ENSG00000148411

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000277554 ENSG00000148411 HEK293_OSMI2_2hA HEK293_TMG_2hB NACC2 protein_coding protein_coding 5.331408 7.170853 5.010222 0.7856631 0.1305769 -0.5164039 2.3202069 0.2397701 4.767897 0.2397701 0.0513352 4.257703 0.41583333 0.02886667 0.9525667 0.9237 1.498714e-05 7.593919e-55 FALSE TRUE
ENST00000371753 ENSG00000148411 HEK293_OSMI2_2hA HEK293_TMG_2hB NACC2 protein_coding protein_coding 5.331408 7.170853 5.010222 0.7856631 0.1305769 -0.5164039 2.5022303 5.8830711 0.000000 0.5845489 0.0000000 -9.202876 0.49894167 0.82280000 0.0000000 -0.8228 7.593919e-55 7.593919e-55 FALSE TRUE
MSTRG.33617.3 ENSG00000148411 HEK293_OSMI2_2hA HEK293_TMG_2hB NACC2 protein_coding   5.331408 7.170853 5.010222 0.7856631 0.1305769 -0.5164039 0.3140224 1.0480115 0.000000 0.2446407 0.0000000 -6.725212 0.05621667 0.14830000 0.0000000 -0.1483 2.755388e-09 7.593919e-55 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148411 E001 806.729329 0.0024050445 7.364814e-01 8.269619e-01 9 136006537 136011707 5171 - 2.879 2.892 0.042
ENSG00000148411 E002 38.218263 0.0034276109 6.475581e-01 7.592021e-01 9 136011708 136011803 96 - 1.583 1.556 -0.093
ENSG00000148411 E003 28.338381 0.0008824091 1.444226e-01 2.540454e-01 9 136011804 136011827 24 - 1.376 1.475 0.342
ENSG00000148411 E004 66.529852 0.0004913530 4.257822e-01 5.686832e-01 9 136011828 136012024 197 - 1.783 1.818 0.117
ENSG00000148411 E005 55.180847 0.0006250947 2.242725e-01 3.555341e-01 9 136013199 136013296 98 - 1.690 1.748 0.200
ENSG00000148411 E006 62.151011 0.0040102639 3.293490e-01 4.728551e-01 9 136013864 136013969 106 - 1.748 1.800 0.176
ENSG00000148411 E007 36.687899 0.0026676666 7.711465e-01 8.522573e-01 9 136016265 136016290 26 - 1.546 1.564 0.060
ENSG00000148411 E008 66.792323 0.0006330677 2.328084e-01 3.657695e-01 9 136016291 136016429 139 - 1.778 1.830 0.177
ENSG00000148411 E009 0.000000       9 136019168 136019480 313 -      
ENSG00000148411 E010 60.400450 0.0004604515 8.347353e-01 8.965456e-01 9 136049636 136049792 157 - 1.769 1.759 -0.034
ENSG00000148411 E011 22.013056 0.0008754795 8.633360e-01 9.158412e-01 9 136049793 136049810 18 - 1.331 1.343 0.043
ENSG00000148411 E012 21.611898 0.0012255651 5.958579e-01 7.178783e-01 9 136049811 136049838 28 - 1.362 1.321 -0.141
ENSG00000148411 E013 46.465341 0.0005268940 2.804758e-03 9.688797e-03 9 136049839 136050155 317 - 1.745 1.591 -0.521
ENSG00000148411 E014 61.958996 0.0016996178 2.346915e-01 3.679987e-01 9 136050156 136050580 425 - 1.810 1.754 -0.191
ENSG00000148411 E015 9.022092 0.0026314027 1.001435e-07 1.016659e-06 9 136095189 136095289 101 - 1.262 0.679 -2.195