ENSG00000149089

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395787 ENSG00000149089 HEK293_OSMI2_2hA HEK293_TMG_2hB APIP protein_coding protein_coding 30.13152 22.82765 45.07006 2.423087 2.367839 0.9810755 15.080407 17.005515 20.796372 1.472623 1.0175638 0.2901746 0.5461458 0.7493333 0.4627000 -0.2866333 1.903335e-05 6.946382e-75 FALSE TRUE
ENST00000527830 ENSG00000149089 HEK293_OSMI2_2hA HEK293_TMG_2hB APIP protein_coding processed_transcript 30.13152 22.82765 45.07006 2.423087 2.367839 0.9810755 5.470751 4.942604 4.720685 1.265479 0.8311107 -0.0661381 0.1932875 0.2103333 0.1034667 -0.1068667 1.710763e-02 6.946382e-75 FALSE TRUE
MSTRG.5374.4 ENSG00000149089 HEK293_OSMI2_2hA HEK293_TMG_2hB APIP protein_coding   30.13152 22.82765 45.07006 2.423087 2.367839 0.9810755 8.627241 0.000000 19.362151 0.000000 1.2768922 10.9197684 0.2156458 0.0000000 0.4295667 0.4295667 6.946382e-75 6.946382e-75 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000149089 E001 3.7198732 0.0387119079 1.077696e-01 2.022409e-01 11 34853094 34856642 3549 - 0.772 0.484 -1.260
ENSG00000149089 E002 0.0000000       11 34859025 34859077 53 -      
ENSG00000149089 E003 0.0000000       11 34860921 34861036 116 -      
ENSG00000149089 E004 2.2465586 0.0067986151 5.575420e-03 1.748897e-02 11 34874412 34874449 38 - 0.249 0.708 2.407
ENSG00000149089 E005 3.7142674 0.2630928632 9.402283e-01 9.664635e-01 11 34875167 34875283 117 - 0.662 0.653 -0.041
ENSG00000149089 E006 0.9222279 0.0132244404 5.828406e-01 7.070919e-01 11 34882295 34882369 75 - 0.249 0.348 0.668
ENSG00000149089 E007 151.0377002 0.0031062196 1.149280e-13 3.237586e-12 11 34882370 34882675 306 - 2.040 2.322 0.943
ENSG00000149089 E008 260.7008780 0.0004050988 2.551614e-10 4.194323e-09 11 34882676 34882816 141 - 2.353 2.493 0.469
ENSG00000149089 E009 344.7745857 0.0001673619 1.814405e-03 6.645149e-03 11 34883337 34883504 168 - 2.513 2.571 0.192
ENSG00000149089 E010 305.6812369 0.0001872364 1.686855e-01 2.864605e-01 11 34888293 34888428 136 - 2.497 2.472 -0.084
ENSG00000149089 E011 272.4190969 0.0002257638 4.430874e-07 3.960816e-06 11 34888752 34888869 118 - 2.479 2.373 -0.353
ENSG00000149089 E012 184.0351884 0.0002279469 1.333670e-05 8.607042e-05 11 34890504 34890552 49 - 2.310 2.197 -0.377
ENSG00000149089 E013 3.4063324 0.0055794688 3.451047e-01 4.891825e-01 11 34890553 34890741 189 - 0.688 0.540 -0.651
ENSG00000149089 E014 215.8706528 0.0016726974 1.950535e-04 9.427270e-04 11 34895010 34895110 101 - 2.378 2.264 -0.380
ENSG00000149089 E015 6.1518759 0.0173023783 9.097735e-02 1.766790e-01 11 34896761 34896855 95 - 0.941 0.707 -0.917
ENSG00000149089 E016 163.2556559 0.0057512400 2.199098e-02 5.565742e-02 11 34916228 34916425 198 - 2.255 2.146 -0.365