ENSG00000149308

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000278612 ENSG00000149308 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAT protein_coding protein_coding 6.278588 1.476433 9.528035 0.230511 0.2545362 2.681838 3.2691800 1.0883013 5.3182841 0.1159076 0.2053216 2.2783972 0.56012083 0.7522667 0.55803333 -0.19423333 2.661588e-02 2.350795e-09 FALSE TRUE
ENST00000530859 ENSG00000149308 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAT protein_coding retained_intron 6.278588 1.476433 9.528035 0.230511 0.2545362 2.681838 0.4027380 0.3131516 0.1525630 0.1566262 0.1525630 -0.9912123 0.13239583 0.1877333 0.01600000 -0.17173333 2.739501e-01 2.350795e-09 FALSE TRUE
MSTRG.6268.3 ENSG00000149308 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAT protein_coding   6.278588 1.476433 9.528035 0.230511 0.2545362 2.681838 1.5807894 0.0000000 2.7330460 0.0000000 0.3282863 8.0996350 0.15641667 0.0000000 0.28683333 0.28683333 2.350795e-09 2.350795e-09 FALSE TRUE
MSTRG.6268.6 ENSG00000149308 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAT protein_coding   6.278588 1.476433 9.528035 0.230511 0.2545362 2.681838 0.1987337 0.0000000 0.8143964 0.0000000 0.3837013 6.3652663 0.01829167 0.0000000 0.08626667 0.08626667 5.080998e-02 2.350795e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149308 E001 0.2955422 0.0290785164 1.728584e-01   11 108151458 108151507 50 - 0.052 0.262 2.693
ENSG00000149308 E002 0.5149111 0.0199878858 3.132402e-02 7.452923e-02 11 108151508 108151624 117 - 0.052 0.424 3.696
ENSG00000149308 E003 0.0000000       11 108151625 108151628 4 -      
ENSG00000149308 E004 0.4418608 0.0246589134 1.000000e+00 1.000000e+00 11 108157211 108157214 4 - 0.141 0.000 -9.778
ENSG00000149308 E005 110.6461431 0.0004710948 4.841783e-30 8.182527e-28 11 108157215 108158637 1423 - 1.811 2.228 1.402
ENSG00000149308 E006 34.5184395 0.0006219821 2.888285e-02 6.976627e-02 11 108158638 108158792 155 - 1.387 1.544 0.538
ENSG00000149308 E007 38.3120508 0.0006787922 2.251494e-02 5.675229e-02 11 108158793 108158919 127 - 1.426 1.583 0.537
ENSG00000149308 E008 41.7425341 0.0031822491 4.076594e-03 1.335810e-02 11 108158920 108159019 100 - 1.455 1.653 0.678
ENSG00000149308 E009 72.7975947 0.0009471909 5.486497e-05 3.060120e-04 11 108160880 108161183 304 - 1.691 1.893 0.681
ENSG00000149308 E010 37.0746738 0.0082438174 2.817763e-02 6.836355e-02 11 108161184 108161320 137 - 1.413 1.584 0.587
ENSG00000149308 E011 107.6570155 0.0003423825 4.454002e-01 5.869404e-01 11 108161321 108162014 694 - 1.893 1.919 0.090
ENSG00000149308 E012 0.5838113 0.3978175525 4.750448e-01 6.138073e-01 11 108162015 108162035 21 - 0.140 0.258 1.088
ENSG00000149308 E013 24.9226354 0.0012007748 1.055727e-01 1.989676e-01 11 108162120 108162180 61 - 1.305 1.128 -0.626
ENSG00000149308 E014 36.8084475 0.0089875450 9.350209e-01 9.630895e-01 11 108169744 108169852 109 - 1.441 1.442 0.003
ENSG00000149308 E015 41.7138646 0.0005559664 9.670114e-01 9.833216e-01 11 108169928 108170043 116 - 1.494 1.489 -0.016
ENSG00000149308 E016 0.1472490 0.0426238150 8.350164e-01   11 108170044 108170201 158 - 0.052 0.000 -8.195
ENSG00000149308 E017 0.7374030 0.0156140475 6.570872e-01 7.665269e-01 11 108171132 108172198 1067 - 0.179 0.262 0.694
ENSG00000149308 E018 129.8249409 0.0008770155 5.965685e-03 1.851633e-02 11 108172199 108172954 756 - 1.999 1.864 -0.454
ENSG00000149308 E019 121.6390254 0.0004719142 1.353526e-04 6.827526e-04 11 108172955 108173851 897 - 1.976 1.783 -0.650
ENSG00000149308 E020 32.2219654 0.0047213941 4.342886e-02 9.734712e-02 11 108176246 108176374 129 - 1.412 1.203 -0.730
ENSG00000149308 E021 24.8814741 0.0070374498 8.239396e-02 1.632890e-01 11 108176994 108177090 97 - 1.305 1.101 -0.723
ENSG00000149308 E022 24.8425394 0.0007949825 1.568013e-02 4.202294e-02 11 108185232 108185319 88 - 1.313 1.039 -0.977
ENSG00000149308 E023 26.1651075 0.0007384614 1.777216e-01 2.982577e-01 11 108185403 108185494 92 - 1.321 1.178 -0.502
ENSG00000149308 E024 31.8576186 0.0219546036 1.046508e-01 1.975882e-01 11 108186482 108186569 88 - 1.409 1.204 -0.714
ENSG00000149308 E025 28.8696861 0.0176695364 1.970093e-01 3.224795e-01 11 108188098 108188179 82 - 1.361 1.205 -0.547
ENSG00000149308 E026 43.9935363 0.0462956922 2.065892e-03 7.428622e-03 11 108189106 108189330 225 - 1.560 1.140 -1.465
ENSG00000149308 E027 0.2966881 0.0290785164 1.000000e+00   11 108189331 108189337 7 - 0.099 0.000 -9.208
ENSG00000149308 E028 19.5429669 0.0012331575 1.118607e-03 4.365636e-03 11 108190460 108190500 41 - 1.227 0.776 -1.673
ENSG00000149308 E029 23.5465541 0.0129103661 2.545737e-03 8.899837e-03 11 108192118 108192190 73 - 1.302 0.884 -1.517
ENSG00000149308 E030 23.9523888 0.0056780805 3.337071e-04 1.515190e-03 11 108193957 108194017 61 - 1.313 0.833 -1.752
ENSG00000149308 E031 29.5238963 0.0039765051 6.588701e-06 4.564672e-05 11 108197302 108197420 119 - 1.405 0.833 -2.073
ENSG00000149308 E032 0.7406253 0.0155137923 5.714646e-01 6.977504e-01 11 108204393 108204566 174 - 0.215 0.000 -10.482
ENSG00000149308 E033 1.2544922 0.0104140164 1.000000e+00 1.000000e+00 11 108208421 108208584 164 - 0.278 0.262 -0.110
ENSG00000149308 E034 18.5109786 0.0013575919 2.064163e-03 7.424099e-03 11 108222500 108222642 143 - 1.206 0.776 -1.602