ENSG00000148484

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000345264 ENSG00000148484 HEK293_OSMI2_2hA HEK293_TMG_2hB RSU1 protein_coding protein_coding 52.10475 68.58849 48.70559 2.344468 0.966595 -0.4937932 3.48073 2.023903 6.229155 0.3429213 0.2488371 1.6170999 0.0712250 0.0298000 0.1279000 0.0981 4.888834e-09 1.614167e-13 FALSE TRUE
ENST00000377911 ENSG00000148484 HEK293_OSMI2_2hA HEK293_TMG_2hB RSU1 protein_coding processed_transcript 52.10475 68.58849 48.70559 2.344468 0.966595 -0.4937932 28.90528 36.864961 27.801070 2.3026269 1.5091697 -0.4069823 0.5560042 0.5369333 0.5702333 0.0333 7.324216e-01 1.614167e-13 FALSE TRUE
MSTRG.3609.4 ENSG00000148484 HEK293_OSMI2_2hA HEK293_TMG_2hB RSU1 protein_coding   52.10475 68.58849 48.70559 2.344468 0.966595 -0.4937932 16.95341 27.179558 13.810048 1.5028148 0.3911404 -0.9762901 0.3176333 0.3963333 0.2835333 -0.1128 4.438529e-04 1.614167e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148484 E001 0.1482932 0.0413594395 7.041766e-01   10 16590611 16590619 9 - 0.000 0.102 9.631
ENSG00000148484 E002 166.1038914 0.0067142736 5.799406e-39 1.878086e-36 10 16590620 16592552 1933 - 2.503 1.867 -2.127
ENSG00000148484 E003 205.2498356 0.0084661966 1.005566e-02 2.889757e-02 10 16592553 16593040 488 - 2.206 2.339 0.446
ENSG00000148484 E004 319.3603401 0.0015438534 3.211133e-15 1.136446e-13 10 16593041 16593152 112 - 2.333 2.554 0.737
ENSG00000148484 E005 492.5853037 0.0035972916 3.350367e-05 1.968725e-04 10 16593153 16593312 160 - 2.586 2.716 0.433
ENSG00000148484 E006 545.4938199 0.0003675852 3.517820e-06 2.593557e-05 10 16593313 16593496 184 - 2.665 2.745 0.265
ENSG00000148484 E007 417.7887604 0.0002456857 3.747921e-01 5.192345e-01 10 16695023 16695155 133 - 2.591 2.608 0.054
ENSG00000148484 E008 0.3299976 0.0274424043 3.381025e-01   10 16727031 16727157 127 - 0.000 0.184 10.635
ENSG00000148484 E009 429.4836390 0.0001572165 6.236274e-01 7.401600e-01 10 16752539 16752653 115 - 2.609 2.618 0.029
ENSG00000148484 E010 380.7908481 0.0001827202 1.603541e-01 2.756114e-01 10 16752918 16753000 83 - 2.580 2.554 -0.087
ENSG00000148484 E011 479.0737140 0.0001302948 1.004491e-01 1.911504e-01 10 16754871 16754989 119 - 2.679 2.652 -0.089
ENSG00000148484 E012 496.8406549 0.0008487913 1.675170e-01 2.849638e-01 10 16764390 16764510 121 - 2.694 2.666 -0.094
ENSG00000148484 E013 330.1904978 0.0017203246 3.859249e-02 8.843876e-02 10 16782034 16782084 51 - 2.534 2.478 -0.189
ENSG00000148484 E014 291.8399283 0.0005748043 1.660364e-03 6.154753e-03 10 16816973 16817084 112 - 2.492 2.419 -0.243
ENSG00000148484 E015 5.6086036 0.0033995543 2.214901e-01 3.521960e-01 10 16817085 16817314 230 - 0.700 0.865 0.659
ENSG00000148484 E016 257.5323320 0.0025798987 2.484534e-02 6.155694e-02 10 16817315 16817478 164 - 2.437 2.363 -0.244