ENSG00000149658

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370339 ENSG00000149658 HEK293_OSMI2_2hA HEK293_TMG_2hB YTHDF1 protein_coding protein_coding 37.53902 43.36839 46.96595 2.558558 1.131073 0.1149457 32.759748 40.223899 38.690460 2.68678180 1.1222648 -0.05606086 0.8746875 0.92670000 0.8236333 -0.10306667 1.747968e-08 1.204416e-14 FALSE TRUE
MSTRG.20970.2 ENSG00000149658 HEK293_OSMI2_2hA HEK293_TMG_2hB YTHDF1 protein_coding   37.53902 43.36839 46.96595 2.558558 1.131073 0.1149457 2.735228 1.267645 5.407866 0.04315627 0.2477505 2.08423760 0.0715000 0.02946667 0.1154333 0.08596667 1.204416e-14 1.204416e-14 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149658 E001 33.43264 0.0034995619 2.671061e-02 6.538967e-02 20 63195429 63195455 27 - 1.447 1.598 0.518
ENSG00000149658 E002 361.65090 0.0031241323 2.594782e-07 2.431727e-06 20 63195456 63195586 131 - 2.452 2.621 0.562
ENSG00000149658 E003 664.40625 0.0051552149 3.329274e-07 3.051088e-06 20 63195587 63195770 184 - 2.706 2.886 0.598
ENSG00000149658 E004 1106.56466 0.0008009133 1.588016e-41 6.053216e-39 20 63195771 63196366 596 - 2.912 3.116 0.679
ENSG00000149658 E005 282.21975 0.0001841965 9.324070e-12 1.952921e-10 20 63196367 63196454 88 - 2.358 2.507 0.495
ENSG00000149658 E006 610.18999 0.0014970935 5.420551e-01 6.729133e-01 20 63196455 63196734 280 - 2.777 2.785 0.029
ENSG00000149658 E007 1751.23955 0.0026932567 1.187003e-10 2.069901e-09 20 63202287 63203799 1513 - 3.308 3.179 -0.428
ENSG00000149658 E008 145.60336 0.0002969883 3.572749e-11 6.790875e-10 20 63203800 63203807 8 - 2.265 2.069 -0.656
ENSG00000149658 E009 226.87748 0.0003260644 1.578113e-27 2.157061e-25 20 63213864 63213943 80 - 2.487 2.221 -0.885
ENSG00000149658 E010 117.64932 0.0003937640 5.082601e-34 1.160101e-31 20 63215577 63215601 25 - 2.259 1.848 -1.380
ENSG00000149658 E011 52.78647 0.0005582431 3.901793e-21 2.849588e-19 20 63215602 63215865 264 - 1.934 1.460 -1.607
ENSG00000149658 E012 120.53551 0.0120460671 1.408492e-16 5.952143e-15 20 63215866 63216139 274 - 2.308 1.774 -1.793