ENSG00000146828

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354161 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding protein_coding 23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 5.6602944 8.5366438 2.6089707 1.06090314 1.0691156 -1.7063581 0.23757917 0.2785333 0.1691667 -0.10936667 4.749971e-01 1.710535e-14 FALSE  
ENST00000475623 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding processed_transcript 23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 0.7140573 0.0000000 0.9672886 0.00000000 0.1911359 6.6107128 0.03740417 0.0000000 0.0627000 0.06270000 1.710535e-14 1.710535e-14 FALSE  
ENST00000475687 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding retained_intron 23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 1.4487065 1.5505176 0.9443459 0.42881997 0.3363370 -0.7094406 0.06315000 0.0485000 0.0609000 0.01240000 9.291335e-01 1.710535e-14 FALSE  
ENST00000497958 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding retained_intron 23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 1.5261916 0.8344628 1.1110715 0.02894746 0.3107535 0.4087726 0.07265417 0.0270000 0.0726000 0.04560000 7.407129e-02 1.710535e-14 FALSE  
MSTRG.30355.13 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding   23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 1.4201823 3.2230813 1.2146484 0.45823746 0.2446877 -1.4005422 0.05861250 0.1040667 0.0789000 -0.02516667 6.374734e-01 1.710535e-14 FALSE  
MSTRG.30355.2 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding   23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 5.0481982 6.8710134 2.8928850 0.81047859 1.0103098 -1.2451336 0.21186250 0.2197333 0.1883000 -0.03143333 8.105747e-01 1.710535e-14 FALSE  
MSTRG.30355.6 ENSG00000146828 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC12A9 protein_coding   23.11493 31.07564 15.38329 2.109023 0.3728743 -1.013947 1.6776709 3.2393341 0.8704950 0.63887785 0.4367756 -1.8837573 0.06929167 0.1056667 0.0571000 -0.04856667 4.273768e-01 1.710535e-14 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146828 E001 10.5227257 0.0024638226 5.658056e-01 6.928464e-01 7 100826820 100827047 228 + 1.026 0.982 -0.163
ENSG00000146828 E002 0.2214452 0.0373018168 1.000000e+00   7 100852711 100852712 2 + 0.000 0.083 9.449
ENSG00000146828 E003 0.2214452 0.0373018168 1.000000e+00   7 100852713 100852718 6 + 0.000 0.083 9.449
ENSG00000146828 E004 0.3697384 0.0274424043 5.983108e-01 7.199361e-01 7 100852719 100852719 1 + 0.000 0.152 10.531
ENSG00000146828 E005 1.1887125 0.0176738686 1.169140e-01 2.155628e-01 7 100852720 100852721 2 + 0.481 0.212 -1.688
ENSG00000146828 E006 6.5904403 0.0025808476 6.528697e-01 7.634958e-01 7 100852722 100852732 11 + 0.850 0.809 -0.158
ENSG00000146828 E007 9.9097853 0.0019699120 2.667338e-01 4.049782e-01 7 100852733 100852736 4 + 1.046 0.942 -0.386
ENSG00000146828 E008 15.5088239 0.0011604681 4.488681e-01 5.901005e-01 7 100852737 100852745 9 + 1.195 1.145 -0.178
ENSG00000146828 E009 41.7073486 0.0007693585 1.304335e-01 2.347639e-01 7 100852746 100852776 31 + 1.623 1.555 -0.231
ENSG00000146828 E010 53.9931439 0.0004207780 1.246769e-01 2.266740e-01 7 100852777 100852835 59 + 1.721 1.663 -0.199
ENSG00000146828 E011 23.2200466 0.0008428497 4.970094e-01 6.335799e-01 7 100852836 100852839 4 + 1.348 1.315 -0.112
ENSG00000146828 E012 0.8899480 0.0151717006 5.753551e-01 7.009774e-01 7 100853286 100853446 161 + 0.304 0.212 -0.688
