ENSG00000150477

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000280020 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding protein_coding 4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.3806460 0.04505343 0.4128661 0.04505343 0.21131244 2.94129652 0.08472500 0.01743333 0.08020000 0.062766667 6.530304e-01 5.778127e-05 FALSE TRUE
ENST00000586501 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding protein_coding 4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.4792390 0.26874796 0.6296914 0.08244052 0.11435149 1.19841496 0.11835417 0.09390000 0.12053333 0.026633333 7.927291e-01 5.778127e-05 FALSE TRUE
ENST00000590617 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding nonsense_mediated_decay 4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.2079996 0.10361364 0.1046208 0.06484603 0.07401826 0.01273281 0.05082500 0.03393333 0.02013333 -0.013800000 8.502237e-01 5.778127e-05 FALSE TRUE
ENST00000592521 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding protein_coding 4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 1.6468951 1.93888111 2.1426362 0.13908002 0.10421310 0.14345844 0.42907500 0.68910000 0.40560000 -0.283500000 5.778127e-05 5.778127e-05 FALSE TRUE
MSTRG.15713.1 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding   4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.2496506 0.11760008 0.1970232 0.05194254 0.12993634 0.69816316 0.05938333 0.04380000 0.03576667 -0.008033333 8.750602e-01 5.778127e-05 TRUE TRUE
MSTRG.15713.2 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding   4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.3304754 0.00000000 0.5151226 0.00000000 0.28473449 5.71458248 0.06455000 0.00000000 0.09413333 0.094133333 2.770191e-01 5.778127e-05 FALSE TRUE
MSTRG.15713.8 ENSG00000150477 HEK293_OSMI2_2hA HEK293_TMG_2hB KIAA1328 protein_coding   4.114992 2.820637 5.294214 0.2296773 0.169044 0.9060122 0.2422299 0.19032207 0.4480754 0.07036882 0.22458283 1.19326383 0.05419167 0.06703333 0.08430000 0.017266667 1.000000e+00 5.778127e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000150477 E001 0.2944980 0.3163138006 6.090425e-01   18 36829098 36829105 8 + 0.163 0.000 -10.597
ENSG00000150477 E002 0.2944980 0.3163138006 6.090425e-01   18 36829106 36829126 21 + 0.163 0.000 -12.487
ENSG00000150477 E003 0.2944980 0.3163138006 6.090425e-01   18 36829127 36829128 2 + 0.163 0.000 -12.487
ENSG00000150477 E004 0.2944980 0.3163138006 6.090425e-01   18 36829129 36829130 2 + 0.163 0.000 -12.487
ENSG00000150477 E005 6.5877913 0.0032308747 3.701206e-02 8.549128e-02 18 36829131 36829196 66 + 0.762 0.994 0.891
ENSG00000150477 E006 1.0287806 0.0117756348 4.270757e-02 9.600955e-02 18 36829240 36829259 20 + 0.414 0.000 -14.582
ENSG00000150477 E007 5.3143527 0.0031472670 3.082632e-01 4.505303e-01 18 36829260 36829439 180 + 0.840 0.675 -0.665
ENSG00000150477 E008 4.0674404 0.0038868829 2.720261e-04 1.265956e-03 18 36832674 36833388 715 + 0.840 0.166 -3.665
ENSG00000150477 E009 1.8476529 0.0086079953 6.239969e-01 7.404593e-01 18 36834203 36834319 117 + 0.484 0.380 -0.551
ENSG00000150477 E010 13.9875110 0.0014009540 8.408413e-02 1.659425e-01 18 36834320 36834355 36 + 1.108 1.238 0.464
ENSG00000150477 E011 29.5766780 0.0007267021 1.578116e-02 4.224162e-02 18 36835234 36835376 143 + 1.419 1.544 0.430
