ENSG00000149269

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356341 ENSG00000149269 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK1 protein_coding protein_coding 18.75279 23.94035 18.40419 1.563197 0.8517595 -0.379229 9.882579 16.3114044 8.146986 0.6905828 0.29447372 -1.0006571 0.51069167 0.68336667 0.44353333 -0.23983333 2.776342e-10 2.776342e-10 FALSE TRUE
ENST00000528203 ENSG00000149269 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK1 protein_coding protein_coding 18.75279 23.94035 18.40419 1.563197 0.8517595 -0.379229 1.115743 1.3586420 1.515412 0.1005675 0.07549593 0.1564543 0.05894167 0.05716667 0.08266667 0.02550000 1.869391e-01 2.776342e-10 FALSE TRUE
ENST00000530617 ENSG00000149269 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK1 protein_coding protein_coding 18.75279 23.94035 18.40419 1.563197 0.8517595 -0.379229 2.540027 4.1552858 1.834369 0.3774450 0.07892421 -1.1752880 0.13083750 0.17406667 0.10046667 -0.07360000 1.127686e-02 2.776342e-10 FALSE TRUE
MSTRG.6033.3 ENSG00000149269 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK1 protein_coding   18.75279 23.94035 18.40419 1.563197 0.8517595 -0.379229 1.775168 0.3576138 2.662275 0.3576138 0.24237745 2.8618055 0.10460000 0.01336667 0.14643333 0.13306667 3.588811e-02 2.776342e-10 FALSE TRUE
MSTRG.6033.4 ENSG00000149269 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK1 protein_coding   18.75279 23.94035 18.40419 1.563197 0.8517595 -0.379229 1.243500 0.7092985 1.823147 0.2914370 0.93613497 1.3496597 0.06902917 0.02836667 0.09503333 0.06666667 8.516402e-01 2.776342e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149269 E001 407.4284578 0.0019567169 8.306118e-13 2.066679e-11 11 77322017 77322638 622 - 2.443 2.646 0.674
ENSG00000149269 E002 284.7320402 0.0003131643 1.019803e-12 2.500954e-11 11 77322639 77322944 306 - 2.312 2.484 0.574
ENSG00000149269 E003 55.9325117 0.0009583962 3.510547e-05 2.052029e-04 11 77322945 77322945 1 - 1.568 1.793 0.762
ENSG00000149269 E004 58.6969907 0.0004687315 5.499918e-05 3.066754e-04 11 77322946 77322947 2 - 1.601 1.810 0.708
ENSG00000149269 E005 105.1925165 0.0003231751 1.438298e-07 1.417401e-06 11 77322948 77323023 76 - 1.856 2.060 0.688
ENSG00000149269 E006 173.5770011 0.0002255171 3.365071e-03 1.133660e-02 11 77323024 77323154 131 - 2.157 2.245 0.294
ENSG00000149269 E007 142.6072358 0.0012967965 2.073485e-01 3.353214e-01 11 77323155 77323210 56 - 2.102 2.150 0.161
ENSG00000149269 E008 145.2605609 0.0023672895 7.491371e-02 1.513929e-01 11 77323211 77323268 58 - 2.093 2.166 0.244
ENSG00000149269 E009 165.1664949 0.0003176953 1.202837e-01 2.203859e-01 11 77323269 77323360 92 - 2.165 2.215 0.166
ENSG00000149269 E010 12.0528060 0.0014907432 5.310713e-01 6.635702e-01 11 77325328 77325376 49 - 1.132 1.075 -0.206
ENSG00000149269 E011 2.0941023 0.0285841439 2.659748e-01 4.040975e-01 11 77329150 77329312 163 - 0.594 0.401 -0.947
ENSG00000149269 E012 182.1409974 0.0005064127 7.729741e-01 8.534756e-01 11 77332730 77332857 128 - 2.232 2.244 0.041
ENSG00000149269 E013 93.8508415 0.0003346290 2.050195e-01 3.324260e-01 11 77332858 77332867 10 - 1.980 1.936 -0.148
ENSG00000149269 E014 117.0438523 0.0003599399 8.909473e-01 9.343643e-01 11 77336086 77336115 30 - 2.042 2.051 0.028
ENSG00000149269 E015 200.0156841 0.0004136709 9.452631e-01 9.696466e-01 11 77336116 77336253 138 - 2.274 2.280 0.018
ENSG00000149269 E016 116.3607661 0.0044420922 3.031257e-01 4.450254e-01 11 77336254 77336282 29 - 2.008 2.059 0.170
ENSG00000149269 E017 170.8676453 0.0008688494 6.222067e-01 7.390361e-01 11 77337324 77337423 100 - 2.196 2.214 0.060
