ENSG00000146021

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309755 ENSG00000146021 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL3 protein_coding protein_coding 0.6308932 0.2415578 0.6757383 0.02042673 0.0180204 1.446768 0.217507691 0.1482203 0.15139829 0.07514681 0.15139829 0.02869083 0.38870000 0.6463333 0.21600000 -0.43033333 0.43153238 0.03124414 FALSE TRUE
ENST00000506491 ENSG00000146021 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL3 protein_coding protein_coding 0.6308932 0.2415578 0.6757383 0.02042673 0.0180204 1.446768 0.200126068 0.0880570 0.11661809 0.08805700 0.06791748 0.36879102 0.30739583 0.3333333 0.17400000 -0.15933333 0.95891497 0.03124414 FALSE TRUE
ENST00000508657 ENSG00000146021 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL3 protein_coding protein_coding 0.6308932 0.2415578 0.6757383 0.02042673 0.0180204 1.446768 0.092734505 0.0000000 0.27936333 0.00000000 0.09339946 4.85481021 0.13899167 0.0000000 0.41980000 0.41980000 0.03124414 0.03124414 FALSE TRUE
ENST00000509694 ENSG00000146021 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL3 protein_coding retained_intron 0.6308932 0.2415578 0.6757383 0.02042673 0.0180204 1.446768 0.006707606 0.0000000 0.03428861 0.00000000 0.01836975 2.14693574 0.01018333 0.0000000 0.05136667 0.05136667 0.71234724 0.03124414   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000146021 E001 3.5268596 0.1139459895 0.032440589 0.076705178 5 137617500 137617658 159 - 0.467 0.842 1.622
ENSG00000146021 E002 6.6519060 0.0958562358 0.179179259 0.300116467 5 137617659 137617864 206 - 0.774 0.981 0.798
ENSG00000146021 E003 39.7804520 0.0009262827 0.002314754 0.008197012 5 137617865 137621220 3356 - 1.544 1.670 0.427
ENSG00000146021 E004 5.5119517 0.0033708618 0.337161945 0.481024784 5 137621221 137621501 281 - 0.833 0.693 -0.562
ENSG00000146021 E005 5.5685452 0.0331239552 0.187389313 0.310447944 5 137621502 137621938 437 - 0.860 0.648 -0.861
ENSG00000146021 E006 2.2207153 0.0070591389 0.169964199 0.288159294 5 137621939 137622126 188 - 0.554 0.298 -1.391
ENSG00000146021 E007 3.4332740 0.0061219721 0.888454742 0.932676501 5 137625753 137625896 144 - 0.626 0.597 -0.128
ENSG00000146021 E008 0.2924217 0.0290785164 0.552100639   5 137628059 137628296 238 - 0.155 0.000 -13.049
ENSG00000146021 E009 2.8442779 0.0067863646 0.638922691 0.752452215 5 137628297 137628437 141 - 0.527 0.597 0.320
ENSG00000146021 E010 0.8899506 0.0144048543 0.077437331 0.155347806 5 137628438 137629943 1506 - 0.360 0.000 -14.249
ENSG00000146021 E011 0.8825757 0.0151002869 0.456190445 0.596644192 5 137634037 137634043 7 - 0.317 0.174 -1.131
ENSG00000146021 E012 2.2456083 0.0072438620 0.025253554 0.062394331 5 137634044 137634165 122 - 0.604 0.174 -2.615
ENSG00000146021 E013 1.8433865 0.0114484776 0.702707423 0.801513689 5 137637294 137637395 102 - 0.468 0.394 -0.393
ENSG00000146021 E014 3.3592061 0.0052876956 0.202586814 0.329440288 5 137638953 137639150 198 - 0.688 0.472 -0.978
ENSG00000146021 E015 2.1100245 0.2458026227 0.933849381 0.962305005 5 137639860 137639977 118 - 0.499 0.464 -0.175
ENSG00000146021 E016 2.8315078 0.0264964682 0.395818299 0.539602606 5 137658131 137658280 150 - 0.626 0.471 -0.720
ENSG00000146021 E017 0.0000000       5 137660711 137661914 1204 -      
ENSG00000146021 E018 1.8069817 0.0079517470 0.287544147 0.428049032 5 137661915 137662031 117 - 0.499 0.297 -1.130
ENSG00000146021 E019 0.1515154 0.0440157116 1.000000000   5 137671767 137671952 186 - 0.085 0.000 -12.190
ENSG00000146021 E020 0.0000000       5 137672667 137672753 87 -      
ENSG00000146021 E021 0.1472490 0.0433287468 1.000000000   5 137672754 137672858 105 - 0.085 0.000 -12.191
ENSG00000146021 E022 0.1472490 0.0433287468 1.000000000   5 137672859 137672927 69 - 0.085 0.000 -12.191
ENSG00000146021 E023 0.0000000       5 137673675 137673897 223 -      
ENSG00000146021 E024 0.2214452 0.0383006313 0.205195953   5 137677417 137677469 53 - 0.000 0.174 13.026
ENSG00000146021 E025 1.4329743 0.0098799034 0.829315462 0.892830178 5 137677545 137677649 105 - 0.360 0.394 0.193
ENSG00000146021 E026 0.4772466 0.0212366771 0.193833943 0.318599649 5 137677650 137677654 5 - 0.085 0.297 2.191
ENSG00000146021 E027 2.0660027 0.0073297426 0.517086817 0.651541357 5 137692285 137692447 163 - 0.435 0.539 0.514
ENSG00000146021 E028 0.1482932 0.0417221650 0.207059966   5 137692448 137692685 238 - 0.000 0.173 13.016
ENSG00000146021 E029 3.1022719 0.0057460556 0.310174508 0.452546445 5 137698287 137698408 122 - 0.648 0.472 -0.807
ENSG00000146021 E030 0.4375944 0.0263195698 0.316750433 0.459548774 5 137705992 137706049 58 - 0.216 0.000 -13.513
ENSG00000146021 E031 0.4375944 0.0263195698 0.316750433 0.459548774 5 137706050 137706147 98 - 0.216 0.000 -13.513
ENSG00000146021 E032 0.0000000       5 137706148 137706436 289 -      
ENSG00000146021 E033 0.0000000       5 137707549 137707609 61 -      
ENSG00000146021 E034 2.2551852 0.0066966877 0.128668260 0.232301070 5 137709750 137709856 107 - 0.580 0.297 -1.509
ENSG00000146021 E035 2.1058599 0.0715318083 0.217520205 0.347393824 5 137720465 137720584 120 - 0.554 0.297 -1.397
ENSG00000146021 E036 0.5891098 0.0187414901 0.191428996 0.315542020 5 137720678 137721310 633 - 0.270 0.000 -13.831
ENSG00000146021 E037 0.1515154 0.0440157116 1.000000000   5 137735633 137735742 110 - 0.085 0.000 -12.190
ENSG00000146021 E038 0.0000000       5 137735743 137735817 75 -      
ENSG00000146021 E039 0.0000000       5 137735818 137736089 272 -