ENSG00000146828 E013 114.2613917 0.0008502335 2.163324e-01 3.459838e-01 7 100854156 100854378 223 + 2.016 1.991 -0.085
ENSG00000146828 E014 103.1478053 0.0035446577 8.361609e-01 8.975578e-01 7 100854620 100854754 135 + 1.945 1.958 0.045
ENSG00000146828 E015 7.4768796 0.1314999480 1.475081e-01 2.583024e-01 7 100855441 100855705 265 + 1.064 0.777 -1.085
ENSG00000146828 E016 114.4368556 0.0087583925 2.991710e-01 4.406721e-01 7 100855706 100855837 132 + 2.029 1.993 -0.120
ENSG00000146828 E017 45.4397201 0.0192875688 9.285789e-05 4.884398e-04 7 100855838 100856793 956 + 1.801 1.503 -1.014
ENSG00000146828 E018 11.8803673 0.0037492952 9.600987e-01 9.789089e-01 7 100856794 100856867 74 + 1.026 1.052 0.095
ENSG00000146828 E019 141.7833975 0.0048334940 6.772156e-01 7.819904e-01 7 100856868 100857029 162 + 2.088 2.094 0.018
ENSG00000146828 E020 152.1620605 0.0028982144 3.212728e-01 4.645056e-01 7 100857030 100857176 147 + 2.134 2.117 -0.056
ENSG00000146828 E021 32.0241203 0.0010879972 1.104472e-08 1.350800e-07 7 100857177 100858735 1559 + 1.682 1.341 -1.169
ENSG00000146828 E022 6.4712073 0.0026941130 9.102862e-02 1.767586e-01 7 100858736 100858834 99 + 0.959 0.764 -0.751
ENSG00000146828 E023 175.6384108 0.0005119385 5.539001e-03 1.738855e-02 7 100858835 100858942 108 + 2.228 2.166 -0.207
ENSG00000146828 E024 0.9201370 0.0141529281 6.795360e-01 7.836809e-01 7 100858943 100859036 94 + 0.178 0.264 0.727
ENSG00000146828 E025 0.6568746 0.0211273374 9.006042e-01 9.405814e-01 7 100859037 100859049 13 + 0.178 0.211 0.309
ENSG00000146828 E026 164.4443522 0.0002356129 1.299701e-02 3.590376e-02 7 100859050 100859161 112 + 2.196 2.143 -0.177
ENSG00000146828 E027 6.2127423 0.0026592620 1.682020e-04 8.274781e-04 7 100859162 100859884 723 + 1.084 0.638 -1.734
ENSG00000146828 E028 206.1264100 0.0002536761 2.825368e-01 4.225406e-01 7 100859885 100860042 158 + 2.256 2.248 -0.025
ENSG00000146828 E029 3.3463262 0.0047884709 2.641000e-01 4.020438e-01 7 100860043 100860149 107 + 0.703 0.545 -0.690
ENSG00000146828 E030 135.2577298 0.0053062894 2.455104e-01 3.806499e-01 7 100860150 100860232 83 + 2.009 2.091 0.274
ENSG00000146828 E031 19.2206443 0.0013233590 2.182558e-05 1.341115e-04 7 100860233 100860516 284 + 1.452 1.132 -1.121
ENSG00000146828 E032 43.0147083 0.0005111108 7.976843e-17 3.473998e-15 7 100860517 100861011 495 + 1.853 1.427 -1.451
ENSG00000146828 E033 20.7279915 0.0047926409 1.023651e-04 5.329733e-04 7 100861012 100861137 126 + 1.475 1.177 -1.040
ENSG00000146828 E034 166.4113965 0.0039867849 3.342588e-02 7.863055e-02 7 100861138 100861262 125 + 2.070 2.189 0.399
ENSG00000146828 E035 10.4468505 0.0016086438 6.770967e-03 2.062813e-02 7 100861263 100861391 129 + 1.181 0.920 -0.953
ENSG00000146828 E036 193.9844955 0.0015342765 8.316133e-01 8.944673e-01 7 100861392 100861519 128 + 2.215 2.232 0.057
ENSG00000146828 E037 128.1197758 0.0002948136 6.778991e-01 7.825030e-01 7 100861520 100861584 65 + 2.041 2.048 0.025
ENSG00000146828 E038 197.8688356 0.0002225777 6.541315e-01 7.644015e-01 7 100861737 100861911 175 + 2.208 2.243 0.117
ENSG00000146828 E039 230.5007002 0.0001848810 9.968729e-03 2.868692e-02 7 100862681 100862827 147 + 2.229 2.321 0.306
ENSG00000146828 E040 4.7241274 0.0034083117 7.929644e-01 8.676138e-01 7 100865287 100865359 73 + 0.657 0.715 0.240
ENSG00000146828 E041 4.9423650 0.0032918672 9.430286e-01 9.682668e-01 7 100865360 100865718 359 + 0.703 0.732 0.117
ENSG00000146828 E042 829.4264913 0.0024469387 1.236541e-11 2.536368e-10 7 100865719 100867042 1324 + 2.714 2.899 0.617