ENSG00000147649

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336273 ENSG00000147649 HEK293_OSMI2_2hA HEK293_TMG_2hB MTDH protein_coding protein_coding 47.42244 7.155378 90.77521 0.6186129 2.208931 3.663343 24.804426 5.4357422 44.471353 0.2432735 1.5987899 3.030000 0.62682500 0.77536667 0.4899667 -0.28540000 5.273356e-02 8.150294e-08 FALSE TRUE
ENST00000519934 ENSG00000147649 HEK293_OSMI2_2hA HEK293_TMG_2hB MTDH protein_coding protein_coding 47.42244 7.155378 90.77521 0.6186129 2.208931 3.663343 1.497269 0.6893211 2.332781 0.1474792 0.1638938 1.744195 0.06015833 0.09443333 0.0257000 -0.06873333 8.150294e-08 8.150294e-08 FALSE TRUE
ENST00000522313 ENSG00000147649 HEK293_OSMI2_2hA HEK293_TMG_2hB MTDH protein_coding protein_coding 47.42244 7.155378 90.77521 0.6186129 2.208931 3.663343 19.689315 0.1880214 40.696772 0.1880214 1.7159432 7.683469 0.25175417 0.02240000 0.4482667 0.42586667 2.919165e-03 8.150294e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147649 E001 268.038487 0.0148066021 1.308146e-03 5.004126e-03 8 97644184 97644545 362 + 2.220 2.135 -0.284
ENSG00000147649 E002 245.750654 0.0020865347 1.874112e-13 5.129966e-12 8 97644546 97644792 247 + 2.192 1.985 -0.691
ENSG00000147649 E003 194.139782 0.0014913966 7.169301e-13 1.800375e-11 8 97644793 97644887 95 + 2.092 1.871 -0.741
ENSG00000147649 E004 275.033325 0.0029358941 6.421251e-07 5.557525e-06 8 97661072 97661173 102 + 2.231 2.150 -0.271
ENSG00000147649 E005 350.203898 0.0001591455 1.333792e-11 2.722583e-10 8 97686668 97686752 85 + 2.335 2.264 -0.238
ENSG00000147649 E006 509.218260 0.0006119127 5.264027e-08 5.647925e-07 8 97687429 97687605 177 + 2.491 2.485 -0.017
ENSG00000147649 E007 280.406760 0.0005802937 7.472358e-04 3.068687e-03 8 97689038 97689103 66 + 2.229 2.254 0.085
ENSG00000147649 E008 313.454975 0.0002423319 2.779561e-06 2.096172e-05 8 97690952 97691018 67 + 2.281 2.271 -0.032
ENSG00000147649 E009 425.051473 0.0001918979 3.825564e-09 5.090550e-08 8 97691019 97691130 112 + 2.414 2.391 -0.074
ENSG00000147649 E010 379.749667 0.0001360450 4.205483e-06 3.042876e-05 8 97691131 97691188 58 + 2.362 2.372 0.035
ENSG00000147649 E011 359.784887 0.0039725661 1.358594e-02 3.727303e-02 8 97699754 97699852 99 + 2.335 2.373 0.127
ENSG00000147649 E012 3.890958 0.0040160512 9.892645e-01 9.973187e-01 8 97706412 97706625 214 + 0.518 0.622 0.472
ENSG00000147649 E013 400.443264 0.0026598697 5.952694e-04 2.515941e-03 8 97706626 97706750 125 + 2.384 2.397 0.044
ENSG00000147649 E014 346.062146 0.0007224236 1.104338e-02 3.126462e-02 8 97713662 97713769 108 + 2.316 2.378 0.209
ENSG00000147649 E015 450.289912 0.0003681564 1.113216e-05 7.318186e-05 8 97719049 97719189 141 + 2.434 2.457 0.077
ENSG00000147649 E016 533.213045 0.0003092747 1.640167e-04 8.093478e-04 8 97722879 97723035 157 + 2.505 2.556 0.170
ENSG00000147649 E017 3019.157986 0.0124623670 1.228912e-09 1.789911e-08 8 97724600 97730260 5661 + 3.212 3.555 1.141