ENSG00000148400

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000491649 ENSG00000148400 HEK293_OSMI2_2hA HEK293_TMG_2hB NOTCH1 protein_coding retained_intron 6.689768 6.679852 6.486089 0.9147439 0.1820079 -0.04240295 0.2187252 0.3571765 0.000000 0.1114368 0.0000000 -5.198402 0.03125417 0.05036667 0.0000000 -0.05036667 5.509968e-06 3.982463e-30 FALSE FALSE
ENST00000651671 ENSG00000148400 HEK293_OSMI2_2hA HEK293_TMG_2hB NOTCH1 protein_coding protein_coding 6.689768 6.679852 6.486089 0.9147439 0.1820079 -0.04240295 4.4654008 2.6340525 5.577489 0.5721584 0.1711404 1.079449 0.66923750 0.38666667 0.8597667 0.47310000 1.646015e-15 3.982463e-30 FALSE TRUE
ENST00000680778 ENSG00000148400 HEK293_OSMI2_2hA HEK293_TMG_2hB NOTCH1 protein_coding protein_coding 6.689768 6.679852 6.486089 0.9147439 0.1820079 -0.04240295 1.2847506 3.0132554 0.000000 0.2127412 0.0000000 -8.239959 0.19074167 0.46100000 0.0000000 -0.46100000 3.982463e-30 3.982463e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148400 E001 0.0000000       9 136494433 136494443 11 -      
ENSG00000148400 E002 4.3310204 0.0158690968 1.103557e-02 3.124720e-02 9 136494444 136494456 13 - 0.480 0.901 1.784
ENSG00000148400 E003 280.7320488 0.0171898978 3.969574e-11 7.488785e-10 9 136494457 136495462 1006 - 2.160 2.629 1.565
ENSG00000148400 E004 72.0658651 0.0075998346 1.346801e-06 1.087817e-05 9 136495463 136495688 226 - 1.663 2.000 1.136
ENSG00000148400 E005 32.9664995 0.0189950393 4.789547e-05 2.711062e-04 9 136495689 136495735 47 - 1.270 1.693 1.454
ENSG00000148400 E006 130.8538371 0.0065395850 1.479225e-07 1.453801e-06 9 136495736 136496229 494 - 1.930 2.248 1.066
ENSG00000148400 E007 262.4570334 0.0002493076 5.359997e-05 2.997427e-04 9 136496230 136497558 1329 - 2.339 2.478 0.465
ENSG00000148400 E008 47.1604187 0.0006866752 7.828423e-01 8.605646e-01 9 136498899 136498996 98 - 1.646 1.710 0.216
ENSG00000148400 E009 0.3268771 0.0293979284 9.299564e-01   9 136498997 136499111 115 - 0.110 0.136 0.347
ENSG00000148400 E010 61.7840054 0.0004105574 3.540818e-01 4.983494e-01 9 136499112 136499259 148 - 1.745 1.836 0.308
ENSG00000148400 E011 4.8736352 0.0032769252 1.376423e-01 2.448006e-01 9 136499260 136500551 1292 - 0.831 0.669 -0.653
ENSG00000148400 E012 89.3657801 0.0067272926 9.234819e-01 9.557807e-01 9 136500552 136500847 296 - 1.916 1.979 0.211
ENSG00000148400 E013 59.6822191 0.0162190230 8.410579e-01 9.008441e-01 9 136501748 136501913 166 - 1.761 1.795 0.114
ENSG00000148400 E014 39.8462555 0.0016330248 3.645639e-01 5.089201e-01 9 136502001 136502088 88 - 1.552 1.654 0.348
ENSG00000148400 E015 43.6178165 0.0119052633 4.474451e-01 5.888283e-01 9 136502272 136502436 165 - 1.586 1.702 0.394
ENSG00000148400 E016 26.1584204 0.0045881144 3.950325e-01 5.388699e-01 9 136502437 136502488 52 - 1.377 1.487 0.382
ENSG00000148400 E017 3.9106500 0.0040771493 5.638314e-03 1.765323e-02 9 136502489 136502775 287 - 0.831 0.453 -1.653
ENSG00000148400 E018 3.2902027 0.0046846206 5.403933e-01 6.715463e-01 9 136502776 136503181 406 - 0.650 0.595 -0.238
ENSG00000148400 E019 42.6901801 0.0005168371 3.273364e-01 4.707755e-01 9 136503182 136503330 149 - 1.582 1.684 0.347
ENSG00000148400 E020 82.5407766 0.0004010878 4.636134e-01 6.033908e-01 9 136504673 136505104 432 - 1.873 1.952 0.266
ENSG00000148400 E021 93.8995289 0.0055038971 1.766551e-01 2.968992e-01 9 136505310 136505881 572 - 1.975 1.967 -0.030
ENSG00000148400 E022 32.6296324 0.0006302065 8.413977e-01 9.010858e-01 9 136506527 136506639 113 - 1.500 1.537 0.127
