ENSG00000152147

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409566 ENSG00000152147 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN6 protein_coding protein_coding 42.12482 41.5379 51.67913 5.631364 1.748277 0.3150854 8.751638 9.887762 7.527190 1.437143 0.4343982 -0.39307565 0.2150625 0.2384333 0.1465000 -0.09193333 0.01074492 0.01074492 FALSE TRUE
MSTRG.18251.2 ENSG00000152147 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN6 protein_coding   42.12482 41.5379 51.67913 5.631364 1.748277 0.3150854 25.128805 22.596803 34.565923 3.025396 2.5104062 0.61301078 0.5879125 0.5447333 0.6671667 0.12243333 0.04559018 0.01074492   FALSE
MSTRG.18251.3 ENSG00000152147 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN6 protein_coding   42.12482 41.5379 51.67913 5.631364 1.748277 0.3150854 6.554232 7.898476 7.632779 1.336904 0.5656996 -0.04930239 0.1551458 0.1885667 0.1484667 -0.04010000 0.26915899 0.01074492 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000152147 E001 0.1451727 0.0433045854 4.434159e-01   2 38751534 38751818 285 + 0.129 0.000 -8.881
ENSG00000152147 E002 0.0000000       2 38768105 38768199 95 +      
ENSG00000152147 E003 0.0000000       2 38778116 38778177 62 +      
ENSG00000152147 E004 2.8879945 0.0508875834 6.415572e-01 0.7545612233 2 38778178 38778204 27 + 0.533 0.637 0.468
ENSG00000152147 E005 10.4070003 0.0148288205 4.228440e-01 0.5659838192 2 38778205 38778209 5 + 1.105 1.031 -0.268
ENSG00000152147 E006 242.0320932 0.0002417570 1.922126e-03 0.0069841757 2 38778210 38778281 72 + 2.419 2.362 -0.188
ENSG00000152147 E007 467.9656520 0.0001335877 9.256501e-05 0.0004870615 2 38778972 38779118 147 + 2.699 2.654 -0.149
ENSG00000152147 E008 23.5014339 0.0214743613 1.238472e-02 0.0344589903 2 38779258 38779325 68 + 1.227 1.474 0.860
ENSG00000152147 E009 30.4574964 0.0195459279 1.968908e-03 0.0071287298 2 38779326 38779441 116 + 1.308 1.592 0.977
ENSG00000152147 E010 801.0276237 0.0012586403 1.319552e-01 0.2368338147 2 38781517 38782514 998 + 2.888 2.915 0.090
ENSG00000152147 E011 20.1316109 0.0023842039 8.911753e-04 0.0035833703 2 38782515 38783147 633 + 1.446 1.197 -0.869
ENSG00000152147 E012 177.9674711 0.0003962474 1.033533e-04 0.0005375864 2 38783148 38785002 1855 + 2.185 2.300 0.383
ENSG00000152147 E013 0.0000000       2 38786623 38786675 53 +