ENSG00000146376

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368149 ENSG00000146376 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGAP18 protein_coding protein_coding 2.473928 0.5238759 3.648039 0.06868082 0.4529115 2.776494 1.1576613 0.5238759 1.5512988 0.06868082 0.13080342 1.548170 0.6503792 1 0.4326333 -0.5673667 8.660354e-10 8.660354e-10 FALSE TRUE
ENST00000483367 ENSG00000146376 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGAP18 protein_coding processed_transcript 2.473928 0.5238759 3.648039 0.06868082 0.4529115 2.776494 0.3834631 0.0000000 0.5502042 0.00000000 0.28086258 5.807881 0.1079875 0 0.1355667 0.1355667 4.988321e-01 8.660354e-10   FALSE
MSTRG.28954.4 ENSG00000146376 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGAP18 protein_coding   2.473928 0.5238759 3.648039 0.06868082 0.4529115 2.776494 0.9328038 0.0000000 1.5465365 0.00000000 0.08249769 7.282196 0.2416417 0 0.4318333 0.4318333 1.298834e-07 8.660354e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146376 E001 50.5919611 0.0006363068 1.222591e-09 1.781253e-08 6 129576132 129578418 2287 - 1.556 1.845 0.982
ENSG00000146376 E002 8.1972797 0.0021943527 1.248003e-01 2.268476e-01 6 129578419 129578482 64 - 0.852 1.036 0.688
ENSG00000146376 E003 16.2472959 0.0011909827 1.094273e-02 3.102511e-02 6 129578483 129578604 122 - 1.106 1.330 0.794
ENSG00000146376 E004 14.6534196 0.0011806568 2.356453e-01 3.691307e-01 6 129580070 129580131 62 - 1.095 1.207 0.404
ENSG00000146376 E005 18.0508299 0.0013010490 4.344412e-01 5.768161e-01 6 129583988 129584112 125 - 1.190 1.258 0.239
ENSG00000146376 E006 0.0000000       6 129596789 129596875 87 -      
ENSG00000146376 E007 0.1515154 0.0432278224 1.000000e+00   6 129598871 129599215 345 - 0.065 0.001 -6.899
ENSG00000146376 E008 17.1581547 0.0010520202 8.256727e-01 8.902491e-01 6 129599216 129599356 141 - 1.195 1.170 -0.086
ENSG00000146376 E009 17.0108491 0.0183717870 2.727459e-01 4.116973e-01 6 129600642 129600848 207 - 1.213 1.084 -0.461
ENSG00000146376 E010 8.2498870 0.0022050015 9.298494e-02 1.797906e-01 6 129605877 129605959 83 - 0.941 0.694 -0.970
ENSG00000146376 E011 13.8422289 0.0013928452 1.155668e-01 2.136584e-01 6 129607893 129608052 160 - 1.132 0.949 -0.670
ENSG00000146376 E012 11.8083871 0.0016007589 5.820009e-01 7.064445e-01 6 129611533 129611610 78 - 1.047 0.980 -0.246
ENSG00000146376 E013 10.6544968 0.0018357379 1.491594e-01 2.605221e-01 6 129616212 129616303 92 - 1.028 0.840 -0.707
ENSG00000146376 E014 18.4018770 0.0109203388 1.608493e-01 2.762284e-01 6 129618687 129618852 166 - 1.249 1.086 -0.580
ENSG00000146376 E015 16.9068258 0.0010726568 2.436212e-02 6.056625e-02 6 129629353 129629522 170 - 1.225 0.980 -0.884
ENSG00000146376 E016 9.5441477 0.0019335266 2.831757e-02 6.863802e-02 6 129634042 129634105 64 - 1.008 0.694 -1.218
ENSG00000146376 E017 18.8582857 0.0032494170 5.982898e-02 1.263226e-01 6 129638394 129638629 236 - 1.257 1.062 -0.696
ENSG00000146376 E018 16.4414153 0.0147399608 1.070737e-01 2.012164e-01 6 129641816 129642018 203 - 1.203 1.011 -0.693
ENSG00000146376 E019 10.3500444 0.0028384292 3.858109e-02 8.841642e-02 6 129710024 129710177 154 - 1.034 0.749 -1.093