ENSG00000150779

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000504148 ENSG00000150779 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8B protein_coding protein_coding 142.8202 228.2807 110.0581 19.8266 2.059087 -1.052476 63.99171 100.43633 55.14493 7.517367 16.416956 -0.8648629 0.4545250 0.4409000 0.4966333 0.05573333 0.9019706174 0.0001914578 FALSE  
ENST00000507614 ENSG00000150779 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8B protein_coding processed_transcript 142.8202 228.2807 110.0581 19.8266 2.059087 -1.052476 10.47861 11.33388 13.23931 2.730932 1.024367 0.2240034 0.0767250 0.0484000 0.1201333 0.07173333 0.0001914578 0.0001914578    
ENST00000541231 ENSG00000150779 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8B protein_coding protein_coding 142.8202 228.2807 110.0581 19.8266 2.059087 -1.052476 61.67593 109.01762 34.40926 7.930961 15.207768 -1.6634057 0.4190125 0.4786333 0.3171000 -0.16153333 0.6355588921 0.0001914578 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000150779 E001 5.615322 0.0028574927 1.319935e-10 2.284467e-09 11 112084800 112084816 17 - 1.209 0.392 -3.370
ENSG00000150779 E002 38.584091 0.0056728396 9.802259e-12 2.045164e-10 11 112084817 112085148 332 - 1.831 1.388 -1.509
ENSG00000150779 E003 2813.748612 0.0003210745 8.940682e-09 1.112137e-07 11 112085149 112085462 314 - 3.371 3.400 0.098
ENSG00000150779 E004 3.572871 0.0061523877 6.585969e-02 1.364776e-01 11 112085967 112085977 11 - 0.817 0.545 -1.148
ENSG00000150779 E005 254.007966 0.0093296646 3.472156e-12 7.802035e-11 11 112085978 112086310 333 - 2.577 2.235 -1.143
ENSG00000150779 E006 1522.453116 0.0018760903 1.314134e-01 2.361199e-01 11 112086640 112086798 159 - 3.112 3.128 0.054