ENSG00000149269 E018 201.5092454 0.0023168483 7.042566e-01 8.028360e-01 11 77340646 77340763 118 - 2.271 2.286 0.049
ENSG00000149269 E019 208.1106618 0.0013603020 9.175436e-01 9.518969e-01 11 77343819 77343931 113 - 2.291 2.297 0.018
ENSG00000149269 E020 0.8041237 0.0152432768 9.078083e-01 9.454991e-01 11 77343932 77343979 48 - 0.264 0.245 -0.141
ENSG00000149269 E021 0.3634088 0.3446785713 4.712177e-01   11 77347035 77347072 38 - 0.000 0.179 10.590
ENSG00000149269 E022 113.4422085 0.0002842258 1.707612e-01 2.891585e-01 11 77349239 77349247 9 - 2.062 2.018 -0.145
ENSG00000149269 E023 131.5641398 0.0002884500 9.946475e-01 1.000000e+00 11 77349248 77349287 40 - 2.095 2.099 0.013
ENSG00000149269 E024 2.6460281 0.1010870999 1.082039e-02 3.073344e-02 11 77349288 77349546 259 - 0.810 0.303 -2.435
ENSG00000149269 E025 1.9584971 0.0071633685 5.420960e-04 2.317413e-03 11 77353131 77353535 405 - 0.748 0.178 -3.185
ENSG00000149269 E026 173.0621578 0.0002307129 1.136460e-02 3.203643e-02 11 77353536 77353599 64 - 2.260 2.194 -0.221
ENSG00000149269 E027 149.5392759 0.0002723713 2.787780e-06 2.101869e-05 11 77355668 77355704 37 - 2.241 2.107 -0.448
ENSG00000149269 E028 206.3212862 0.0002262442 3.093680e-07 2.854234e-06 11 77355705 77355842 138 - 2.372 2.246 -0.418
ENSG00000149269 E029 4.5812161 0.0036285795 3.516029e-05 2.054980e-04 11 77355843 77356298 456 - 1.009 0.443 -2.376
ENSG00000149269 E030 1.6994735 0.0083188339 2.941230e-03 1.009141e-02 11 77358841 77358897 57 - 0.678 0.178 -2.895
ENSG00000149269 E031 163.9348503 0.0002249915 3.633842e-03 1.210857e-02 11 77358898 77359017 120 - 2.243 2.164 -0.263
ENSG00000149269 E032 89.8041095 0.0003267078 2.684590e-02 6.567486e-02 11 77374328 77374365 38 - 1.984 1.903 -0.272
ENSG00000149269 E033 0.1817044 0.0397903163 7.658934e-01   11 77379195 77379240 46 - 0.000 0.098 9.659
ENSG00000149269 E034 129.0255324 0.0002727552 1.490617e-02 4.027452e-02 11 77379241 77379359 119 - 2.137 2.062 -0.249
ENSG00000149269 E035 69.0880167 0.0003472665 2.353021e-02 5.884322e-02 11 77379360 77379382 23 - 1.883 1.789 -0.316
ENSG00000149269 E036 58.5546463 0.0004364046 6.867476e-04 2.850869e-03 11 77379383 77379388 6 - 1.850 1.697 -0.517
ENSG00000149269 E037 0.1482932 0.0414734803 7.672621e-01   11 77379389 77379447 59 - 0.000 0.098 9.655
ENSG00000149269 E038 76.3696094 0.0004125577 1.947325e-03 7.063277e-03 11 77379894 77379916 23 - 1.945 1.821 -0.415
ENSG00000149269 E039 97.1062344 0.0029573244 1.317448e-03 5.035336e-03 11 77379917 77379994 78 - 2.054 1.916 -0.461
ENSG00000149269 E040 104.3130830 0.0045851293 5.216647e-04 2.240841e-03 11 77392331 77392442 112 - 2.096 1.936 -0.538
ENSG00000149269 E041 84.2375944 0.0084868288 6.346735e-04 2.661052e-03 11 77392443 77392538 96 - 2.025 1.830 -0.656
ENSG00000149269 E042 33.8162882 0.0149744378 3.735751e-02 8.614217e-02 11 77392539 77392541 3 - 1.619 1.451 -0.575
ENSG00000149269 E043 0.2987644 0.0275369308 9.586068e-02   11 77397027 77397093 67 - 0.264 0.000 -12.342
ENSG00000149269 E044 0.0000000       11 77397094 77397158 65 -      
ENSG00000149269 E045 0.0000000       11 77408154 77408232 79 -      
ENSG00000149269 E046 24.0932882 0.0010180823 6.716785e-03 2.048579e-02 11 77411774 77412064 291 - 1.493 1.306 -0.647
ENSG00000149269 E047 0.0000000       11 77455881 77455937 57 -      
ENSG00000149269 E048 0.6266857 0.0172671820 6.139143e-01 7.325143e-01 11 77473409 77473551 143 - 0.264 0.178 -0.726
ENSG00000149269 E049 45.0835350 0.0173674898 3.660381e-02 8.471976e-02 11 77473552 77474094 543 - 1.740 1.575 -0.560
ENSG00000149269 E050 2.4198596 0.0898846731 4.956254e-01 6.324037e-01 11 77474300 77474635 336 - 0.594 0.480 -0.536