ENSG00000148400 E023 36.0095628 0.0007927275 4.331467e-02 9.714035e-02 9 136506716 136506835 120 - 1.471 1.639 0.576
ENSG00000148400 E024 49.2627120 0.0041266385 8.615178e-01 9.146807e-01 9 136506836 136506973 138 - 1.671 1.716 0.151
ENSG00000148400 E025 51.2113856 0.0117558932 2.849821e-01 4.252776e-01 9 136507305 136507437 133 - 1.721 1.706 -0.054
ENSG00000148400 E026 51.3621928 0.0012840027 1.684256e-01 2.861327e-01 9 136507955 136508139 185 - 1.724 1.704 -0.067
ENSG00000148400 E027 29.8732136 0.0029490975 8.340041e-01 8.960519e-01 9 136508232 136508292 61 - 1.449 1.514 0.223
ENSG00000148400 E028 31.2249899 0.0136496150 2.429580e-01 3.776609e-01 9 136508293 136508385 93 - 1.523 1.481 -0.142
ENSG00000148400 E029 46.1287469 0.0006800473 3.577864e-01 5.022035e-01 9 136508870 136509071 202 - 1.666 1.668 0.007
ENSG00000148400 E030 0.0000000       9 136509072 136509343 272 -      
ENSG00000148400 E031 53.9432609 0.0004611718 1.235586e-03 4.761363e-03 9 136509733 136509961 229 - 1.784 1.678 -0.358
ENSG00000148400 E032 0.0000000       9 136510510 136510652 143 -      
ENSG00000148400 E033 49.5084242 0.0004637677 1.385321e-06 1.115600e-05 9 136510653 136510800 148 - 1.784 1.589 -0.661
ENSG00000148400 E034 22.5825873 0.0034530074 3.226066e-05 1.903892e-04 9 136510801 136510805 5 - 1.484 1.206 -0.968
ENSG00000148400 E035 46.0860256 0.0004835122 2.369782e-04 1.120605e-03 9 136511152 136511271 120 - 1.731 1.589 -0.483
ENSG00000148400 E036 0.0000000       9 136512693 136512773 81 -      
ENSG00000148400 E037 0.0000000       9 136512774 136512848 75 -      
ENSG00000148400 E038 48.4566682 0.0014861753 2.879618e-03 9.909990e-03 9 136513021 136513134 114 - 1.740 1.632 -0.367
ENSG00000148400 E039 57.6291824 0.0022925969 3.040347e-02 7.275198e-02 9 136513392 136513537 146 - 1.792 1.732 -0.202
ENSG00000148400 E040 0.1451727 0.0440651103 5.456013e-01   9 136513538 136513551 14 - 0.110 0.000 -9.692
ENSG00000148400 E041 58.9377425 0.0013028546 2.817814e-04 1.305646e-03 9 136514510 136514702 193 - 1.827 1.701 -0.424
ENSG00000148400 E042 48.0619899 0.0004571784 4.548242e-10 7.176834e-09 9 136515290 136515400 111 - 1.798 1.522 -0.935
ENSG00000148400 E043 65.4825863 0.0003831673 3.915543e-09 5.201878e-08 9 136515483 136515716 234 - 1.908 1.697 -0.712
ENSG00000148400 E044 39.1396974 0.0005632338 2.553501e-05 1.544747e-04 9 136515981 136516094 114 - 1.682 1.493 -0.645
ENSG00000148400 E045 33.3217253 0.0109408724 1.365096e-03 5.193039e-03 9 136517272 136517385 114 - 1.620 1.422 -0.679
ENSG00000148400 E046 38.5272821 0.0015269167 9.627618e-05 5.045990e-04 9 136517752 136517937 186 - 1.668 1.488 -0.615
ENSG00000148400 E047 39.5255526 0.0007474126 2.326913e-08 2.678333e-07 9 136518137 136518292 156 - 1.712 1.438 -0.934
ENSG00000148400 E048 57.5516193 0.0004387375 1.052711e-10 1.850370e-09 9 136518591 136518824 234 - 1.868 1.609 -0.875
ENSG00000148400 E049 43.6443419 0.0005269902 4.630609e-09 6.076857e-08 9 136519443 136519565 123 - 1.754 1.482 -0.924
ENSG00000148400 E050 2.8574474 0.0189618782 7.590300e-06 5.181649e-05 9 136522572 136522849 278 - 0.000 0.815 13.348
ENSG00000148400 E051 54.5175676 0.0004063532 1.821869e-07 1.759431e-06 9 136522850 136523188 339 - 1.827 1.624 -0.685
ENSG00000148400 E052 33.4718378 0.0067972089 6.667492e-03 2.035773e-02 9 136523717 136523979 263 - 1.592 1.453 -0.478
ENSG00000148400 E053 13.9312541 0.0061950090 4.483561e-04 1.960706e-03 9 136544024 136544102 79 - 1.289 0.992 -1.066
ENSG00000148400 E054 7.9461732 0.0173992140 2.203598e-04 1.050967e-03 9 136545726 136546048 323 - 1.118 0.669 -1.724