ENSG00000146535

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000275364 ENSG00000146535 HEK293_OSMI2_2hA HEK293_TMG_2hB GNA12 protein_coding protein_coding 26.14473 33.2358 25.18637 2.293507 0.6032739 -0.3999564 7.068443 10.456426 5.681907 0.6145118 0.2688341 -0.8787851 0.2640083 0.3151333 0.2259000 -0.08923333 0.0028556744 0.0002864753 FALSE TRUE
ENST00000491117 ENSG00000146535 HEK293_OSMI2_2hA HEK293_TMG_2hB GNA12 protein_coding processed_transcript 26.14473 33.2358 25.18637 2.293507 0.6032739 -0.3999564 10.648878 17.139378 7.449757 1.0464265 0.5965737 -1.2009558 0.3996125 0.5179333 0.2968000 -0.22113333 0.0003901006 0.0002864753 FALSE TRUE
MSTRG.29407.1 ENSG00000146535 HEK293_OSMI2_2hA HEK293_TMG_2hB GNA12 protein_coding   26.14473 33.2358 25.18637 2.293507 0.6032739 -0.3999564 6.925220 4.392915 9.513342 1.3933578 0.3154047 1.1130090 0.2742458 0.1285333 0.3787333 0.25020000 0.0002864753 0.0002864753 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146535 E001 3.7743793 0.0192266619 2.880142e-01 4.285569e-01 7 2727956 2727999 44 - 0.535 0.716 0.792
ENSG00000146535 E002 50.7735833 0.0006353911 7.468273e-07 6.375172e-06 7 2728105 2728560 456 - 1.490 1.774 0.965
ENSG00000146535 E003 18.2676369 0.0125696043 5.559017e-02 1.189880e-01 7 2728561 2728565 5 - 1.118 1.322 0.719
ENSG00000146535 E004 556.7868724 0.0010164895 1.856718e-13 5.086027e-12 7 2728566 2729255 690 - 2.610 2.774 0.544
ENSG00000146535 E005 584.4722398 0.0012875182 9.844460e-06 6.555833e-05 7 2729256 2729823 568 - 2.674 2.779 0.351
ENSG00000146535 E006 1145.4557435 0.0005356806 1.684398e-03 6.233355e-03 7 2729824 2731022 1199 - 3.003 3.054 0.171
ENSG00000146535 E007 462.1844017 0.0002165394 2.491906e-04 1.171745e-03 7 2731023 2731333 311 - 2.676 2.624 -0.174
ENSG00000146535 E008 127.4228197 0.0003398729 2.979113e-01 4.392968e-01 7 2731334 2731350 17 - 2.101 2.077 -0.081
ENSG00000146535 E009 214.8334040 0.0010996525 3.943648e-01 5.382183e-01 7 2731351 2731435 85 - 2.319 2.305 -0.045
ENSG00000146535 E010 321.6804574 0.0008199662 2.513454e-04 1.180822e-03 7 2731436 2731737 302 - 2.534 2.460 -0.247
ENSG00000146535 E011 96.2872833 0.0003653837 5.148100e-04 2.214761e-03 7 2731738 2731750 13 - 2.037 1.919 -0.396
ENSG00000146535 E012 0.9350151 0.2856530718 9.228149e-02 1.786896e-01 7 2732628 2732724 97 - 0.482 0.102 -2.945
ENSG00000146535 E013 153.2033106 0.0003303687 5.006716e-06 3.563540e-05 7 2733451 2733501 51 - 2.242 2.117 -0.415
ENSG00000146535 E014 0.0000000       7 2760342 2760452 111 -      
ENSG00000146535 E015 0.0000000       7 2762170 2762393 224 -      
ENSG00000146535 E016 0.0000000       7 2762592 2763075 484 -      
ENSG00000146535 E017 0.0000000       7 2763076 2763079 4 -      
ENSG00000146535 E018 0.0000000       7 2763080 2763327 248 -      
ENSG00000146535 E019 4.8630341 0.0071736600 6.761233e-01 7.811820e-01 7 2794720 2794927 208 - 0.767 0.716 -0.207
ENSG00000146535 E020 151.0616678 0.0002700302 4.267844e-07 3.827362e-06 7 2794928 2794966 39 - 2.243 2.104 -0.463
ENSG00000146535 E021 236.9184532 0.0022628886 5.663778e-06 3.981837e-05 7 2794967 2795143 177 - 2.434 2.303 -0.437
ENSG00000146535 E022 0.2998086 0.0303036753 7.383368e-01   7 2795144 2795145 2 - 0.148 0.101 -0.629
ENSG00000146535 E023 0.3332198 0.0301602116 7.389696e-01   7 2814312 2814389 78 - 0.148 0.101 -0.626
ENSG00000146535 E024 0.0000000       7 2814877 2815259 383 -      
ENSG00000146535 E025 1.0748892 0.0331949851 9.135929e-02 1.772482e-01 7 2839073 2839125 53 - 0.480 0.183 -1.950
ENSG00000146535 E026 1.8518150 0.1606167437 5.553590e-01 6.842375e-01 7 2843818 2843852 35 - 0.345 0.488 0.776
ENSG00000146535 E027 128.6452230 0.0018834264 6.188978e-10 9.546355e-09 7 2843853 2844308 456 - 2.218 2.001 -0.727