ENSG00000147419

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356537 ENSG00000147419 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC25 protein_coding protein_coding 17.84235 7.858256 33.31553 0.423001 1.830163 2.082512 5.9979209 3.5069560 9.495484 0.71267185 0.1633723 1.434433 0.3868500 0.43980000 0.2866000 -0.15320000 0.15630608 0.01213193 FALSE TRUE
ENST00000519509 ENSG00000147419 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC25 protein_coding nonsense_mediated_decay 17.84235 7.858256 33.31553 0.423001 1.830163 2.082512 0.9518876 0.2143273 2.831832 0.21432731 0.3927790 3.663144 0.0422375 0.02866667 0.0862000 0.05753333 0.20932698 0.01213193 TRUE FALSE
ENST00000520202 ENSG00000147419 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC25 protein_coding nonsense_mediated_decay 17.84235 7.858256 33.31553 0.423001 1.830163 2.082512 5.5304086 3.2073511 9.353221 0.03730723 0.4861000 1.541131 0.3311625 0.40996667 0.2819333 -0.12803333 0.01213193 0.01213193   FALSE
ENST00000522915 ENSG00000147419 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC25 protein_coding protein_coding 17.84235 7.858256 33.31553 0.423001 1.830163 2.082512 3.9386267 0.3129780 9.695918 0.31297797 1.3066210 4.909357 0.1682792 0.04233333 0.2894000 0.24706667 0.06042351 0.01213193 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147419 E001 0.0000000       8 27733316 27733317 2 -      
ENSG00000147419 E002 242.6545840 0.0054183418 3.206947e-06 2.383938e-05 8 27733318 27735051 1734 - 2.277 2.485 0.692
ENSG00000147419 E003 331.6592063 0.0003327362 8.243267e-14 2.373347e-12 8 27735052 27735812 761 - 2.427 2.598 0.571
ENSG00000147419 E004 69.0917553 0.0015358274 1.967927e-01 3.222403e-01 8 27735813 27735861 49 - 1.777 1.861 0.283
ENSG00000147419 E005 118.7789148 0.0038026739 6.927280e-02 1.421526e-01 8 27735862 27736051 190 - 2.007 2.105 0.328
ENSG00000147419 E006 43.1628671 0.0178957661 4.140798e-01 5.574919e-01 8 27736052 27736077 26 - 1.580 1.663 0.285
ENSG00000147419 E007 81.9810497 0.0089898484 6.623775e-01 7.706956e-01 8 27736078 27736162 85 - 1.865 1.908 0.143
ENSG00000147419 E008 102.5429829 0.0003477645 1.167164e-02 3.275108e-02 8 27736163 27736245 83 - 1.996 1.920 -0.254
ENSG00000147419 E009 87.8102513 0.0003978342 4.247690e-04 1.869293e-03 8 27740472 27740517 46 - 1.944 1.815 -0.434
ENSG00000147419 E010 0.4375944 0.0265047953 4.087861e-01 5.524005e-01 8 27747753 27747876 124 - 0.195 0.000 -10.514
ENSG00000147419 E011 0.5848434 0.0248081234 2.614620e-01 3.990975e-01 8 27747877 27748071 195 - 0.245 0.000 -10.936
ENSG00000147419 E012 0.5827671 0.1266338498 2.993575e-01 4.408680e-01 8 27748072 27748076 5 - 0.244 0.000 -10.172
ENSG00000147419 E013 189.6475799 0.0004146627 2.217944e-08 2.564436e-07 8 27748077 27748279 203 - 2.277 2.133 -0.481
ENSG00000147419 E014 0.2934659 0.0284793623 4.511630e-01   8 27748280 27748475 196 - 0.075 0.193 1.562
ENSG00000147419 E015 128.4640658 0.0003088659 4.713330e-05 2.672552e-04 8 27748495 27748598 104 - 2.104 1.983 -0.407
ENSG00000147419 E016 114.8090080 0.0004648158 8.040393e-06 5.456862e-05 8 27752512 27752587 76 - 2.060 1.912 -0.500
ENSG00000147419 E017 0.0000000       8 27752588 27752589 2 -      
ENSG00000147419 E018 66.8227823 0.0004737896 6.989139e-04 2.895221e-03 8 27756719 27756770 52 - 1.828 1.681 -0.495
ENSG00000147419 E019 73.8957055 0.0003848015 4.639324e-02 1.027467e-01 8 27762419 27762458 40 - 1.853 1.784 -0.230
ENSG00000147419 E020 0.9922594 0.0132064568 7.029183e-01 8.016673e-01 8 27764286 27764533 248 - 0.245 0.326 0.565
ENSG00000147419 E021 70.2833569 0.0004080817 2.385187e-02 5.950153e-02 8 27765204 27765251 48 - 1.835 1.751 -0.283
ENSG00000147419 E022 64.7587380 0.0003738611 2.948631e-03 1.011362e-02 8 27772513 27772653 141 - 1.811 1.686 -0.424