ENSG00000147509

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000276500 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding protein_coding 4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 0.3747876 0.2561502 0.9731680 0.21386598 0.19463453 1.8851974 0.11386667 0.04006667 0.28083333 0.2407667 0.04574316 0.00130524 FALSE TRUE
ENST00000522225 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding protein_coding 4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 0.2584209 0.1864435 0.2475621 0.11828424 0.24756211 0.3908057 0.07420833 0.03046667 0.06506667 0.0346000 0.87140767 0.00130524 FALSE TRUE
ENST00000523064 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding processed_transcript 4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 1.1761914 1.9548545 0.7400103 0.49930880 0.12734150 -1.3894402 0.27216667 0.31630000 0.21006667 -0.1062333 0.59778841 0.00130524   FALSE
ENST00000523414 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding processed_transcript 4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 0.1884318 0.2233044 0.3638726 0.15752988 0.08197164 0.6803332 0.04322500 0.03683333 0.10583333 0.0690000 0.32969350 0.00130524 FALSE FALSE
MSTRG.31505.5 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding   4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 0.7282924 1.2959172 0.3045096 0.05229625 0.00583407 -2.0538877 0.17500417 0.20910000 0.08703333 -0.1220667 0.00130524 0.00130524   FALSE
MSTRG.31505.6 ENSG00000147509 HEK293_OSMI2_2hA HEK293_TMG_2hB RGS20 protein_coding   4.014056 6.210641 3.515245 0.1202512 0.16863 -0.8193399 1.1950891 2.2939713 0.7345502 0.53298575 0.23420285 -1.6296817 0.27989167 0.36723333 0.20410000 -0.1631333 0.40735244 0.00130524   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147509 E001 0.0000000       8 53851795 53851899 105 +      
ENSG00000147509 E002 0.1515154 0.0430543862 3.043022e-01   8 53851900 53852064 165 + 0.156 0.000 -10.485
ENSG00000147509 E003 0.0000000       8 53879258 53879408 151 +      
ENSG00000147509 E004 0.0000000       8 53879409 53879602 194 +      
ENSG00000147509 E005 0.0000000       8 53879603 53879831 229 +      
ENSG00000147509 E006 1.1780267 0.0121989077 1.968292e-02 5.081519e-02 8 53879832 53879883 52 + 0.000 0.434 13.062
ENSG00000147509 E007 6.9663048 0.0027065665 1.452501e-02 3.942207e-02 8 53879884 53879999 116 + 0.648 0.948 1.193
ENSG00000147509 E008 8.5945983 0.0032078298 1.647088e-02 4.377547e-02 8 53880000 53880038 39 + 0.759 1.025 1.018
ENSG00000147509 E009 22.8894339 0.0012911886 2.201101e-05 1.351325e-04 8 53880039 53880286 248 + 1.116 1.421 1.073
ENSG00000147509 E010 1.2576232 0.0193659966 6.088000e-01 7.284306e-01 8 53880872 53880893 22 + 0.270 0.349 0.517
ENSG00000147509 E011 2.7239175 0.0373292169 4.464516e-02 9.955029e-02 8 53880894 53880900 7 + 0.270 0.625 1.901
ENSG00000147509 E012 12.5917018 0.0053642375 9.234762e-01 9.557800e-01 8 53880901 53881084 184 + 1.116 1.099 -0.059
ENSG00000147509 E013 56.8770781 0.0005723874 9.766009e-16 3.691423e-14 8 53913497 53913922 426 + 1.481 1.816 1.138
ENSG00000147509 E014 6.5676092 0.0028324363 1.403094e-04 7.049033e-04 8 53939576 53939724 149 + 1.116 0.648 -1.808
ENSG00000147509 E015 0.0000000       8 53945325 53945370 46 +      
ENSG00000147509 E016 6.7170337 0.0027513802 4.833353e-04 2.096349e-03 8 53946665 53946748 84 + 1.101 0.671 -1.655
ENSG00000147509 E017 10.9691257 0.0019237137 5.479697e-11 1.010328e-09 8 53954076 53954310 235 + 1.385 0.734 -2.394
ENSG00000147509 E018 21.1074110 0.0127279433 8.309166e-15 2.766514e-13 8 53958270 53959303 1034 + 1.652 0.990 -2.323