ENSG00000145996

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000274695 ENSG00000145996 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKAL1 protein_coding protein_coding 9.957553 7.601707 12.97469 0.6132846 0.2609818 0.7705199 5.8049661 3.953201 7.52802737 0.4705167 0.22408868 0.9275208 0.60060417 0.5209667 0.5810333 0.06006667 7.106291e-01 4.647124e-30 FALSE TRUE
ENST00000476517 ENSG00000145996 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKAL1 protein_coding processed_transcript 9.957553 7.601707 12.97469 0.6132846 0.2609818 0.7705199 0.5811318 2.303465 0.03639837 0.4901510 0.03639837 -5.6398374 0.08639167 0.2985000 0.0027000 -0.29580000 9.466175e-13 4.647124e-30 FALSE FALSE
MSTRG.27720.1 ENSG00000145996 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKAL1 protein_coding   9.957553 7.601707 12.97469 0.6132846 0.2609818 0.7705199 1.0299124 1.137061 0.99190740 0.1533944 0.23345762 -0.1951924 0.11317083 0.1533000 0.0765000 -0.07680000 1.989429e-01 4.647124e-30 FALSE TRUE
MSTRG.27720.3 ENSG00000145996 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKAL1 protein_coding   9.957553 7.601707 12.97469 0.6132846 0.2609818 0.7705199 2.1645299 0.000000 3.98717982 0.0000000 0.35123491 8.6428387 0.15848750 0.0000000 0.3065333 0.30653333 4.647124e-30 4.647124e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145996 E001 0.2955422 0.0288724730 7.155283e-01   6 20534439 20534456 18 + 0.097 0.153 0.755
ENSG00000145996 E002 0.8845503 0.1622237705 6.668433e-01 7.742417e-01 6 20534457 20534468 12 + 0.243 0.350 0.724
ENSG00000145996 E003 8.2971725 0.0024289764 2.180059e-01 3.480199e-01 6 20534469 20534480 12 + 1.022 0.890 -0.491
ENSG00000145996 E004 37.3638960 0.0005919311 3.703812e-02 8.554627e-02 6 20534481 20534574 94 + 1.626 1.515 -0.381
ENSG00000145996 E005 3.5064406 0.0044511782 1.389862e-01 2.467022e-01 6 20535087 20535199 113 + 0.721 0.494 -1.007
ENSG00000145996 E006 43.1405518 0.0005095869 2.696594e-03 9.363642e-03 6 20535351 20535394 44 + 1.702 1.547 -0.525
ENSG00000145996 E007 0.5169874 0.0204854884 3.155128e-01 4.582861e-01 6 20546341 20546345 5 + 0.097 0.266 1.758
ENSG00000145996 E008 79.4227076 0.0003880791 3.164643e-05 1.872543e-04 6 20546346 20546523 178 + 1.964 1.806 -0.532
ENSG00000145996 E009 62.0050133 0.0004440604 2.028291e-03 7.314659e-03 6 20548593 20548705 113 + 1.846 1.714 -0.444
ENSG00000145996 E010 6.6666191 0.0029388455 1.132644e-01 2.102820e-01 6 20558542 20559672 1131 + 0.796 0.995 0.756
ENSG00000145996 E011 47.0914883 0.0052437142 1.083034e-02 3.075939e-02 6 20649293 20649377 85 + 1.733 1.579 -0.524
ENSG00000145996 E012 56.4577114 0.0004947615 1.215527e-03 4.693228e-03 6 20739519 20739615 97 + 1.808 1.661 -0.497
ENSG00000145996 E013 47.4625872 0.0006509449 1.048327e-02 2.993886e-02 6 20758595 20758643 49 + 1.727 1.601 -0.426
ENSG00000145996 E014 63.0997012 0.0003641011 7.291711e-05 3.941881e-04 6 20781145 20781265 121 + 1.867 1.696 -0.577
ENSG00000145996 E015 53.5945173 0.0004680441 2.178813e-02 5.523424e-02 6 20846075 20846178 104 + 1.775 1.673 -0.345
ENSG00000145996 E016 69.3235141 0.0005479511 5.817732e-03 1.813257e-02 6 20955419 20955585 167 + 1.884 1.774 -0.372
ENSG00000145996 E017 64.1219910 0.0004230875 5.431490e-01 6.737863e-01 6 21000227 21000372 146 + 1.815 1.798 -0.058
ENSG00000145996 E018 0.2966881 0.0272369440 3.816821e-01   6 21047110 21047152 43 + 0.176 0.001 -8.735
ENSG00000145996 E019 74.0580338 0.0004460789 7.691296e-02 1.545608e-01 6 21065048 21065228 181 + 1.895 1.832 -0.215
ENSG00000145996 E020 43.2589040 0.0005573383 1.464901e-02 3.970511e-02 6 21108401 21108463 63 + 1.691 1.568 -0.419
ENSG00000145996 E021 53.1335510 0.0004860969 9.175707e-01 9.519186e-01 6 21198021 21198104 84 + 1.727 1.731 0.016
ENSG00000145996 E022 1.2190178 0.0110433590 1.155419e-01 2.136249e-01 6 21200812 21200892 81 + 0.440 0.153 -2.050
ENSG00000145996 E023 77.7935193 0.0025510981 4.995907e-01 6.358778e-01 6 21201110 21201274 165 + 1.877 1.918 0.137
ENSG00000145996 E024 316.2411673 0.0005151037 1.499657e-51 9.933758e-49 6 21230848 21232404 1557 + 2.352 2.646 0.981