ENSG00000148158

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374232 ENSG00000148158 HEK293_OSMI2_2hA HEK293_TMG_2hB SNX30 protein_coding protein_coding 4.715247 2.96386 5.978433 0.2822558 0.1521318 1.009842 1.409224 0.1970412 2.980739 0.05649097 0.5326927 3.8525122 0.2611792 0.07166667 0.5024333 0.4307667 0.0003963070 0.000396307 FALSE TRUE
MSTRG.33251.2 ENSG00000148158 HEK293_OSMI2_2hA HEK293_TMG_2hB SNX30 protein_coding   4.715247 2.96386 5.978433 0.2822558 0.1521318 1.009842 3.175927 2.7150473 2.941391 0.33351974 0.6353774 0.1151139 0.7123958 0.91096667 0.4881667 -0.4228000 0.0005861862 0.000396307 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000148158 E001 13.5984476 0.0231108169 1.125419e-06 9.244154e-06 9 112750760 112751157 398 + 1.267 0.656 -2.311
ENSG00000148158 E002 0.2944980 0.3613765914 5.668683e-01   9 112804108 112804165 58 + 0.153 0.000 -8.937
ENSG00000148158 E003 26.5307216 0.0320628235 1.540045e-03 5.766175e-03 9 112804776 112804967 192 + 1.491 1.198 -1.019
ENSG00000148158 E004 23.2673017 0.0008274289 1.384214e-07 1.369088e-06 9 112817705 112817815 111 + 1.450 1.099 -1.235
ENSG00000148158 E005 29.9575899 0.0016320222 9.390894e-08 9.586059e-07 9 112830725 112830883 159 + 1.551 1.245 -1.062
ENSG00000148158 E006 33.8427876 0.0014863493 1.307237e-05 8.449120e-05 9 112836214 112836409 196 + 1.583 1.381 -0.694
ENSG00000148158 E007 24.6100126 0.0026616577 8.202396e-03 2.429738e-02 9 112838498 112838584 87 + 1.427 1.313 -0.395
ENSG00000148158 E008 23.6492650 0.0016286741 3.522706e-04 1.589232e-03 9 112838585 112838697 113 + 1.427 1.232 -0.679
ENSG00000148158 E009 24.0673933 0.0008462756 7.255254e-05 3.924300e-04 9 112850859 112850945 87 + 1.440 1.219 -0.771
ENSG00000148158 E010 0.0000000       9 112856708 112856803 96 +      
ENSG00000148158 E011 31.1111725 0.0080584004 1.022581e-04 5.324308e-04 9 112864247 112864399 153 + 1.551 1.323 -0.787
ENSG00000148158 E012 0.1451727 0.0431802846 1.000000e+00   9 112866485 112866525 41 + 0.083 0.000 -8.661
ENSG00000148158 E013 740.5332720 0.0029032076 2.145087e-25 2.433658e-23 9 112868784 112874987 6204 + 2.780 2.956 0.587
ENSG00000148158 E014 0.6632043 0.0172671820 1.000833e-01 1.906055e-01 9 112875169 112875291 123 + 0.083 0.399 2.836
ENSG00000148158 E015 0.5921286 0.1710577615 1.678672e-01 2.854188e-01 9 112875832 112875929 98 + 0.083 0.392 2.800
ENSG00000148158 E016 5.0717474 0.0088317514 2.328015e-02 5.833185e-02 9 112877119 112880472 3354 + 0.597 0.979 1.532
ENSG00000148158 E017 0.5117906 0.2313595095 9.820061e-01 9.927539e-01 9 112881428 112881671 244 + 0.152 0.179 0.279