ENSG00000151458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000504087 ENSG00000151458 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKRD50 protein_coding protein_coding 3.375058 0.8311225 5.595134 0.09320922 0.1804428 2.736361 2.3188134 0.80809240 3.6623751 0.07554249 0.2757807 2.166378 0.80943750 0.9766 0.6577333 -0.3188667 0.0003651854 0.0003651854 FALSE TRUE
ENST00000515641 ENSG00000151458 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKRD50 protein_coding protein_coding 3.375058 0.8311225 5.595134 0.09320922 0.1804428 2.736361 0.4085861 0.00000000 0.8177825 0.00000000 0.1885906 6.371180 0.07112917 0.0000 0.1447000 0.1447000 0.0024549286 0.0003651854 FALSE TRUE
MSTRG.25432.1 ENSG00000151458 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKRD50 protein_coding   3.375058 0.8311225 5.595134 0.09320922 0.1804428 2.736361 0.6476582 0.02303012 1.1149761 0.02303012 0.3952094 5.089968 0.11943750 0.0234 0.1975667 0.1741667 0.0627616178 0.0003651854 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000151458 E001 0.0000000       4 124597030 124597075 46 -      
ENSG00000151458 E002 0.4762024 0.2386260361 4.576057e-01 5.979255e-01 4 124641878 124642014 137 - 0.104 0.253 1.550
ENSG00000151458 E003 1.0986217 0.0827071681 1.180193e-01 2.171015e-01 4 124651674 124651784 111 - 0.187 0.525 2.128
ENSG00000151458 E004 1.2500354 0.3327413107 2.885305e-02 6.970673e-02 4 124663285 124663350 66 - 0.147 0.682 3.237
ENSG00000151458 E005 66.4018667 0.0197747597 5.385771e-11 9.942682e-10 4 124664048 124665267 1220 - 1.558 2.092 1.803
ENSG00000151458 E006 148.4386362 0.0025556090 3.247165e-05 1.915390e-04 4 124665268 124667358 2091 - 2.011 2.210 0.669
ENSG00000151458 E007 28.1132858 0.0006924661 1.743004e-01 2.937810e-01 4 124667359 124667514 156 - 1.312 1.467 0.536
ENSG00000151458 E008 294.8163372 0.0004679250 1.299200e-08 1.568303e-07 4 124668984 124672534 3551 - 2.361 2.278 -0.274
ENSG00000151458 E009 27.3181032 0.0006957971 2.340191e-06 1.793745e-05 4 124678676 124678905 230 - 1.386 0.907 -1.719
ENSG00000151458 E010 110.1802879 0.0004439254 4.547248e-09 5.973253e-08 4 124710000 124711274 1275 - 1.955 1.724 -0.780
ENSG00000151458 E011 14.9697479 0.0129443139 1.244544e-02 3.460768e-02 4 124712458 124712732 275 - 1.127 0.814 -1.165