ENSG00000149743

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000317459 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding protein_coding 31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 10.4419672 19.5266023 5.4770737 1.1871947 0.85312895 -1.83207072 0.31789167 0.37333333 0.28286667 -0.09046667 3.472957e-01 3.047082e-28 FALSE TRUE
ENST00000394547 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding protein_coding 31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 2.2773874 2.0204683 2.5791759 1.0132802 0.33857500 0.35068043 0.08150000 0.04066667 0.13226667 0.09160000 4.738191e-01 3.047082e-28 FALSE TRUE
ENST00000541928 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding retained_intron 31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 1.4106147 0.6839405 0.8701067 0.2645755 0.08021382 0.34286653 0.05591667 0.01296667 0.04466667 0.03170000 2.502513e-02 3.047082e-28 TRUE FALSE
ENST00000546133 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding protein_coding 31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 1.8221780 1.6435752 1.5984405 0.4371101 0.13681886 -0.03992611 0.06250417 0.03196667 0.08203333 0.05006667 4.475153e-02 3.047082e-28 FALSE TRUE
MSTRG.5663.17 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding   31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 1.9261401 5.3958963 0.3950771 0.3315117 0.39507713 -3.73826527 0.04947500 0.10413333 0.02110000 -0.08303333 1.029422e-01 3.047082e-28 FALSE TRUE
MSTRG.5663.21 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding   31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 4.4422557 9.5939276 0.8661422 1.4759253 0.48544775 -3.45438755 0.12384167 0.18160000 0.04340000 -0.13820000 2.847831e-01 3.047082e-28   FALSE
MSTRG.5663.7 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding   31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 0.9267226 0.0000000 2.0889338 0.0000000 0.27167524 7.71351285 0.03485417 0.00000000 0.10820000 0.10820000 3.047082e-28 3.047082e-28 FALSE TRUE
MSTRG.5663.9 ENSG00000149743 HEK293_OSMI2_2hA HEK293_TMG_2hB TRPT1 protein_coding   31.42575 52.26343 19.42195 2.804827 0.5322565 -1.427647 1.3744165 4.0976128 0.4626629 0.1835278 0.24656143 -3.11941672 0.04190000 0.07853333 0.02450000 -0.05403333 3.479348e-01 3.047082e-28 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149743 E001 0.4513240 0.1565542105 1.551352e-01 2.685732e-01 11 64223226 64223431 206 - 0.330 0.074 -2.623
ENSG00000149743 E002 0.6330284 0.0202453069 2.804746e-01 4.203286e-01 11 64223432 64223798 367 - 0.330 0.138 -1.609
ENSG00000149743 E003 0.1482932 0.0412106625 1.000000e+00   11 64223799 64223799 1 - 0.000 0.074 10.560
ENSG00000149743 E004 0.2965864 0.2033497018 8.141973e-01   11 64223800 64223815 16 - 0.000 0.135 10.900
ENSG00000149743 E005 1.3660607 0.0176480339 6.862097e-02 1.410747e-01 11 64223816 64223829 14 - 0.000 0.396 13.723
ENSG00000149743 E006 1.8840923 0.0651006193 3.588324e-02 8.335751e-02 11 64223830 64223832 3 - 0.000 0.482 13.764
ENSG00000149743 E007 2.3957942 0.0095849339 9.917601e-03 2.855801e-02 11 64223833 64223841 9 - 0.000 0.557 14.574
ENSG00000149743 E008 3.0955302 0.0090166483 2.972161e-03 1.018020e-02 11 64223842 64223852 11 - 0.000 0.638 14.908
ENSG00000149743 E009 3.3169754 0.0057100556 2.082698e-03 7.480111e-03 11 64223853 64223855 3 - 0.000 0.657 15.021
ENSG00000149743 E010 4.3435884 0.0040566092 5.120721e-03 1.625784e-02 11 64223856 64223876 21 - 0.195 0.738 2.977
ENSG00000149743 E011 28.5865304 0.0042789467 1.607666e-01 2.761189e-01 11 64223877 64223887 11 - 1.295 1.417 0.421
ENSG00000149743 E012 155.6312533 0.0003770412 7.386695e-02 1.496911e-01 11 64223888 64223967 80 - 2.059 2.122 0.211
ENSG00000149743 E013 11.6991842 0.0028535832 9.864839e-02 1.884259e-01 11 64223968 64224099 132 - 1.148 0.973 -0.637
ENSG00000149743 E014 162.0244059 0.0022855105 6.390782e-01 7.525481e-01 11 64224100 64224162 63 - 2.139 2.125 -0.050
ENSG00000149743 E015 136.0082146 0.0003599299 8.769723e-01 9.250080e-01 11 64224163 64224202 40 - 2.046 2.053 0.024
ENSG00000149743 E016 101.4655397 0.0003220113 9.920602e-01 9.991396e-01 11 64224203 64224210 8 - 1.924 1.925 0.004
ENSG00000149743 E017 17.1163774 0.0016640236 2.545695e-01 3.912849e-01 11 64224211 64224213 3 - 1.256 1.153 -0.364
ENSG00000149743 E018 12.0992652 0.0109757924 3.284380e-03 1.110485e-02 11 64224214 64224284 71 - 1.256 0.939 -1.149
ENSG00000149743 E019 237.5823116 0.0001908569 4.107621e-01 5.543569e-01 11 64224285 64224341 57 - 2.305 2.286 -0.066
ENSG00000149743 E020 21.4739679 0.0024944000 8.469991e-05 4.502917e-04 11 64224342 64224491 150 - 1.485 1.171 -1.094
ENSG00000149743 E021 9.8576269 0.0017969623 7.570348e-03 2.269527e-02 11 64224492 64224542 51 - 1.165 0.877 -1.062
ENSG00000149743 E022 308.4362594 0.0002648525 9.998967e-01 1.000000e+00 11 64224543 64224653 111 - 2.400 2.402 0.006
ENSG00000149743 E023 232.2686912 0.0002981199 8.541884e-02 1.680811e-01 11 64224654 64224717 64 - 2.238 2.287 0.164
ENSG00000149743 E024 9.2674693 0.0052595611 6.059608e-01 7.262475e-01 11 64224759 64224794 36 - 0.979 0.918 -0.228
ENSG00000149743 E025 8.9720262 0.0217873955 8.771165e-01 9.250932e-01 11 64224795 64224800 6 - 0.893 0.920 0.101
ENSG00000149743 E026 215.5658593 0.0002053498 1.286952e-01 2.323363e-01 11 64224801 64224970 170 - 2.212 2.256 0.149
ENSG00000149743 E027 43.5389304 0.0058319418 6.248689e-06 4.351869e-05 11 64224971 64225498 528 - 1.770 1.476 -1.002
ENSG00000149743 E028 189.4368725 0.0004130165 6.554041e-01 7.652964e-01 11 64225499 64225580 82 - 2.177 2.193 0.052
ENSG00000149743 E029 116.7308968 0.0059368371 8.959948e-02 1.745148e-01 11 64225786 64225869 84 - 1.909 2.001 0.312
ENSG00000149743 E030 22.2267265 0.0079469991 1.766545e-01 2.968992e-01 11 64225870 64226049 180 - 1.364 1.244 -0.420
ENSG00000149743 E031 173.0359330 0.0049447578 7.063947e-01 8.045423e-01 11 64226050 64226190 141 - 2.162 2.149 -0.044
ENSG00000149743 E032 19.4444147 0.0177409678 5.118441e-01 6.467809e-01 11 64226191 64226254 64 - 1.270 1.203 -0.235