ENSG00000149260

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000527129 ENSG00000149260 HEK293_OSMI2_2hA HEK293_TMG_2hB CAPN5 protein_coding retained_intron 2.552559 3.649578 2.145658 0.113642 0.2263096 -0.7635491 0.1615178 0.2254820 0.1032155 0.05430003 0.02918355 -1.056545 0.06648333 0.0613000 0.04776667 -0.01353333 0.894652926 0.007233945 FALSE TRUE
ENST00000529629 ENSG00000149260 HEK293_OSMI2_2hA HEK293_TMG_2hB CAPN5 protein_coding protein_coding 2.552559 3.649578 2.145658 0.113642 0.2263096 -0.7635491 0.1570860 0.0000000 0.1539741 0.00000000 0.15397406 4.035396 0.07417083 0.0000000 0.08716667 0.08716667 0.757563790 0.007233945 FALSE TRUE
ENST00000533889 ENSG00000149260 HEK293_OSMI2_2hA HEK293_TMG_2hB CAPN5 protein_coding retained_intron 2.552559 3.649578 2.145658 0.113642 0.2263096 -0.7635491 0.0811129 0.0000000 0.2308257 0.00000000 0.16729900 4.589918 0.04052917 0.0000000 0.09413333 0.09413333 0.133673630 0.007233945 FALSE FALSE
ENST00000648180 ENSG00000149260 HEK293_OSMI2_2hA HEK293_TMG_2hB CAPN5 protein_coding protein_coding 2.552559 3.649578 2.145658 0.113642 0.2263096 -0.7635491 1.7794175 3.1372608 1.2484843 0.20852644 0.03242848 -1.322409 0.67716250 0.8576667 0.59413333 -0.26353333 0.007233945 0.007233945 FALSE TRUE
ENST00000648752 ENSG00000149260 HEK293_OSMI2_2hA HEK293_TMG_2hB CAPN5 protein_coding protein_coding 2.552559 3.649578 2.145658 0.113642 0.2263096 -0.7635491 0.2832763 0.1557761 0.3554933 0.15577606 0.18441056 1.140609 0.10080833 0.0455000 0.15580000 0.11030000 0.551555137 0.007233945 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149260 E001 0.5546650 0.0211110650 0.89303072 0.935633482 11 77066961 77066970 10 + 0.177 0.153 -0.245
ENSG00000149260 E002 0.9996463 0.0133121044 0.30617639 0.448348567 11 77066971 77066976 6 + 0.399 0.213 -1.247
ENSG00000149260 E003 2.0982680 0.0071398434 0.07476762 0.151161109 11 77066977 77066992 16 + 0.654 0.356 -1.470
ENSG00000149260 E004 5.5928743 0.0030539649 0.08073113 0.160692923 11 77066993 77067094 102 + 0.930 0.700 -0.906
ENSG00000149260 E005 0.1451727 0.0428315769 0.20600146   11 77071610 77071682 73 + 0.176 0.000 -12.466
ENSG00000149260 E006 0.0000000       11 77072991 77073137 147 +      
ENSG00000149260 E007 10.0022424 0.0036010130 0.75237948 0.838595250 11 77084852 77084964 113 + 1.022 0.985 -0.138
ENSG00000149260 E008 10.9212623 0.0028289989 0.38143340 0.525735531 11 77084965 77085051 87 + 1.098 1.003 -0.345
ENSG00000149260 E009 0.1472490 0.0424665775 0.20626950   11 77087942 77088061 120 + 0.176 0.000 -12.468
ENSG00000149260 E010 18.4579103 0.0220807368 0.66266484 0.770930514 11 77093682 77093813 132 + 1.268 1.214 -0.189
ENSG00000149260 E011 10.1442786 0.0018617340 0.86798259 0.919091119 11 77112589 77112597 9 + 0.979 0.995 0.058
ENSG00000149260 E012 30.1944099 0.0007120156 0.41382674 0.557225147 11 77112598 77112797 200 + 1.478 1.421 -0.198
ENSG00000149260 E013 0.3030308 0.3655895848 0.10168263   11 77112798 77112911 114 + 0.303 0.000 -12.462
ENSG00000149260 E014 29.7673006 0.0074705254 0.48081260 0.619030665 11 77114242 77114434 193 + 1.471 1.415 -0.193
ENSG00000149260 E015 26.7034255 0.0013286994 0.88774259 0.932241241 11 77115395 77115588 194 + 1.390 1.377 -0.045
ENSG00000149260 E016 22.7343798 0.0008937587 0.90617979 0.944353955 11 77116226 77116303 78 + 1.313 1.319 0.022
ENSG00000149260 E017 33.6510187 0.0009208588 0.65147705 0.762352980 11 77118157 77118352 196 + 1.499 1.467 -0.110
ENSG00000149260 E018 21.4257700 0.0044817379 0.44611682 0.587647703 11 77119030 77119152 123 + 1.344 1.278 -0.231
ENSG00000149260 E019 6.2551322 0.0031844049 0.42326808 0.566359436 11 77119407 77120712 1306 + 0.876 0.768 -0.422
ENSG00000149260 E020 31.9450261 0.0012881392 0.39372422 0.537618560 11 77120713 77120909 197 + 1.506 1.448 -0.202
ENSG00000149260 E021 25.0993084 0.0008396269 0.77063539 0.851906144 11 77121934 77122049 116 + 1.381 1.357 -0.084
ENSG00000149260 E022 30.6859487 0.0227991613 0.90232873 0.941780232 11 77122576 77122712 137 + 1.455 1.444 -0.038
ENSG00000149260 E023 95.3913125 0.0006221054 0.14839735 0.259497427 11 77123688 77124374 687 + 1.885 1.937 0.177
ENSG00000149260 E024 62.1219559 0.0004182552 0.53704961 0.668698248 11 77124375 77125069 695 + 1.721 1.748 0.089
ENSG00000149260 E025 62.3073110 0.0004308319 0.00122825 0.004736655 11 77125070 77126155 1086 + 1.619 1.778 0.538