ENSG00000147536

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000276533 ENSG00000147536 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS4 protein_coding protein_coding 36.47339 37.59731 41.80787 1.045384 3.974306 0.1531064 16.6897578 17.603288 17.51446235 0.5202147 0.26000785 -0.007294092 0.46698750 0.4681667 0.4265000000 -0.04166667 7.086032e-01 6.697512e-09 FALSE TRUE
ENST00000520736 ENSG00000147536 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS4 protein_coding retained_intron 36.47339 37.59731 41.80787 1.045384 3.974306 0.1531064 8.7707056 7.275589 12.18671391 0.8986144 3.84434405 0.743374902 0.22781250 0.1925667 0.2783000000 0.08573333 6.524920e-01 6.697512e-09   FALSE
MSTRG.31437.12 ENSG00000147536 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS4 protein_coding   36.47339 37.59731 41.80787 1.045384 3.974306 0.1531064 0.8020137 2.738315 0.03095905 1.4479503 0.03095905 -6.068221118 0.02454583 0.0741000 0.0007333333 -0.07336667 2.610059e-01 6.697512e-09 TRUE TRUE
MSTRG.31437.15 ENSG00000147536 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS4 protein_coding   36.47339 37.59731 41.80787 1.045384 3.974306 0.1531064 2.1802375 4.670331 1.13358350 0.5142951 0.08997412 -2.033048923 0.06236250 0.1248667 0.0275666667 -0.09730000 6.697512e-09 6.697512e-09 FALSE FALSE
MSTRG.31437.2 ENSG00000147536 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS4 protein_coding   36.47339 37.59731 41.80787 1.045384 3.974306 0.1531064 3.5251354 1.683413 6.28987181 0.8482154 0.83897056 1.895388595 0.08830417 0.0452000 0.1511000000 0.10590000 4.634006e-01 6.697512e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147536 E001 1.2072747 0.4123832723 9.561743e-01 9.765607e-01 8 41529215 41529217 3 + 0.365 0.347 -0.105
ENSG00000147536 E002 1.2072747 0.4123832723 9.561743e-01 9.765607e-01 8 41529218 41529220 3 + 0.365 0.347 -0.105
ENSG00000147536 E003 2.5242317 0.0742725528 3.201532e-01 4.632668e-01 8 41529221 41529226 6 + 0.424 0.623 0.953
ENSG00000147536 E004 3.0756744 0.0460412509 1.203781e-01 2.205134e-01 8 41529227 41529229 3 + 0.424 0.713 1.332
ENSG00000147536 E005 8.9005817 0.0316570217 6.561784e-01 7.658746e-01 8 41529230 41529236 7 + 1.012 0.971 -0.149
ENSG00000147536 E006 21.0880319 0.0090260551 2.964755e-01 4.376954e-01 8 41529237 41529248 12 + 1.377 1.312 -0.229
ENSG00000147536 E007 25.4987269 0.0089703509 2.334277e-01 3.664690e-01 8 41529249 41529260 12 + 1.460 1.387 -0.252
ENSG00000147536 E008 174.5615124 0.0060819343 2.348268e-01 3.681491e-01 8 41529261 41529388 128 + 2.259 2.228 -0.104
ENSG00000147536 E009 90.1890649 0.0025784905 7.631147e-01 8.465563e-01 8 41529389 41529396 8 + 1.937 1.977 0.134
ENSG00000147536 E010 7.5035521 0.0027644862 1.012072e-01 1.923206e-01 8 41529397 41530183 787 + 1.012 0.849 -0.612
ENSG00000147536 E011 224.9640351 0.0025614504 3.869549e-01 5.311168e-01 8 41530184 41530298 115 + 2.354 2.351 -0.010
ENSG00000147536 E012 14.3994862 0.0013811189 5.170256e-08 5.559280e-07 8 41531127 41531283 157 + 0.776 1.351 2.108
ENSG00000147536 E013 0.3299976 0.0274424043 2.286807e-01   8 41536358 41536359 2 + 0.000 0.214 10.315
ENSG00000147536 E014 181.6691035 0.0002360958 6.991566e-04 2.896094e-03 8 41536360 41536446 87 + 2.294 2.234 -0.199
ENSG00000147536 E015 0.4375944 0.0260073216 7.126574e-02 1.454285e-01 8 41536447 41536452 6 + 0.300 0.000 -11.821
ENSG00000147536 E016 179.9196010 0.0020283220 5.362013e-03 1.691535e-02 8 41537180 41537293 114 + 2.291 2.227 -0.215
ENSG00000147536 E017 6.0670942 0.0035503067 7.136409e-03 2.157768e-02 8 41537294 41538036 743 + 0.998 0.684 -1.222
ENSG00000147536 E018 144.7564803 0.0002218738 9.296023e-05 4.889374e-04 8 41539678 41539775 98 + 2.208 2.123 -0.285
ENSG00000147536 E019 156.7728573 0.0010205726 2.031307e-05 1.257680e-04 8 41539916 41540004 89 + 2.251 2.147 -0.349
ENSG00000147536 E020 10.9500974 0.0116448355 5.853597e-01 7.090767e-01 8 41541804 41541808 5 + 1.089 1.052 -0.134
ENSG00000147536 E021 204.5622222 0.0008581137 4.976687e-05 2.806091e-04 8 41541809 41541899 91 + 2.356 2.273 -0.277
ENSG00000147536 E022 2.6364632 0.0081175722 5.796122e-02 1.231379e-01 8 41541900 41541990 91 + 0.697 0.414 -1.316
ENSG00000147536 E023 195.0371470 0.0002154061 1.482337e-04 7.393613e-04 8 41541991 41542168 178 + 2.328 2.261 -0.222
ENSG00000147536 E024 31.0998897 0.0006496460 1.484147e-06 1.188164e-05 8 41542169 41542199 31 + 1.634 1.363 -0.932
ENSG00000147536 E025 545.3511244 0.0002774489 7.884335e-03 2.348786e-02 8 41542200 41543245 1046 + 2.744 2.731 -0.042
ENSG00000147536 E026 74.8961872 0.0003807647 6.228758e-01 7.395922e-01 8 41543246 41543248 3 + 1.851 1.899 0.164
ENSG00000147536 E027 462.7106379 0.0011048086 5.229565e-04 2.245575e-03 8 41543249 41543888 640 + 2.605 2.706 0.337
ENSG00000147536 E028 650.3013583 0.0026836424 4.130299e-17 1.859047e-15 8 41543889 41545030 1142 + 2.664 2.903 0.797
ENSG00000147536 E029 3.5587797 0.0047564227 1.093741e-02 3.101142e-02 8 41548332 41548376 45 + 0.822 0.465 -1.555
ENSG00000147536 E030 5.9763358 0.0266772520 1.075439e-01 2.019095e-01 8 41549561 41549666 106 + 0.952 0.759 -0.745
ENSG00000147536 E031 1.1029742 0.3562892193 5.763599e-01 7.018097e-01 8 41565206 41565324 119 + 0.220 0.368 1.011
ENSG00000147536 E032 1.4080945 0.1891837416 9.658907e-01 9.825453e-01 8 41566631 41566656 26 + 0.367 0.364 -0.019