ENSG00000146350

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000275159 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding protein_coding 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.49081634 0.00000000 0.97980628 0.00000000 0.23449691 6.629074 0.13505000 0.00000000 0.2196333 0.21963333 3.416273e-04 1.296204e-16 FALSE TRUE
ENST00000368464 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding processed_transcript 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.05108075 0.11211252 0.03887515 0.02185245 0.03887515 -1.321038 0.03097917 0.12626667 0.0083000 -0.11796667 3.081319e-03 1.296204e-16 FALSE TRUE
ENST00000398197 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding processed_transcript 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.30922829 0.43641354 0.00000000 0.12295575 0.00000000 -5.480309 0.24023333 0.47906667 0.0000000 -0.47906667 1.296204e-16 1.296204e-16 FALSE TRUE
ENST00000398212 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding protein_coding 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.61231855 0.04436507 1.31033947 0.04436507 0.10740008 4.602085 0.15235000 0.05386667 0.2984333 0.24456667 4.259597e-02 1.296204e-16 FALSE TRUE
ENST00000509492 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding nonsense_mediated_decay 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.29525920 0.11195558 0.66955064 0.07182429 0.01303093 2.478225 0.08655833 0.10376667 0.1520000 0.04823333 8.588475e-01 1.296204e-16 FALSE FALSE
ENST00000519972 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding retained_intron 2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.28682867 0.00000000 0.00000000 0.00000000 0.00000000 0.000000 0.09663333 0.00000000 0.0000000 0.00000000   1.296204e-16 FALSE FALSE
MSTRG.28894.8 ENSG00000146350 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D32 protein_coding   2.831655 0.8896157 4.419251 0.1732835 0.1569005 2.299682 0.61518651 0.17628383 1.34819072 0.02581527 0.10602725 2.866112 0.19034167 0.22353333 0.3046333 0.08110000 3.532077e-01 1.296204e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146350 E001 22.3491302 0.0417695228 1.262212e-06 1.025924e-05 6 121079494 121080236 743 - 1.072 1.662 2.059
ENSG00000146350 E002 24.3749930 0.0107288298 7.294513e-04 3.004835e-03 6 121080237 121080858 622 - 1.196 1.518 1.120
ENSG00000146350 E003 8.9601434 0.0020459977 5.024152e-02 1.095872e-01 6 121080859 121080890 32 - 0.810 1.069 0.977
ENSG00000146350 E004 17.8089647 0.0010331778 5.124127e-03 1.626674e-02 6 121090853 121091041 189 - 1.075 1.351 0.977
ENSG00000146350 E005 0.3729606 0.0312904639 1.432756e-01 2.524824e-01 6 121095910 121096258 349 - 0.049 0.278 2.919
ENSG00000146350 E006 17.5541083 0.0010667615 9.751755e-03 2.815319e-02 6 121106023 121106163 141 - 1.075 1.334 0.916
ENSG00000146350 E007 0.2924217 0.0275360112 1.000000e+00   6 121106164 121106170 7 - 0.092 0.000 -9.052
ENSG00000146350 E008 3.0406464 0.0054526166 1.031871e-01 1.953286e-01 6 121112386 121112504 119 - 0.444 0.738 1.330
ENSG00000146350 E009 21.8838200 0.0009921873 2.622282e-02 6.440274e-02 6 121112505 121112659 155 - 1.179 1.384 0.721
ENSG00000146350 E010 14.2593596 0.0044956204 1.331742e-01 2.385561e-01 6 121113062 121113177 116 - 1.010 1.184 0.630
ENSG00000146350 E011 8.7687003 0.0160082146 9.118942e-01 9.481045e-01 6 121115172 121115241 70 - 0.847 0.861 0.052
ENSG00000146350 E012 1.6887731 0.0081333665 1.860613e-05 1.162942e-04 6 121115242 121115622 381 - 0.132 0.861 4.139
ENSG00000146350 E013 3.0397040 0.0051802841 8.112144e-01 8.802056e-01 6 121115623 121115747 125 - 0.496 0.445 -0.254
ENSG00000146350 E014 10.6489226 0.0289807409 7.333675e-01 8.245896e-01 6 121126378 121126454 77 - 0.927 0.863 -0.244
ENSG00000146350 E015 6.1919366 0.0086168034 3.652225e-01 5.095615e-01 6 121126455 121126461 7 - 0.740 0.566 -0.745
ENSG00000146350 E016 0.3686942 0.0313151961 1.434407e-01 2.526986e-01 6 121129869 121129924 56 - 0.049 0.278 2.919
