ENSG00000149657

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000279068 ENSG00000149657 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM14B protein_coding protein_coding 32.35271 34.32264 28.0513 0.6645745 0.3374813 -0.2909987 11.418881 13.3818813 8.434077 1.6998145 0.4109232 -0.66534706 0.35022917 0.38833333 0.3006333 -0.08770000 3.204287e-01 1.817396e-06 FALSE TRUE
ENST00000370915 ENSG00000149657 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM14B protein_coding protein_coding 32.35271 34.32264 28.0513 0.6645745 0.3374813 -0.2909987 2.580129 0.9941559 3.067242 0.2118233 0.1164665 1.61565468 0.08111250 0.02883333 0.1094000 0.08056667 1.817396e-06 1.817396e-06 FALSE TRUE
MSTRG.20946.1 ENSG00000149657 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM14B protein_coding   32.35271 34.32264 28.0513 0.6645745 0.3374813 -0.2909987 10.913897 13.8211707 9.324105 0.2063895 0.6159109 -0.56733961 0.33586667 0.40300000 0.3328000 -0.07020000 2.311887e-01 1.817396e-06 FALSE TRUE
MSTRG.20946.3 ENSG00000149657 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM14B protein_coding   32.35271 34.32264 28.0513 0.6645745 0.3374813 -0.2909987 3.322096 3.5177953 3.385740 0.8066627 0.5311468 -0.05504077 0.10404583 0.10336667 0.1203000 0.01693333 8.174152e-01 1.817396e-06 FALSE TRUE
MSTRG.20946.6 ENSG00000149657 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM14B protein_coding   32.35271 34.32264 28.0513 0.6645745 0.3374813 -0.2909987 2.687505 1.7943630 2.251706 0.3772035 0.1200451 0.32592171 0.08250417 0.05260000 0.0802000 0.02760000 3.159191e-01 1.817396e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149657 E001 3.4142866 0.2276279418 1.861781e-02 4.849432e-02 20 62122461 62122463 3 + 0.884 0.373 -2.288
ENSG00000149657 E002 5.1052378 0.0586841064 1.083157e-02 3.076197e-02 20 62122464 62122474 11 + 0.983 0.571 -1.662
ENSG00000149657 E003 6.3575519 0.0067341022 9.254399e-04 3.704086e-03 20 62122475 62122481 7 + 1.064 0.654 -1.592
ENSG00000149657 E004 7.0939108 0.0074353778 7.719510e-05 4.147819e-04 20 62122482 62122486 5 + 1.132 0.654 -1.837
ENSG00000149657 E005 15.7269993 0.0993691758 6.389769e-05 3.505306e-04 20 62122487 62122526 40 + 1.519 0.810 -2.553
ENSG00000149657 E006 149.7573615 0.0008297261 2.708442e-18 1.412737e-16 20 62122527 62122793 267 + 2.311 2.048 -0.879
ENSG00000149657 E007 0.9545818 0.0139726076 2.001686e-01 3.264625e-01 20 62123152 62123414 263 + 0.410 0.188 -1.538
ENSG00000149657 E008 239.9306599 0.0005365188 5.065214e-04 2.184709e-03 20 62124617 62124780 164 + 2.412 2.334 -0.262
ENSG00000149657 E009 11.0148621 0.0015859670 7.360666e-04 3.029056e-03 20 62126226 62126303 78 + 1.242 0.919 -1.176
ENSG00000149657 E010 233.3384159 0.0001687926 3.374686e-01 4.813607e-01 20 62126304 62126439 136 + 2.363 2.348 -0.050
ENSG00000149657 E011 125.4962419 0.0002974390 1.914753e-01 3.155862e-01 20 62127585 62127701 117 + 2.106 2.072 -0.116
ENSG00000149657 E012 29.8704149 0.0015486651 4.240286e-12 9.381169e-11 20 62127702 62128030 329 + 1.696 1.249 -1.537
ENSG00000149657 E013 30.3449073 0.0007076119 1.589850e-01 2.737713e-01 20 62128925 62129032 108 + 1.526 1.443 -0.284
ENSG00000149657 E014 249.4430849 0.0006916345 9.830440e-01 9.934638e-01 20 62129785 62129952 168 + 2.379 2.387 0.025
ENSG00000149657 E015 224.1072534 0.0002685696 1.249628e-02 3.472508e-02 20 62130219 62130296 78 + 2.371 2.318 -0.176
ENSG00000149657 E016 355.0830289 0.0002013028 7.818276e-02 1.565427e-01 20 62130530 62130691 162 + 2.552 2.526 -0.087
ENSG00000149657 E017 361.2944795 0.0001563926 1.486060e-01 2.597757e-01 20 62131356 62131506 151 + 2.521 2.556 0.116
ENSG00000149657 E018 439.5216022 0.0001701171 1.280235e-04 6.501628e-04 20 62133290 62133475 186 + 2.580 2.654 0.247
ENSG00000149657 E019 1123.8876779 0.0005797481 7.150062e-16 2.753726e-14 20 62134163 62135520 1358 + 2.962 3.071 0.360