ENSG00000146757

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000328747 ENSG00000146757 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF92 protein_coding protein_coding 6.577007 1.606607 10.60046 0.2212616 0.3786137 2.714447 4.71854818 0.6525407 8.0073421 0.19377043 0.2904073 3.5970430 0.64955417 0.4042333 0.75616667 3.519333e-01 3.123535e-02 3.944701e-08 FALSE TRUE
ENST00000357512 ENSG00000146757 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF92 protein_coding protein_coding 6.577007 1.606607 10.60046 0.2212616 0.3786137 2.714447 0.49791084 0.2245035 1.1742399 0.22450346 0.2263653 2.3362802 0.08435000 0.1095667 0.10956667 1.387779e-17 6.177610e-01 3.944701e-08 FALSE TRUE
ENST00000431504 ENSG00000146757 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF92 protein_coding protein_coding 6.577007 1.606607 10.60046 0.2212616 0.3786137 2.714447 0.79551099 0.6100557 0.8808662 0.21501772 0.1174620 0.5228108 0.16032917 0.4125000 0.08323333 -3.292667e-01 6.547646e-02 3.944701e-08 FALSE TRUE
ENST00000450302 ENSG00000146757 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF92 protein_coding protein_coding 6.577007 1.606607 10.60046 0.2212616 0.3786137 2.714447 0.48275348 0.0000000 0.5380143 0.00000000 0.1017503 5.7761416 0.08328333 0.0000000 0.05103333 5.103333e-02 1.323110e-02 3.944701e-08 FALSE TRUE
ENST00000488849 ENSG00000146757 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF92 protein_coding retained_intron 6.577007 1.606607 10.60046 0.2212616 0.3786137 2.714447 0.08228373 0.1195066 0.0000000 0.02184502 0.0000000 -3.6949535 0.02247083 0.0737000 0.00000000 -7.370000e-02 3.944701e-08 3.944701e-08   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146757 E001 2.1453732 0.0428214656 8.294033e-01 8.928810e-01 7 65373799 65373837 39 + 0.387 0.442 0.296
ENSG00000146757 E002 1.9970800 0.0552417272 6.161693e-01 7.342707e-01 7 65373838 65373838 1 + 0.387 0.276 -0.699
ENSG00000146757 E003 3.0280507 0.0053001341 2.687636e-01 4.072503e-01 7 65373839 65373854 16 + 0.516 0.275 -1.367
ENSG00000146757 E004 13.0530669 0.0082285973 2.288707e-01 3.611042e-01 7 65373855 65373880 26 + 1.020 0.857 -0.613
ENSG00000146757 E005 39.9438300 0.0005591230 9.817580e-03 2.831346e-02 7 65373881 65374000 120 + 1.483 1.271 -0.734
ENSG00000146757 E006 41.5451353 0.0005008558 1.093273e-03 4.281139e-03 7 65387902 65388028 127 + 1.504 1.228 -0.961
ENSG00000146757 E007 0.8417882 0.0156123704 1.204848e-02 3.364953e-02 7 65388029 65388320 292 + 0.093 0.562 3.465
ENSG00000146757 E008 31.5702146 0.0036514914 1.568169e-03 5.855968e-03 7 65388806 65388901 96 + 1.395 1.064 -1.170
ENSG00000146757 E009 385.7137617 0.0012024549 7.254484e-08 7.567536e-07 7 65398341 65401136 2796 + 2.417 2.500 0.278