ENSG00000151893

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369151 ENSG00000151893 HEK293_OSMI2_2hA HEK293_TMG_2hB CACUL1 protein_coding protein_coding 10.50914 4.793009 14.91847 0.667381 0.5168437 1.636056 5.6905234 4.292364186 6.917804 0.624528865 0.23080257 0.6872685 0.6315750 0.89486667 0.4659000 -0.42896667 4.313628e-12 1.032563e-31 FALSE TRUE
ENST00000493518 ENSG00000151893 HEK293_OSMI2_2hA HEK293_TMG_2hB CACUL1 protein_coding nonsense_mediated_decay 10.50914 4.793009 14.91847 0.667381 0.5168437 1.636056 3.1266561 0.007322687 5.253619 0.007322687 0.26381769 8.2472485 0.1969833 0.00120000 0.3521333 0.35093333 1.032563e-31 1.032563e-31 TRUE TRUE
ENST00000544392 ENSG00000151893 HEK293_OSMI2_2hA HEK293_TMG_2hB CACUL1 protein_coding processed_transcript 10.50914 4.793009 14.91847 0.667381 0.5168437 1.636056 0.9019015 0.288272878 1.511201 0.110293361 0.03011804 2.3505056 0.0870125 0.06096667 0.1015000 0.04053333 3.970280e-01 1.032563e-31 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000151893 E001 2.9924970 0.0051800089 5.727409e-01 6.988223e-01 10 118674167 118674250 84 - 0.515 0.707 0.849
ENSG00000151893 E002 4.4179827 0.0037459069 4.448227e-01 5.863671e-01 10 118674251 118674460 210 - 0.634 0.854 0.893
ENSG00000151893 E003 1193.0884715 0.0085114871 1.119446e-11 2.312711e-10 10 118676411 118684555 8145 - 2.933 3.222 0.962
ENSG00000151893 E004 424.5090277 0.0001633989 1.700549e-10 2.885480e-09 10 118684556 118685958 1403 - 2.595 2.624 0.097
ENSG00000151893 E005 37.0287560 0.0190255146 1.740914e-01 2.934947e-01 10 118685959 118685982 24 - 1.555 1.549 -0.022
ENSG00000151893 E006 39.5481355 0.0096125839 5.158393e-02 1.119727e-01 10 118685983 118685996 14 - 1.589 1.566 -0.077
ENSG00000151893 E007 95.2287159 0.0011091422 2.245671e-06 1.727658e-05 10 118685997 118686168 172 - 1.977 1.918 -0.197
ENSG00000151893 E008 51.3149699 0.0004515009 2.420225e-04 1.141779e-03 10 118686598 118686641 44 - 1.713 1.658 -0.185
ENSG00000151893 E009 1.4175472 0.0954401652 4.485277e-01 5.898105e-01 10 118686642 118686781 140 - 0.389 0.307 -0.500
ENSG00000151893 E010 77.7835666 0.0015708599 3.451773e-05 2.021478e-04 10 118691265 118691403 139 - 1.889 1.836 -0.180
ENSG00000151893 E011 0.4396707 0.0320102526 2.305779e-01 3.631535e-01 10 118691404 118691565 162 - 0.210 0.000 -10.442
ENSG00000151893 E012 0.3332198 0.0346305174 6.616294e-01   10 118693702 118693749 48 - 0.082 0.180 1.309
ENSG00000151893 E013 2.4086607 0.0157513660 8.560714e-01 9.110477e-01 10 118694921 118695140 220 - 0.487 0.552 0.310
ENSG00000151893 E014 81.7917888 0.0017140777 1.183927e-06 9.680164e-06 10 118695141 118695230 90 - 1.918 1.830 -0.299
ENSG00000151893 E015 70.6774443 0.0114902760 1.697314e-03 6.274657e-03 10 118701306 118701408 103 - 1.856 1.763 -0.315
ENSG00000151893 E016 49.9402973 0.0057807026 4.349444e-03 1.412125e-02 10 118707492 118707587 96 - 1.697 1.643 -0.183
ENSG00000151893 E017 0.1515154 0.0439218960 9.274006e-01   10 118726278 118726385 108 - 0.082 0.000 -8.773
ENSG00000151893 E018 0.4418608 0.0262965553 2.298987e-01 3.623368e-01 10 118729175 118729294 120 - 0.210 0.000 -10.495
ENSG00000151893 E019 49.7463611 0.0115876983 1.048422e-04 5.443602e-04 10 118729295 118729397 103 - 1.727 1.555 -0.588
ENSG00000151893 E020 51.7099565 0.0183568883 1.257280e-05 8.165789e-05 10 118730284 118730410 127 - 1.764 1.503 -0.889
ENSG00000151893 E021 98.9565779 0.0116061976 1.069799e-07 1.080965e-06 10 118754396 118755249 854 - 2.035 1.807 -0.768