ENSG00000146350 E017 11.5141312 0.0311305551 9.843452e-01 9.942909e-01 6 121131627 121131752 126 - 0.956 0.954 -0.006
ENSG00000146350 E018 0.4417471 0.7373127481 1.000000e+00 1.000000e+00 6 121139774 121139836 63 - 0.132 0.000 -9.635
ENSG00000146350 E019 11.4404275 0.0017225321 7.163353e-01 8.120319e-01 6 121160010 121160103 94 - 0.962 0.911 -0.193
ENSG00000146350 E020 13.8400439 0.0012891717 9.474600e-02 1.824918e-01 6 121160948 121161056 109 - 1.049 0.804 -0.925
ENSG00000146350 E021 0.0000000       6 121162970 121163062 93 -      
ENSG00000146350 E022 13.6440808 0.0014453816 3.912396e-01 5.351719e-01 6 121170405 121170527 123 - 1.030 0.911 -0.441
ENSG00000146350 E023 0.5848434 0.0250774232 1.000000e+00 1.000000e+00 6 121192375 121192542 168 - 0.168 0.000 -10.052
ENSG00000146350 E024 10.2799669 0.0175175009 7.377417e-01 8.278275e-01 6 121205075 121205163 89 - 0.916 0.860 -0.211
ENSG00000146350 E025 12.8293731 0.0041793910 3.083313e-01 4.506089e-01 6 121223236 121223352 117 - 1.010 0.861 -0.562
ENSG00000146350 E026 0.4375944 0.0266804158 1.000000e+00 1.000000e+00 6 121224363 121224451 89 - 0.132 0.000 -9.637
ENSG00000146350 E027 0.0000000       6 121236971 121237062 92 -      
ENSG00000146350 E028 11.5419062 0.0017015951 7.534560e-01 8.393893e-01 6 121239070 121239188 119 - 0.957 0.911 -0.171
ENSG00000146350 E029 10.2041057 0.0016798719 4.710439e-01 6.102049e-01 6 121241465 121241552 88 - 0.915 0.804 -0.429
ENSG00000146350 E030 11.2595314 0.0016471360 5.785108e-02 1.229453e-01 6 121242201 121242339 139 - 0.974 0.661 -1.234
ENSG00000146350 E031 9.3803256 0.0021797610 6.133100e-01 7.320279e-01 6 121255328 121255410 83 - 0.883 0.804 -0.306
ENSG00000146350 E032 13.0589212 0.0014136080 9.891248e-01 9.972630e-01 6 121256084 121256285 202 - 1.000 0.997 -0.010
ENSG00000146350 E033 11.7134689 0.0014975172 4.636990e-01 6.034703e-01 6 121279121 121279245 125 - 0.968 0.861 -0.405
ENSG00000146350 E034 12.9927417 0.0014337564 1.341504e-01 2.399209e-01 6 121281544 121281686 143 - 1.025 0.804 -0.839
ENSG00000146350 E035 10.7673076 0.0015499180 7.390702e-02 1.497579e-01 6 121283818 121283910 93 - 0.957 0.660 -1.171
ENSG00000146350 E036 15.0669160 0.0012602663 5.230674e-02 1.132666e-01 6 121292053 121292193 141 - 1.084 0.804 -1.053
ENSG00000146350 E037 12.6362805 0.0015270987 7.314068e-02 1.485419e-01 6 121294570 121294660 91 - 1.015 0.738 -1.066
ENSG00000146350 E038 9.4321482 0.0028563265 5.591378e-02 1.195429e-01 6 121299446 121299505 60 - 0.909 0.566 -1.406
ENSG00000146350 E039 13.2655822 0.0013893309 1.255943e-01 2.279962e-01 6 121303617 121303761 145 - 1.030 0.804 -0.856
ENSG00000146350 E040 7.2029992 0.0034707897 6.664584e-02 1.378068e-01 6 121304365 121304426 62 - 0.809 0.445 -1.607
ENSG00000146350 E041 10.7052792 0.0021015245 7.368688e-04 3.032094e-03 6 121304522 121304625 104 - 0.974 0.277 -3.233
ENSG00000146350 E042 10.2042115 0.0016229834 6.124485e-05 3.376005e-04 6 121304755 121304833 79 - 0.963 0.000 -14.160
ENSG00000146350 E043 9.7793025 0.0018202105 9.169410e-05 4.829484e-04 6 121307976 121308024 49 - 0.945 0.000 -14.096
ENSG00000146350 E044 8.7208847 0.0019025199 2.328323e-04 1.103396e-03 6 121308025 121308101 77 - 0.902 0.000 -13.935
ENSG00000146350 E045 8.3187898 0.0019449656 2.919146e-02 7.037085e-02 6 121310779 121310847 69 - 0.869 0.445 -1.839
ENSG00000146350 E046 16.9205486 0.0010762724 1.879749e-02 4.889174e-02 6 121317495 121317672 178 - 1.136 0.804 -1.240
ENSG00000146350 E047 0.3268771 0.0318959187 1.430797e-01   6 121317673 121317690 18 - 0.049 0.277 2.917
ENSG00000146350 E048 15.4844486 0.0012300633 2.009268e-01 3.273393e-01 6 121321633 121321794 162 - 1.084 0.911 -0.638
ENSG00000146350 E049 12.2420950 0.0015730671 5.409676e-01 6.720176e-01 6 121334276 121334431 156 - 0.957 1.035 0.289
ENSG00000146350 E050 2.5153271 0.0059546284 9.179704e-01 9.521571e-01 6 121334432 121334480 49 - 0.425 0.446 0.110
ENSG00000146350 E051 4.4844333 0.0039385243 7.288741e-01 8.212214e-01 6 121334618 121334745 128 - 0.598 0.661 0.273