ENSG00000148229

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374169 ENSG00000148229 HEK293_OSMI2_2hA HEK293_TMG_2hB POLE3 protein_coding protein_coding 119.4931 128.4798 141.3551 24.01991 3.535431 0.1377717 79.13379 87.72709 77.99841 16.982181 4.839733 -0.1695572 0.6772500 0.6841000 0.5509333 -0.1331667 0.02313268 1.523671e-12 FALSE TRUE
ENST00000374171 ENSG00000148229 HEK293_OSMI2_2hA HEK293_TMG_2hB POLE3 protein_coding protein_coding 119.4931 128.4798 141.3551 24.01991 3.535431 0.1377717 35.85149 38.54396 55.07590 7.799431 2.331109 0.5148040 0.2849458 0.2986333 0.3905333 0.0919000 0.15952461 1.523671e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148229 E001 6257.25479 0.0012988997 3.070956e-20 2.015183e-18 9 113407235 113408943 1709 - 3.724 3.860 0.453
ENSG00000148229 E002 831.06647 0.0006467772 5.545825e-11 1.021427e-09 9 113408944 113408983 40 - 2.941 2.909 -0.105
ENSG00000148229 E003 1075.14461 0.0008081018 9.106651e-13 2.251374e-11 9 113409610 113409728 119 - 3.057 3.014 -0.143
ENSG00000148229 E004 24.13379 0.0007661082 1.773823e-18 9.476746e-17 9 113409729 113410054 326 - 1.607 1.012 -2.087
ENSG00000148229 E005 785.56952 0.0011464882 1.542469e-07 1.510863e-06 9 113410055 113410140 86 - 2.915 2.884 -0.104
ENSG00000148229 E006 25.80285 0.0007242083 6.045574e-06 4.224699e-05 9 113410141 113410227 87 - 1.534 1.307 -0.785
ENSG00000148229 E007 752.41849 0.0005754870 3.315467e-13 8.752861e-12 9 113410228 113410408 181 - 2.904 2.861 -0.142
ENSG00000148229 E008 70.03837 0.0035966926 3.955287e-01 5.393388e-01 9 113410617 113410675 59 - 1.840 1.877 0.126