ENSG00000147274

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000320676 ENSG00000147274 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX protein_coding protein_coding 259.5824 208.5373 356.3957 25.45151 12.52263 0.7731462 167.94604 163.578941 203.250084 20.911483 8.756413 0.3132517 0.67249167 0.78346667 0.57056667 -0.21290000 3.478899e-08 1.994594e-26 FALSE TRUE
ENST00000419968 ENSG00000147274 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX protein_coding protein_coding 259.5824 208.5373 356.3957 25.45151 12.52263 0.7731462 17.67637 0.000000 24.570586 0.000000 6.614843 11.2633036 0.05081667 0.00000000 0.06840000 0.06840000 1.128027e-24 1.994594e-26 FALSE TRUE
ENST00000496459 ENSG00000147274 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX protein_coding processed_transcript 259.5824 208.5373 356.3957 25.45151 12.52263 0.7731462 10.83332 22.745716 6.336693 8.086867 3.275455 -1.8421520 0.05204167 0.10350000 0.01723333 -0.08626667 3.750332e-01 1.994594e-26 TRUE TRUE
MSTRG.34962.6 ENSG00000147274 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX protein_coding   259.5824 208.5373 356.3957 25.45151 12.52263 0.7731462 32.04405 6.019075 70.636584 3.009539 4.131284 3.5506112 0.10099167 0.03286667 0.19786667 0.16500000 3.648198e-01 1.994594e-26 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147274 E001 25.4457232 2.394864e-02 1.995336e-01 3.256481e-01 X 136848004 136848582 579 - 1.358 1.480 0.423
ENSG00000147274 E002 0.2934659 2.821939e-02 7.438029e-01   X 136869187 136869191 5 - 0.100 0.149 0.654
ENSG00000147274 E003 21.4029976 5.602168e-02 2.204892e-01 3.509963e-01 X 136869192 136869193 2 - 1.404 1.285 -0.414
ENSG00000147274 E004 101.4227429 1.193956e-02 4.443960e-01 5.859717e-01 X 136869194 136869258 65 - 1.983 2.049 0.221
ENSG00000147274 E005 298.7860127 1.005341e-02 7.668364e-01 8.492229e-01 X 136869259 136869358 100 - 2.464 2.491 0.091
ENSG00000147274 E006 1571.7840640 5.345033e-03 1.440721e-01 2.535465e-01 X 136869359 136869971 613 - 3.215 3.164 -0.170
ENSG00000147274 E007 1329.6090650 5.742237e-03 4.238684e-09 5.591963e-08 X 136869972 136870417 446 - 3.205 2.977 -0.759
ENSG00000147274 E008 303.6922586 2.247739e-03 1.238092e-18 6.755554e-17 X 136872268 136872274 7 - 2.583 2.295 -0.963
ENSG00000147274 E009 483.2139565 2.923363e-03 7.589686e-18 3.780813e-16 X 136872275 136872345 71 - 2.783 2.501 -0.937
ENSG00000147274 E010 290.8172020 1.708714e-02 1.600191e-03 5.961798e-03 X 136872346 136873245 900 - 2.546 2.316 -0.768
ENSG00000147274 E011 495.1526352 6.442997e-04 6.292498e-58 5.960475e-55 X 136873246 136873448 203 - 2.812 2.464 -1.159
ENSG00000147274 E012 183.9637743 1.687791e-03 2.043383e-14 6.426175e-13 X 136873449 136873460 12 - 2.361 2.093 -0.897
ENSG00000147274 E013 2841.9460867 2.385336e-03 4.873933e-10 7.645601e-09 X 136873461 136873957 497 - 3.383 3.526 0.473
ENSG00000147274 E014 684.5628400 2.072141e-03 2.845491e-18 1.480554e-16 X 136873958 136873977 20 - 2.723 2.949 0.752
ENSG00000147274 E015 4100.5108425 1.488691e-04 2.315782e-55 1.976207e-52 X 136873978 136874452 475 - 3.559 3.675 0.385
ENSG00000147274 E016 207.2382853 5.592853e-03 1.918011e-27 2.589889e-25 X 136874453 136875085 633 - 2.474 1.944 -1.771
ENSG00000147274 E017 2015.9552220 5.680027e-04 5.687806e-01 6.954011e-01 X 136875086 136875168 83 - 3.301 3.306 0.018
ENSG00000147274 E018 2140.0056458 1.830186e-04 3.832252e-01 5.274099e-01 X 136875258 136875383 126 - 3.326 3.335 0.028
ENSG00000147274 E019 1824.0563106 1.455695e-04 7.441381e-02 1.505979e-01 X 136875471 136875585 115 - 3.253 3.271 0.059
ENSG00000147274 E020 1590.0566657 6.525496e-05 9.879290e-01 9.965020e-01 X 136876503 136876599 97 - 3.201 3.203 0.004
ENSG00000147274 E021 1438.2471383 4.927945e-04 2.652018e-01 4.032367e-01 X 136876600 136876655 56 - 3.165 3.147 -0.062
ENSG00000147274 E022 977.3810633 2.652575e-03 9.786933e-01 9.907763e-01 X 136877915 136877915 1 - 2.991 2.986 -0.016
ENSG00000147274 E023 1514.9046525 9.828243e-04 5.775804e-01 7.028124e-01 X 136877916 136877964 49 - 3.177 3.182 0.017
ENSG00000147274 E024 1838.8487801 4.252715e-04 6.996802e-01 7.993217e-01 X 136877965 136878086 122 - 3.267 3.259 -0.029
ENSG00000147274 E025 2124.4671800 3.934015e-04 5.837027e-04 2.473786e-03 X 136879017 136879123 107 - 3.345 3.301 -0.147
ENSG00000147274 E026 6.4771651 3.862892e-03 8.587974e-01 9.129091e-01 X 136879124 136879158 35 - 0.874 0.853 -0.081
ENSG00000147274 E027 0.4815130 2.199673e-02 3.392552e-01 4.831548e-01 X 136879159 136879198 40 - 0.100 0.259 1.654
ENSG00000147274 E028 0.4751703 2.058616e-02 3.383128e-01 4.821706e-01 X 136879199 136879318 120 - 0.100 0.259 1.655
ENSG00000147274 E029 2289.4371483 1.409612e-03 2.908217e-02 7.016418e-02 X 136879319 136879453 135 - 3.378 3.328 -0.166
ENSG00000147274 E030 1264.6525743 4.218502e-03 3.256292e-01 4.690715e-01 X 136880597 136880771 175 - 3.118 3.074 -0.147