ENSG00000148175

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000286713 ENSG00000148175 HEK293_OSMI2_2hA HEK293_TMG_2hB STOM protein_coding protein_coding 16.65603 10.55064 22.89866 1.503782 0.3536821 1.117196 12.971003 10.3391177 15.262798 1.46240196 0.6132463 0.5614566 0.8414583 0.98023333 0.6660333 -0.3142000 5.597661e-35 5.597661e-35 FALSE TRUE
ENST00000347359 ENSG00000148175 HEK293_OSMI2_2hA HEK293_TMG_2hB STOM protein_coding protein_coding 16.65603 10.55064 22.89866 1.503782 0.3536821 1.117196 1.286506 0.0000000 2.351283 0.00000000 0.3009703 7.8834270 0.0510250 0.00000000 0.1025667 0.1025667 3.973873e-15 5.597661e-35 FALSE TRUE
MSTRG.33312.1 ENSG00000148175 HEK293_OSMI2_2hA HEK293_TMG_2hB STOM protein_coding   16.65603 10.55064 22.89866 1.503782 0.3536821 1.117196 2.398524 0.2115204 5.284583 0.04143417 0.4994120 4.5790055 0.1075167 0.01976667 0.2314333 0.2116667 3.725924e-16 5.597661e-35 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148175 E001 2.778312 0.0068905732 7.335428e-01 8.247329e-01 9 121328692 121328756 65 - 0.553 0.632 0.354
ENSG00000148175 E002 2.395785 0.1637462809 2.336407e-01 3.667244e-01 9 121329533 121329625 93 - 0.405 0.666 1.234
ENSG00000148175 E003 0.000000       9 121338987 121338987 1 -      
ENSG00000148175 E004 216.765353 0.0072249298 2.868608e-07 2.665796e-06 9 121338988 121339641 654 - 2.205 2.467 0.875
ENSG00000148175 E005 417.018152 0.0016762709 4.625128e-11 8.633527e-10 9 121339642 121340769 1128 - 2.539 2.712 0.577
ENSG00000148175 E006 318.623854 0.0017953943 2.107759e-01 3.394788e-01 9 121340770 121341408 639 - 2.506 2.500 -0.021
ENSG00000148175 E007 127.523394 0.0004691948 3.374852e-02 7.925077e-02 9 121348015 121348149 135 - 2.122 2.085 -0.124
ENSG00000148175 E008 82.807049 0.0080862008 1.339521e-02 3.683300e-02 9 121349120 121349158 39 - 1.969 1.850 -0.400
ENSG00000148175 E009 163.633735 0.0003009540 2.400128e-05 1.462330e-04 9 121349159 121349323 165 - 2.251 2.165 -0.287
ENSG00000148175 E010 113.327177 0.0003233250 3.556709e-03 1.188730e-02 9 121353220 121353302 83 - 2.084 2.018 -0.220
ENSG00000148175 E011 98.533849 0.0003431228 5.993569e-04 2.530789e-03 9 121354601 121354673 73 - 2.032 1.940 -0.311
ENSG00000148175 E012 113.082997 0.0029064496 1.850987e-07 1.785169e-06 9 121356053 121356156 104 - 2.126 1.933 -0.646
ENSG00000148175 E013 80.451290 0.0091329066 1.232393e-04 6.287085e-04 9 121370127 121370304 178 - 1.985 1.770 -0.724