ENSG00000147669

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000353107 ENSG00000147669 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR2K protein_coding protein_coding 63.54162 50.06158 91.74591 5.965157 2.479791 0.8738092 60.36141 49.37276 85.82301 6.014845 2.070135 0.7975252 0.9569875 0.9856 0.9356 -0.05 7.387567e-08 7.387567e-08    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147669 E001 0.701914 0.0181680384 6.356712e-01 7.498879e-01 8 100150607 100150617 11 + 0.176 0.266 0.756
ENSG00000147669 E002 1.001723 0.0135132673 5.666472e-01 6.935894e-01 8 100150618 100150622 5 + 0.244 0.356 0.754
ENSG00000147669 E003 1.001723 0.0135132673 5.666472e-01 6.935894e-01 8 100150623 100150624 2 + 0.244 0.356 0.754
ENSG00000147669 E004 3.752779 0.0045307381 7.043884e-02 1.440747e-01 8 100150625 100150635 11 + 0.761 0.493 -1.172
ENSG00000147669 E005 388.514064 0.0003316552 5.084094e-08 5.472536e-07 8 100150636 100150709 74 + 2.619 2.536 -0.278
ENSG00000147669 E006 5.385329 0.0030935348 2.116794e-02 5.395206e-02 8 100150710 100150782 73 + 0.904 0.597 -1.247
ENSG00000147669 E007 2.962283 0.0049694404 2.727729e-01 4.117250e-01 8 100151334 100151346 13 + 0.654 0.493 -0.732
ENSG00000147669 E008 613.041975 0.0002172798 2.169013e-03 7.746937e-03 8 100151347 100151416 70 + 2.795 2.771 -0.079
ENSG00000147669 E009 5.756980 0.0108810996 8.209698e-01 8.871526e-01 8 100151417 100151823 407 + 0.846 0.838 -0.032
ENSG00000147669 E010 728.312825 0.0001368201 6.633644e-02 1.372824e-01 8 100151824 100151916 93 + 2.862 2.860 -0.007
ENSG00000147669 E011 10.211629 0.0044717242 6.595954e-04 2.750812e-03 8 100151917 100152209 293 + 1.162 0.783 -1.416
ENSG00000147669 E012 1036.914986 0.0011096983 1.440366e-09 2.072401e-08 8 100153294 100154003 710 + 2.968 3.068 0.333