ENSG00000146950

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380913 ENSG00000146950 HEK293_OSMI2_2hA HEK293_TMG_2hB SHROOM2 protein_coding protein_coding 0.3667778 0.6863983 0.1251507 0.06064461 0.02588705 -2.365344 0.2179939 0.2930535 0.1251507 0.08135200 0.02588705 -1.165004 0.7101458 0.4115 1 0.5885 0.004946990 0.00494699 FALSE TRUE
ENST00000418909 ENSG00000146950 HEK293_OSMI2_2hA HEK293_TMG_2hB SHROOM2 protein_coding protein_coding 0.3667778 0.6863983 0.1251507 0.06064461 0.02588705 -2.365344 0.1366764 0.3933447 0.0000000 0.02483216 0.00000000 -5.333942 0.2600333 0.5885 0 -0.5885 0.004965206 0.00494699 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146950 E001 0.7707125 0.0158897429 0.20777840 0.33580442 X 9786429 9786710 282 + 0.393 0.155 -1.776
ENSG00000146950 E002 2.2455145 0.0068388384 0.19555137 0.32065002 X 9873652 9873803 152 + 0.596 0.361 -1.188
ENSG00000146950 E003 1.8433971 0.0109663333 0.02406559 0.05994954 X 9890977 9891108 132 + 0.671 0.270 -2.095
ENSG00000146950 E004 13.2161132 0.0378081975 0.03085399 0.07363191 X 9894358 9896698 2341 + 1.197 0.953 -0.887
ENSG00000146950 E005 0.0000000       X 9898145 9898189 45 +      
ENSG00000146950 E006 2.2444677 0.0074588956 0.41674044 0.56012533 X 9898190 9898290 101 + 0.240 0.412 1.103
ENSG00000146950 E007 0.0000000       X 9912434 9912587 154 +      
ENSG00000146950 E008 0.0000000       X 9912683 9913227 545 +      
ENSG00000146950 E009 5.4758077 0.0034072504 0.30568897 0.44781134 X 9932175 9932870 696 + 0.507 0.689 0.813
ENSG00000146950 E010 6.8482257 0.0029272806 0.52660426 0.65979907 X 9937134 9937685 552 + 0.671 0.770 0.407
ENSG00000146950 E011 3.2302958 0.0052561196 0.97254709 0.98687319 X 9939195 9939366 172 + 0.507 0.499 -0.036
ENSG00000146950 E012 0.8803962 0.0136968632 0.82965339 0.89304173 X 9944641 9944643 3 + 0.240 0.197 -0.358
ENSG00000146950 E013 3.4526093 0.0138130103 0.01481280 0.04007205 X 9944644 9944913 270 + 0.001 0.587 10.541
ENSG00000146950 E014 44.2156774 0.0007707119 0.09092053 0.17659757 X 9946671 9949443 2773 + 1.416 1.506 0.310