ENSG00000147257

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370818 ENSG00000147257 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC3 protein_coding protein_coding 55.10201 76.59536 38.424 5.330299 0.3979755 -0.9950624 30.288095 41.817902 20.77061 2.040601 0.2142640 -1.0092276 0.54852083 0.5510333 0.5407333 -0.0103000 0.9606008914 0.0006486107 FALSE TRUE
ENST00000406757 ENSG00000147257 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC3 protein_coding protein_coding 55.10201 76.59536 38.424 5.330299 0.3979755 -0.9950624 22.270120 26.483564 17.16733 1.522819 0.5516673 -0.6251358 0.41630417 0.3465000 0.4466333 0.1001333 0.0006486107 0.0006486107 FALSE TRUE
ENST00000669691 ENSG00000147257 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC3 protein_coding processed_transcript 55.10201 76.59536 38.424 5.330299 0.3979755 -0.9950624 1.935203 7.745019 0.00000 4.899305 0.0000000 -9.5989864 0.02362083 0.0950000 0.0000000 -0.0950000 0.2066749773 0.0006486107 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000147257 E001 315.2078860 0.0027703314 3.643696e-14 1.103400e-12 X 133535745 133536012 268 - 2.226 2.498 0.907
ENSG00000147257 E002 306.0058161 0.0035029707 4.634706e-16 1.826948e-14 X 133536013 133536123 111 - 2.175 2.491 1.056
ENSG00000147257 E003 192.0060709 0.0011667659 2.466567e-08 2.824491e-07 X 133536124 133536136 13 - 2.076 2.273 0.658
ENSG00000147257 E004 200.6797567 0.0020242599 7.015766e-07 6.022274e-06 X 133536137 133536148 12 - 2.103 2.290 0.626
ENSG00000147257 E005 584.9196458 0.0032732852 2.717260e-03 9.424866e-03 X 133536149 133536293 145 - 2.636 2.732 0.320
ENSG00000147257 E006 724.5194060 0.0014132958 4.005855e-02 9.113863e-02 X 133596440 133596599 160 - 2.768 2.815 0.157
ENSG00000147257 E007 523.6855267 0.0001189921 1.316762e-01 2.364743e-01 X 133661730 133661850 121 - 2.641 2.670 0.098
ENSG00000147257 E008 0.4815130 0.0205184174 8.644820e-01 9.166446e-01 X 133661851 133661941 91 - 0.178 0.152 -0.280
ENSG00000147257 E009 0.1482932 0.0410135697 1.000000e+00   X 133661942 133662111 170 - 0.000 0.082 9.167
ENSG00000147257 E010 0.0000000       X 133692113 133692368 256 -      
ENSG00000147257 E011 449.1285397 0.0002676245 7.853565e-03 2.341424e-02 X 133692369 133692494 126 - 2.631 2.583 -0.160
ENSG00000147257 E012 1.4350757 0.0322502229 3.461676e-01 4.903282e-01 X 133692495 133692514 20 - 0.481 0.310 -0.960
ENSG00000147257 E013 464.1617418 0.0010142055 4.069539e-04 1.801481e-03 X 133699895 133700028 134 - 2.666 2.587 -0.262
ENSG00000147257 E014 0.6966155 0.0172671820 8.686733e-01 9.196030e-01 X 133700617 133700709 93 - 0.178 0.211 0.307
ENSG00000147257 E015 0.0000000       X 133702617 133702694 78 -      
ENSG00000147257 E016 0.0000000       X 133703008 133703106 99 -      
ENSG00000147257 E017 2.4691523 0.0062678156 5.290562e-02 1.143139e-01 X 133704205 133704273 69 - 0.704 0.392 -1.470
ENSG00000147257 E018 986.2635156 0.0019815218 1.075869e-05 7.096799e-05 X 133753482 133754043 562 - 3.004 2.909 -0.317
ENSG00000147257 E019 315.6368318 0.0020460142 4.331718e-04 1.901942e-03 X 133754044 133754176 133 - 2.517 2.412 -0.351
ENSG00000147257 E020 0.0000000       X 133834500 133834570 71 -      
ENSG00000147257 E021 174.7771565 0.0020711659 3.555718e-03 1.188504e-02 X 133953050 133953097 48 - 2.260 2.156 -0.348
ENSG00000147257 E022 225.2890568 0.0006018631 1.407863e-08 1.688928e-07 X 133953098 133953211 114 - 2.404 2.253 -0.502
ENSG00000147257 E023 236.7314884 0.0023661281 1.093421e-08 1.338408e-07 X 133985275 133985535 261 - 2.449 2.261 -0.628
ENSG00000147257 E024 73.8881276 0.0049070923 9.732353e-04 3.867525e-03 X 133985536 133985617 82 - 1.940 1.760 -0.607
ENSG00000147257 E025 0.1817044 0.0408367611 1.000000e+00   X 133987057 133987100 44 - 0.000 0.082 9.171
ENSG00000147257 E026 0.5149111 0.0203982116 8.662404e-01 9.178738e-01 X 134000376 134000439 64 - 0.178 0.152 -0.278
ENSG00000147257 E027 0.0000000       X 134023124 134023209 86 -