1.7740181 0.06362948 0.09961718 2.3291472 0.11530833 0.09780000 0.12946667 0.03166667 4.946100e-01 2.419772e-06 FALSE TRUE
ENST00000392571 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding protein_coding 7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.7662075 0.03620505 2.2696985 0.03620505 0.28075378 5.6246487 0.05712500 0.01223333 0.16633333 0.15410000 2.419772e-06 2.419772e-06 FALSE TRUE
MSTRG.19501.11 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.1874804 0.44559221 0.0000000 0.28466372 0.00000000 -5.5096712 0.04098333 0.11170000 0.00000000 -0.11170000 3.364213e-02 2.419772e-06 TRUE TRUE
MSTRG.19501.12 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 2.5680714 1.32864055 3.1348666 0.45883537 0.47248536 1.2322302 0.36233750 0.36453333 0.23063333 -0.13390000 5.523345e-01 2.419772e-06 TRUE TRUE
MSTRG.19501.13 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.9041472 0.22494842 1.1998111 0.22494842 0.34916175 2.3643659 0.08622500 0.07600000 0.08700000 0.01100000 4.867880e-01 2.419772e-06 TRUE TRUE
MSTRG.19501.14 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.3578390 0.38963848 0.4829585 0.21213992 0.16368739 0.3027706 0.05846250 0.10356667 0.03583333 -0.06773333 6.822522e-01 2.419772e-06 TRUE TRUE
MSTRG.19501.3 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.2116698 0.19926053 0.0000000 0.19926053 0.00000000 -4.3872283 0.05621250 0.06510000 0.00000000 -0.06510000 5.960081e-01 2.419772e-06 TRUE TRUE
MSTRG.19501.7 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.6390417 0.00000000 1.6119594 0.00000000 0.62271886 7.3415939 0.08014583 0.00000000 0.11630000 0.11630000 2.836904e-03 2.419772e-06 TRUE TRUE
MSTRG.19501.8 ENSG00000152256 HEK293_OSMI2_2hA HEK293_TMG_2hB PDK1 protein_coding   7.947394 3.505086 13.6921 0.4951399 0.3155932 1.962765 0.7403316 0.33240989 2.4421813 0.33240989 0.27850354 2.8402694 0.06810417 0.11230000 0.17790000 0.06560000 3.148501e-01 2.419772e-06 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152256 E001 0.0000000       2 172555373 172555626 254 +      
ENSG00000152256 E002 0.1515154 0.0440256800 1.000000e+00   2 172555627 172555719 93 + 0.069 0.000 -8.734
ENSG00000152256 E003 1.1962011 0.4969484369 6.866987e-01 7.891647e-01 2 172556007 172556050 44 + 0.270 0.363 0.597
ENSG00000152256 E004 1.1962011 0.4969484369 6.866987e-01 7.891647e-01 2 172556051 172556053 3 + 0.270 0.363 0.597
ENSG00000152256 E005 1.8281854 0.3634282033 5.850987e-01 7.088558e-01 2 172556054 172556077 24 + 0.377 0.468 0.485
ENSG00000152256 E006 2.5540488 0.0199162128 7.929931e-01 8.676352e-01 2 172556078 172556089 12 + 0.509 0.456 -0.261
ENSG00000152256 E007 2.8443942 0.0386826066 6.542940e-01 7.645050e-01 2 172556090 172556101 12 + 0.553 0.457 -0.463
ENSG00000152256 E008 12.6498574 0.0599568063 2.534066e-02 6.257283e-02 2 172556102 172556177 76 + 1.147 0.731 -1.571
ENSG00000152256 E009 17.5403373 0.0010488083 1.536531e-04 7.633179e-04 2 172556178 172556253 76 + 1.283 0.856 -1.556
ENSG00000152256 E010 16.5558114 0.0010838542 3.284707e-02 7.750476e-02 2 172556254 172556312 59 + 1.233 1.012 -0.798
ENSG00000152256 E011 21.7082696 0.0009073206 1.764106e-02 4.636321e-02 2 172556313 172556346 34 + 1.344 1.126 -0.770
ENSG00000152256 E012 0.0000000       2 172556565 172556627 63 +      
ENSG00000152256 E013 50.0491992 0.0004887215 2.029261e-02 5.209531e-02 2 172558708 172558849 142 + 1.681 1.544 -0.467
ENSG00000152256 E014 39.1770279 0.0060744793 1.610455e-01 2.764826e-01 2 172562220 172562291 72 + 1.572 1.467 -0.360
ENSG00000152256 E015 8.6828211 0.0020847394 6.313779e-01 7.463848e-01 2 172562741 172562800 60 + 0.923 0.984 0.230
ENSG00000152256 E016 0.1451727 0.0441655651 1.000000e+00   2 172564052 172564130 79 + 0.069 0.000 -10.799
ENSG00000152256 E017 72.4453116 0.0040901958 7.257744e-01 8.189684e-01 2 172564503 172564687 185 + 1.815 1.795 -0.064
ENSG00000152256 E018 56.0461368 0.0004490558 7.397570e-01 8.293815e-01 2 172564978 172565073 96 + 1.693 1.713 0.068
ENSG00000152256 E019 52.4252831 0.0004159580 8.272830e-01 8.914121e-01 2 172566856 172566933 78 + 1.673 1.664 -0.032
ENSG00000152256 E020 51.0381717 0.0004256512 4.450540e-01 5.866107e-01 2 172568741 172568817 77 + 1.647 1.692 0.152
ENSG00000152256 E021 0.5181333 0.0210480416 6.225982e-01 7.393739e-01 2 172570585 172570725 141 + 0.128 0.209 0.852
ENSG00000152256 E022 64.0815992 0.0004029027 2.858366e-02 6.917761e-02 2 172570726 172570824 99 + 1.726 1.835 0.365
ENSG00000152256 E023 72.3066758 0.0003878862 4.437931e-03 1.436595e-02 2 172586278 172586388 111 + 1.773 1.903 0.440
ENSG00000152256 E024 70.4595445 0.0004242724 1.065694e-02 3.034974e-02 2 172592935 172593048 114 + 1.767 1.886 0.403
ENSG00000152256 E025 74.7400425 0.0004591867 2.229861e-02 5.629264e-02 2 172595829 172596023 195 + 1.795 1.900 0.354
ENSG00000152256 E026 174.9728567 0.0002145592 5.930453e-20 3.737547e-18 2 172596024 172597612 1589 + 2.244 1.939 -1.023
ENSG00000152256 E027 183.0488877 0.0009392909 3.951522e-04 1.755844e-03 2 172597613 172608669 11057 + 2.178 2.285 0.356
ENSG00000152256 E028 6.6972689 0.0436785172 2.404472e-07 2.269656e-06 2 172614407 172614505 99 + 0.486 1.249 3.022
ENSG00000152256 E029 2.2089854 0.0065508016 2.403165e-01 3.745064e-01 2 172621424 172621502 79 + 0.405 0.612 1.002
ENSG00000152256 E030 1.5091476 0.0130259207 5.574152e-01 6.858254e-01 2 172626517 172626612 96 + 0.342 0.455 0.626
ENSG00000152256 E031 8.6837783 0.0020111842 1.198619e-08 1.456542e-07 2 172627829 172627998 170 + 0.679 1.304 2.344
ENSG00000152256 E032 0.1482932 0.0411597534 1.212282e-01   2 172670795 172670850 56 + 0.000 0.209 12.339