ENSG00000149313

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000278618 ENSG00000149313 HEK293_OSMI2_2hA HEK293_TMG_2hB AASDHPPT protein_coding protein_coding 43.17336 14.99552 71.66832 2.072735 1.663435 2.256043 31.230415 12.8675032 53.410316 2.1303861 1.9553502 2.052536 0.7436208 0.85216667 0.7448333 -0.10733333 0.01558888 0.01558888 FALSE TRUE
ENST00000524411 ENSG00000149313 HEK293_OSMI2_2hA HEK293_TMG_2hB AASDHPPT protein_coding protein_coding 43.17336 14.99552 71.66832 2.072735 1.663435 2.256043 6.519841 0.5166669 8.128286 0.5166669 0.5738786 3.949762 0.1356042 0.04036667 0.1131667 0.07280000 0.16918186 0.01558888 FALSE FALSE
ENST00000525660 ENSG00000149313 HEK293_OSMI2_2hA HEK293_TMG_2hB AASDHPPT protein_coding nonsense_mediated_decay 43.17336 14.99552 71.66832 2.072735 1.663435 2.256043 5.227527 1.5823804 9.786729 0.5137104 0.9039039 2.621115 0.1158750 0.10526667 0.1372333 0.03196667 0.69852956 0.01558888 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149313 E001 0.00000       11 106075501 106075576 76 +      
ENSG00000149313 E002 0.00000       11 106076242 106076288 47 +      
ENSG00000149313 E003 29.28572 0.0159777587 0.2730671657 0.412050233 11 106077658 106077661 4 + 1.407 1.351 -0.195
ENSG00000149313 E004 306.86535 0.0037031431 0.0004709154 0.002048860 11 106077662 106077893 232 + 2.417 2.340 -0.259
ENSG00000149313 E005 408.11387 0.0021253917 0.0107471108 0.030560594 11 106079467 106079692 226 + 2.530 2.511 -0.063
ENSG00000149313 E006 289.19207 0.0048631382 0.8146466630 0.882569990 11 106090557 106090678 122 + 2.363 2.423 0.198
ENSG00000149313 E007 402.54222 0.0039728550 0.2822255330 0.422201330 11 106091316 106091466 151 + 2.517 2.530 0.044
ENSG00000149313 E008 212.14742 0.0006542195 0.0468564633 0.103576496 11 106091467 106091477 11 + 2.243 2.241 -0.008
ENSG00000149313 E009 15.97541 0.0011818633 0.0010355393 0.004081128 11 106091478 106094582 3105 + 1.197 0.858 -1.244
ENSG00000149313 E010 311.67478 0.0001762522 0.0037432892 0.012419960 11 106094583 106094654 72 + 2.412 2.403 -0.030
ENSG00000149313 E011 1775.08196 0.0091869118 0.0195091379 0.050431838 11 106096743 106098699 1957 + 3.134 3.253 0.394