ENSG00000148985

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000459679 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding protein_coding 35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 0.8926762 0.000000 1.6208222 0.0000000 0.3013875 7.3494557 0.02969583 0.00000000 0.06630000 0.066300000 4.961416e-22 4.961416e-22 FALSE  
ENST00000463452 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding protein_coding 35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 6.1976255 7.610760 4.3370973 0.8359460 0.1405788 -0.8098826 0.18034583 0.15020000 0.18073333 0.030533333 4.885211e-01 4.961416e-22 FALSE  
ENST00000464441 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding protein_coding 35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 2.0792798 3.637114 0.6669539 1.6024660 0.6669539 -2.4296258 0.05331250 0.07300000 0.02703333 -0.045966667 2.790919e-01 4.961416e-22 FALSE  
ENST00000477358 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding nonsense_mediated_decay 35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 2.0683955 3.899619 1.4939620 0.4457579 0.3130936 -1.3782599 0.05893333 0.07770000 0.06340000 -0.014300000 7.404792e-01 4.961416e-22 TRUE  
ENST00000528526 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding retained_intron 35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 2.1149111 2.698757 0.3378394 0.6888725 0.3378394 -2.9611378 0.05763750 0.05260000 0.01543333 -0.037166667 1.658170e-01 4.961416e-22    
MSTRG.5018.29 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding   35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 2.7550122 3.535387 1.7612801 0.7821156 0.1303285 -1.0011507 0.08260833 0.07006667 0.07390000 0.003833333 9.332520e-01 4.961416e-22 FALSE  
MSTRG.5018.30 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding   35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 3.0933281 6.809062 2.4089941 0.8708199 0.4613227 -1.4951661 0.08192500 0.13363333 0.09846667 -0.035166667 4.232989e-01 4.961416e-22 FALSE  
MSTRG.5018.32 ENSG00000148985 HEK293_OSMI2_2hA HEK293_TMG_2hB PGAP2 protein_coding   35.35982 50.57995 24.11955 3.00937 1.152847 -1.06805 3.2948419 6.687411 3.0090670 0.2003632 0.2301508 -1.1495007 0.08797500 0.13276667 0.12443333 -0.008333333 8.182443e-01 4.961416e-22    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148985 E001 0.2924217 0.0290785164 0.0623244255   11 3797724 3797743 20 + 0.283 0.000 -11.858
ENSG00000148985 E002 1.1092177 0.0113767926 0.1685006835 0.2862184971 11 3797819 3797897 79 + 0.453 0.228 -1.409
ENSG00000148985 E003 0.4481018 0.1913598748 0.9193067165 0.9530057759 11 3797898 3797901 4 + 0.164 0.162 -0.024
ENSG00000148985 E004 0.4481018 0.1913598748 0.9193067165 0.9530057759 11 3797902 3797907 6 + 0.164 0.162 -0.024
ENSG00000148985 E005 0.5963950 0.4439289179 1.0000000000 1.0000000000 11 3797908 3797911 4 + 0.165 0.219 0.510
ENSG00000148985 E006 1.1865344 0.0257960136 0.8696156410 0.9201586193 11 3797912 3797912 1 + 0.283 0.333 0.328
ENSG00000148985 E007 5.3476952 0.0031445992 0.1540523081 0.2671804130 11 3797913 3797922 10 + 0.871 0.712 -0.630
ENSG00000148985 E008 7.4772850 0.0022405521 0.0371771111 0.0858076532 11 3797923 3797923 1 + 1.027 0.815 -0.800
ENSG00000148985 E009 7.9953165 0.0020881650 0.0808118790 0.1608114120 11 3797924 3797924 1 + 1.027 0.859 -0.630
ENSG00000148985 E010 11.1697189 0.0038316126 0.0077296853 0.0231001654 11 3797925 3797925 1 + 1.196 0.958 -0.862
ENSG00000148985 E011 28.4913823 0.0011801121 0.0988768792 0.1887588498 11 3797926 3797938 13 + 1.476 1.398 -0.268
ENSG00000148985 E012 29.1557179 0.0014810073 0.1402363724 0.2483395032 11 3797939 3797940 2 + 1.476 1.410 -0.226
ENSG00000148985 E013 33.0192299 0.0048375170 0.2907093441 0.4315182552 11 3797941 3797946 6 + 1.508 1.471 -0.127
ENSG00000148985 E014 35.4569456 0.0012836327 0.1970156495 0.3224844153 11 3797947 3797958 12 + 1.544 1.498 -0.155
ENSG00000148985 E015 41.8676983 0.0006040512 0.1514357642 0.2636267597 11 3797959 3797971 13 + 1.617 1.571 -0.156
ENSG00000148985 E016 43.2210867 0.0005378422 0.2783524469 0.4180284590 11 3797972 3797982 11 + 1.617 1.592 -0.085
ENSG00000148985 E017 0.0000000       11 3797983 3797988 6 +      
