ENSG00000148835

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369839 ENSG00000148835 HEK293_OSMI2_2hA HEK293_TMG_2hB TAF5 protein_coding protein_coding 9.491592 8.488487 11.8168 0.8155605 0.5406479 0.4767816 6.4529357 3.8907319 9.331370 0.3508909 0.5192617 1.259889 0.64619583 0.46183333 0.78903333 0.32720000 8.685283e-09 3.048325e-30 FALSE TRUE
ENST00000692195 ENSG00000148835 HEK293_OSMI2_2hA HEK293_TMG_2hB TAF5 protein_coding protein_coding 9.491592 8.488487 11.8168 0.8155605 0.5406479 0.4767816 0.4941809 0.2597055 1.011106 0.2020822 0.1764900 1.920676 0.04560417 0.03386667 0.08723333 0.05336667 2.575907e-01 3.048325e-30 FALSE TRUE
ENST00000693184 ENSG00000148835 HEK293_OSMI2_2hA HEK293_TMG_2hB TAF5 protein_coding retained_intron 9.491592 8.488487 11.8168 0.8155605 0.5406479 0.4767816 1.5952371 3.9047669 0.000000 0.8012275 0.0000000 -8.612783 0.20463333 0.45100000 0.00000000 -0.45100000 3.048325e-30 3.048325e-30   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148835 E001 2.4682114 0.0076381383 1.043396e-01 1.970960e-01 10 103367966 103367971 6 + 0.623 0.360 -1.310
ENSG00000148835 E002 3.1315174 0.0346646454 1.272790e-01 2.303460e-01 10 103367972 103367975 4 + 0.693 0.436 -1.190
ENSG00000148835 E003 4.6899828 0.0236734159 2.493624e-01 3.851688e-01 10 103367976 103367980 5 + 0.807 0.647 -0.655
ENSG00000148835 E004 7.2030446 0.0694970885 2.770000e-01 4.165025e-01 10 103367981 103367989 9 + 0.972 0.813 -0.604
ENSG00000148835 E005 59.4433361 0.0006098739 1.084144e-06 8.931844e-06 10 103367990 103368548 559 + 1.849 1.639 -0.710
ENSG00000148835 E006 0.1515154 0.0430009492 7.835094e-01   10 103368549 103368567 19 + 0.096 0.000 -9.371
ENSG00000148835 E007 80.8122494 0.0016235875 1.076653e-05 7.101233e-05 10 103373358 103373595 238 + 1.965 1.796 -0.569
ENSG00000148835 E008 5.2810501 0.0358375019 5.460228e-01 6.762708e-01 10 103373596 103375103 1508 + 0.735 0.848 0.450
ENSG00000148835 E009 124.6602150 0.0003198156 2.138812e-06 1.652898e-05 10 103378235 103378550 316 + 2.140 2.014 -0.422
ENSG00000148835 E010 57.2873821 0.0034260288 1.809952e-01 3.024654e-01 10 103379608 103379771 164 + 1.779 1.731 -0.160
ENSG00000148835 E011 54.9310924 0.0004735996 9.200724e-02 1.782423e-01 10 103379884 103380019 136 + 1.768 1.714 -0.184
ENSG00000148835 E012 67.7277762 0.0004081105 5.037459e-02 1.098327e-01 10 103381721 103381841 121 + 1.852 1.793 -0.200
ENSG00000148835 E013 7.8312139 0.0821905128 3.656681e-01 5.099842e-01 10 103381842 103383237 1396 + 0.984 0.850 -0.506
ENSG00000148835 E014 76.5451392 0.0004527677 2.877315e-01 4.282477e-01 10 103383238 103383367 130 + 1.890 1.872 -0.058
ENSG00000148835 E015 44.4770874 0.0005299513 8.572917e-01 9.118700e-01 10 103385326 103385369 44 + 1.644 1.660 0.054
ENSG00000148835 E016 74.3320948 0.0003807121 5.637685e-01 6.912002e-01 10 103385370 103385490 121 + 1.852 1.901 0.165
ENSG00000148835 E017 0.2924217 0.0290785164 3.794987e-01   10 103385491 103385618 128 + 0.174 0.000 -10.371
ENSG00000148835 E018 0.5858876 0.0192825225 5.969929e-01 7.188343e-01 10 103386941 103387115 175 + 0.240 0.155 -0.781
ENSG00000148835 E019 0.7310603 0.0526193777 4.135461e-01 5.570136e-01 10 103387116 103387174 59 + 0.298 0.155 -1.198
ENSG00000148835 E020 103.0773589 0.0005163267 9.290939e-02 1.796819e-01 10 103387175 103387352 178 + 1.977 2.060 0.280
ENSG00000148835 E021 2.5840118 0.0093372462 7.503022e-02 1.515783e-01 10 103387353 103387520 168 + 0.648 0.360 -1.418
ENSG00000148835 E022 127.8271799 0.0038958561 1.386013e-02 3.790944e-02 10 103387521 103387698 178 + 2.051 2.175 0.416
ENSG00000148835 E023 1.9531840 0.0075895583 7.530788e-01 8.391230e-01 10 103387699 103388005 307 + 0.473 0.435 -0.194
ENSG00000148835 E024 287.7070780 0.0032122776 3.433643e-09 4.609832e-08 10 103388006 103389065 1060 + 2.367 2.559 0.642