ENSG00000151065

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000280665 ENSG00000151065 HEK293_OSMI2_2hA HEK293_TMG_2hB DCP1B protein_coding protein_coding 10.14637 12.91596 9.899599 0.4500628 0.08424353 -0.383373 9.520358 12.60585 8.868656 0.4959779 0.3302248 -0.5068247 0.9330292 0.9757 0.8955 -0.0802 0.01824521 0.0003489709 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000151065 E001 32.4717872 0.0011914400 7.101841e-08 7.419746e-07 12 1946053 1946151 99 - 1.211 1.597 1.336
ENSG00000151065 E002 86.7190990 0.0003736427 2.843691e-09 3.876861e-08 12 1946152 1946286 135 - 1.747 1.991 0.823
ENSG00000151065 E003 146.8999102 0.0002666894 5.164098e-06 3.663582e-05 12 1949086 1949334 249 - 2.052 2.189 0.458
ENSG00000151065 E004 0.3666179 0.0323553802 7.336154e-01 8.247998e-01 12 1950036 1950377 342 - 0.150 0.099 -0.680
ENSG00000151065 E005 174.6299868 0.0003170958 6.538915e-03 2.002551e-02 12 1952416 1952776 361 - 2.172 2.246 0.246
ENSG00000151065 E006 216.4637663 0.0004262416 6.386159e-01 7.522097e-01 12 1952777 1953288 512 - 2.309 2.321 0.039
ENSG00000151065 E007 109.7057792 0.0003284333 1.376233e-01 2.447761e-01 12 1955432 1955560 129 - 2.057 2.008 -0.164
ENSG00000151065 E008 0.5953508 0.0307680639 6.354986e-01 7.497917e-01 12 1961316 1961395 80 - 0.261 0.179 -0.689
ENSG00000151065 E009 119.5491100 0.0003063720 3.064853e-03 1.045780e-02 12 1965558 1965693 136 - 2.121 2.028 -0.311
ENSG00000151065 E010 0.8126314 0.0142174364 6.006430e-02 1.267107e-01 12 1966080 1966141 62 - 0.423 0.099 -2.682
ENSG00000151065 E011 71.8975383 0.0004065389 2.529094e-03 8.849985e-03 12 1967844 1967910 67 - 1.917 1.793 -0.418
ENSG00000151065 E012 2.3260454 0.0061589129 6.663420e-03 2.034793e-02 12 1991121 1991246 126 - 0.743 0.307 -2.143
ENSG00000151065 E013 8.5313074 0.0381203554 3.502263e-01 4.944247e-01 12 1991456 1993263 1808 - 1.037 0.912 -0.465
ENSG00000151065 E014 85.7937527 0.0003787391 3.838532e-05 2.222353e-04 12 1993264 1993391 128 - 2.011 1.857 -0.517
ENSG00000151065 E015 53.1127469 0.0004503771 3.953628e-04 1.756609e-03 12 1997935 1997975 41 - 1.815 1.647 -0.568
ENSG00000151065 E016 0.3332198 0.0301889787 7.296916e-01   12 2004201 2004281 81 - 0.150 0.099 -0.683
ENSG00000151065 E017 54.9499150 0.0004393586 3.523866e-04 1.589717e-03 12 2004282 2004535 254 - 1.829 1.662 -0.563