ENSG00000148985 E018 0.0000000       11 3797989 3798100 112 +      
ENSG00000148985 E019 0.0000000       11 3808010 3808041 32 +      
ENSG00000148985 E020 0.0000000       11 3808042 3808055 14 +      
ENSG00000148985 E021 0.0000000       11 3808056 3808087 32 +      
ENSG00000148985 E022 0.7268175 0.8352044442 0.5643729900 0.6916682506 11 3808175 3808289 115 + 0.000 0.287 12.207
ENSG00000148985 E023 0.9631277 0.2964975166 0.1697744116 0.2879122472 11 3808290 3808293 4 + 0.454 0.166 -1.989
ENSG00000148985 E024 47.3843107 0.0005184743 0.1213207102 0.2218068209 11 3808294 3808315 22 + 1.671 1.624 -0.160
ENSG00000148985 E025 6.3950194 0.0026827318 0.6477817484 0.7594078520 11 3808316 3808540 225 + 0.843 0.816 -0.107
ENSG00000148985 E026 2.5883879 0.0112489207 0.0896191540 0.1745441073 11 3808541 3808553 13 + 0.670 0.418 -1.182
ENSG00000148985 E027 5.3466485 0.0032167108 0.6455246653 0.7576103882 11 3808554 3808558 5 + 0.782 0.750 -0.129
ENSG00000148985 E028 5.5680937 0.0029812793 0.7366717441 0.8270972348 11 3808559 3808559 1 + 0.782 0.767 -0.059
ENSG00000148985 E029 9.3488026 0.0018986423 0.9908662652 0.9984410772 11 3808560 3808564 5 + 0.945 0.980 0.130
ENSG00000148985 E030 20.3508774 0.0015068965 0.9892396441 0.9973114821 11 3808565 3808575 11 + 1.255 1.290 0.122
ENSG00000148985 E031 21.8149962 0.0012217774 0.8852928526 0.9306467062 11 3808576 3808578 3 + 1.277 1.324 0.166
ENSG00000148985 E032 44.9987737 0.0027785605 0.7873727706 0.8638256271 11 3808579 3808593 15 + 1.602 1.623 0.071
ENSG00000148985 E033 48.6803063 0.0010225786 0.9648401017 0.9818444977 11 3808594 3808598 5 + 1.626 1.661 0.117
ENSG00000148985 E034 83.6773551 0.0037522797 0.9622883622 0.9802061322 11 3808599 3808651 53 + 1.855 1.893 0.127
ENSG00000148985 E035 63.4402222 0.0099284141 0.4908183171 0.6281518207 11 3811250 3811266 17 + 1.766 1.765 -0.005
ENSG00000148985 E036 126.1948627 0.0005023703 0.0019345600 0.0070232949 11 3811267 3811424 158 + 2.105 2.037 -0.226
ENSG00000148985 E037 0.4460135 0.0268608969 0.0172713295 0.0455480741 11 3816189 3816254 66 + 0.376 0.000 -14.792
ENSG00000148985 E038 7.8073421 0.0058740182 0.1497978189 0.2614059008 11 3817353 3817431 79 + 0.988 0.846 -0.535
ENSG00000148985 E039 9.6884433 0.0018288332 0.0991188276 0.1891337342 11 3817432 3817535 104 + 1.080 0.935 -0.532
ENSG00000148985 E040 23.9077752 0.0011505745 0.6061632993 0.7263852707 11 3822909 3822993 85 + 1.297 1.373 0.265
ENSG00000148985 E041 4.3887295 0.0037538615 0.0785644119 0.1571893939 11 3823613 3823616 4 + 0.453 0.767 1.400
ENSG00000148985 E042 3.6857714 0.0044168569 0.0705639414 0.1442785278 11 3823617 3823619 3 + 0.376 0.712 1.592
ENSG00000148985 E043 9.7578617 0.0071784915 0.0114905654 0.0323287019 11 3823620 3823740 121 + 0.710 1.073 1.392
ENSG00000148985 E044 6.4159923 0.0694629671 0.0579740317 0.1231568686 11 3823741 3823882 142 + 0.518 0.914 1.652
ENSG00000148985 E045 252.0316458 0.0002071688 0.0001588931 0.0007868932 11 3823883 3824135 253 + 2.394 2.343 -0.168
ENSG00000148985 E046 9.8060544 0.0050958443 0.9119978830 0.9481809664 11 3824136 3824269 134 + 0.988 1.009 0.079
ENSG00000148985 E047 229.7191912 0.0012044187 0.0293277879 0.0706392737 11 3824270 3824364 95 + 2.338 2.314 -0.079
ENSG00000148985 E048 102.0795505 0.0139384523 0.3406960708 0.4846403672 11 3824365 3824376 12 + 1.994 1.965 -0.097
ENSG00000148985 E049 31.8777500 0.0054809018 0.2903356247 0.4311339971 11 3824377 3824449 73 + 1.508 1.467 -0.139
ENSG00000148985 E050 15.4315047 0.0011427989 0.7533946906 0.8393552201 11 3824450 3824455 6 + 1.127 1.191 0.230
ENSG00000148985 E051 11.6172078 0.0017740562 0.6347082404 0.7491152616 11 3824456 3824570 115 + 1.063 1.048 -0.055
ENSG00000148985 E052 13.4514124 0.0026241484 0.0821915389 0.1629645882 11 3824571 3824735 165 + 0.945 1.165 0.805
ENSG00000148985 E053 12.3591637 0.0023795282 0.3885199704 0.5326042027 11 3824736 3824795 60 + 0.988 1.114 0.461
ENSG00000148985 E054 26.7974614 0.0014141781 0.0498180996 0.1088373423 11 3824796 3825019 224 + 1.255 1.444 0.657
ENSG00000148985 E055 239.3709010 0.0002716767 0.8315283784 0.8944095016 11 3825020 3825128 109 + 2.311 2.344 0.109
ENSG00000148985 E056 1006.8827814 0.0065421681 0.0009579198 0.0038146123 11 3825328 3826371 1044 + 2.862 2.998 0.452