ENSG00000150477 E012 27.6820872 0.0019735195 5.320732e-03 1.680583e-02 18 36844208 36844302 95 + 1.380 1.538 0.545
ENSG00000150477 E013 29.0618437 0.0013860884 3.238321e-03 1.096811e-02 18 36885557 36885672 116 + 1.400 1.561 0.554
ENSG00000150477 E014 31.1838365 0.0007387151 9.954500e-07 8.262213e-06 18 36885673 36886404 732 + 1.375 1.642 0.914
ENSG00000150477 E015 0.0000000       18 36923905 36923910 6 +      
ENSG00000150477 E016 0.0000000       18 36923911 36923939 29 +      
ENSG00000150477 E017 0.0000000       18 36923940 36923957 18 +      
ENSG00000150477 E018 0.0000000       18 36923958 36923961 4 +      
ENSG00000150477 E019 0.0000000       18 36924912 36924915 4 +      
ENSG00000150477 E020 0.0000000       18 36924916 36925042 127 +      
ENSG00000150477 E021 0.1472490 0.0428174405 1.000000e+00   18 36946249 36946517 269 + 0.089 0.000 -12.101
ENSG00000150477 E022 0.2987644 0.0272282447 5.103052e-01   18 36954431 36954504 74 + 0.163 0.000 -13.059
ENSG00000150477 E023 0.3030308 0.3995692865 6.242238e-01   18 36955815 36955852 38 + 0.163 0.000 -12.401
ENSG00000150477 E024 9.1921682 0.0154211520 5.271550e-01 6.602936e-01 18 36959308 36959435 128 + 1.032 0.927 -0.390
ENSG00000150477 E025 0.2987644 0.0272282447 5.103052e-01   18 36975927 36976012 86 + 0.163 0.000 -13.059
ENSG00000150477 E026 0.0000000       18 37066866 37066889 24 +      
ENSG00000150477 E027 18.8388711 0.0197119549 2.519562e-01 3.882730e-01 18 37066890 37067339 450 + 1.336 1.187 -0.521
ENSG00000150477 E028 11.0738150 0.0016033845 7.943308e-02 1.586342e-01 18 37067340 37067545 206 + 1.138 0.927 -0.773
ENSG00000150477 E029 0.1472490 0.0428174405 1.000000e+00   18 37074752 37075648 897 + 0.089 0.000 -12.101
ENSG00000150477 E030 2.5644425 0.0298461264 9.864967e-01 9.956200e-01 18 37084125 37084232 108 + 0.544 0.523 -0.103
ENSG00000150477 E031 0.2214452 0.0379566945 2.226645e-01   18 37084233 37084425 193 + 0.000 0.166 12.458
ENSG00000150477 E032 11.0260715 0.0373470472 5.541089e-02 1.186901e-01 18 37160200 37160381 182 + 1.152 0.852 -1.110
ENSG00000150477 E033 12.1688858 0.0524669625 1.052661e-01 1.984985e-01 18 37172973 37173081 109 + 1.184 0.909 -1.007
ENSG00000150477 E034 0.0000000       18 37193591 37193694 104 +      
ENSG00000150477 E035 11.5612508 0.0066084451 2.276470e-01 3.595898e-01 18 37222017 37222117 101 + 1.130 0.973 -0.572
ENSG00000150477 E036 72.5078293 0.0048966179 7.048696e-05 3.824696e-04 18 37222118 37225283 3166 + 1.917 1.696 -0.745
ENSG00000150477 E037 1.8884630 0.0086091571 1.914335e-01 3.155447e-01 18 37225284 37225334 51 + 0.374 0.579 1.033
ENSG00000150477 E038 1.0589695 0.0500357369 3.224311e-01 4.657325e-01 18 37229569 37229578 10 + 0.373 0.166 -1.548
ENSG00000150477 E039 2.2402079 0.0285002399 6.958852e-01 7.963645e-01 18 37229579 37229659 81 + 0.544 0.456 -0.430
ENSG00000150477 E040 5.3705805 0.0036338901 1.314359e-01 2.361433e-01 18 37229660 37231373 1714 + 0.867 0.629 -0.965
ENSG00000150477 E041 3.9887968 0.0042734322 7.399661e-01 8.295079e-01 18 37231374 37231562 189 + 0.687 0.715 0.116
ENSG00000150477 E042 0.5149111 0.0199939831 2.162128e-01 3.458539e-01 18 37231563 37231659 97 + 0.089 0.286 2.035
ENSG00000150477 E043 3.6953138 0.0113518216 3.062298e-02 7.318480e-02 18 37231660 37232172 513 + 0.515 0.819 1.299