ENSG00000149346

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334534 ENSG00000149346 HEK293_OSMI2_2hA HEK293_TMG_2hB SLX4IP protein_coding protein_coding 2.303165 2.149826 2.067447 0.3082752 0.109915 -0.05610348 1.3873032 0.8989267 1.5782184 0.03388656 0.06501587 0.8051734 0.6144750 0.4300333 0.7664000 0.3363667 0.001200974 0.001200974 FALSE TRUE
MSTRG.20345.2 ENSG00000149346 HEK293_OSMI2_2hA HEK293_TMG_2hB SLX4IP protein_coding   2.303165 2.149826 2.067447 0.3082752 0.109915 -0.05610348 0.6964589 1.0855754 0.2724274 0.12159964 0.18534946 -1.9557367 0.2863875 0.5093000 0.1232667 -0.3860333 0.069579159 0.001200974 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149346 E001 0.3030308 0.3360209334 3.760407e-01   20 10435259 10435282 24 + 0.216 0.000 -10.604
ENSG00000149346 E002 0.6266857 0.0172671820 8.100990e-01 8.794739e-01 20 10435283 10435304 22 + 0.216 0.220 0.033
ENSG00000149346 E003 3.0448215 0.0311792078 4.981489e-01 6.346609e-01 20 10435305 10435336 32 + 0.591 0.631 0.177
ENSG00000149346 E004 13.5255718 0.0213675659 5.755096e-01 7.011032e-01 20 10435337 10435453 117 + 1.241 1.093 -0.527
ENSG00000149346 E005 11.8114252 0.0085768567 6.392947e-01 7.527020e-01 20 10458176 10458231 56 + 1.181 1.048 -0.478
ENSG00000149346 E006 13.1576938 0.0014565010 1.456403e-01 2.557195e-01 20 10556231 10556320 90 + 1.256 1.036 -0.787
ENSG00000149346 E007 12.9249659 0.0174171782 6.609943e-01 7.695622e-01 20 10560700 10560820 121 + 1.171 1.128 -0.151
ENSG00000149346 E008 9.1261084 0.0166618127 9.169258e-01 9.515373e-01 20 10598675 10598752 78 + 1.053 0.978 -0.274
ENSG00000149346 E009 10.1051296 0.0017920087 8.324449e-01 8.950178e-01 20 10601731 10601819 89 + 1.088 1.023 -0.239
ENSG00000149346 E010 4.3614935 0.1064644356 2.712080e-01 4.099246e-01 20 10621314 10621414 101 + 0.870 0.561 -1.281
ENSG00000149346 E011 96.8428249 0.0312285738 1.805425e-05 1.131396e-04 20 10622659 10626310 3652 + 2.161 1.761 -1.342
ENSG00000149346 E012 80.8410422 0.0005789708 2.668787e-09 3.658155e-08 20 10626311 10627791 1481 + 1.852 1.961 0.368
ENSG00000149346 E013 12.2765892 0.0229450813 1.258238e-03 4.837237e-03 20 10627792 10627802 11 + 0.941 1.239 1.079
ENSG00000149346 E014 23.9903448 0.0009283193 2.008664e-10 3.364890e-09 20 10627803 10628030 228 + 1.180 1.534 1.231
ENSG00000149346 E015 0.2924217 0.0276425529 2.830893e-01   20 10636296 10636438 143 + 0.216 0.000 -13.273
ENSG00000149346 E016 0.8973375 0.0553964362 1.728832e-01 2.919401e-01 20 10636543 10636829 287 + 0.417 0.123 -2.294