ENSG00000145431

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000274071 ENSG00000145431 HEK293_OSMI2_2hA HEK293_TMG_2hB PDGFC protein_coding nonsense_mediated_decay 2.661751 1.362435 3.860919 0.159701 0.2618414 1.495938 1.78946365 1.22229242 2.2280569 0.13497316 0.2365696 0.8609021 0.72210000 0.89890000 0.58620000 -0.3127000000 0.003420329 0.003420329 FALSE TRUE
ENST00000422544 ENSG00000145431 HEK293_OSMI2_2hA HEK293_TMG_2hB PDGFC protein_coding protein_coding 2.661751 1.362435 3.860919 0.159701 0.2618414 1.495938 0.06375352 0.00000000 0.2427687 0.00000000 0.1322827 4.6597461 0.01684583 0.00000000 0.06286667 0.0628666667 0.397533325 0.003420329 FALSE TRUE
ENST00000504672 ENSG00000145431 HEK293_OSMI2_2hA HEK293_TMG_2hB PDGFC protein_coding processed_transcript 2.661751 1.362435 3.860919 0.159701 0.2618414 1.495938 0.22209712 0.00000000 0.5331917 0.00000000 0.2784229 5.7633895 0.06661667 0.00000000 0.12910000 0.1291000000 0.332261187 0.003420329 FALSE FALSE
MSTRG.25644.4 ENSG00000145431 HEK293_OSMI2_2hA HEK293_TMG_2hB PDGFC protein_coding   2.661751 1.362435 3.860919 0.159701 0.2618414 1.495938 0.21554914 0.04915801 0.1142489 0.04915801 0.1142489 1.0705871 0.06657083 0.02956667 0.02870000 -0.0008666667 0.874477400 0.003420329 FALSE TRUE
MSTRG.25644.6 ENSG00000145431 HEK293_OSMI2_2hA HEK293_TMG_2hB PDGFC protein_coding   2.661751 1.362435 3.860919 0.159701 0.2618414 1.495938 0.20853274 0.00000000 0.3522145 0.00000000 0.1873728 5.1787725 0.05113750 0.00000000 0.09620000 0.0962000000 0.386168066 0.003420329 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145431 E001 1.1124400 0.0116938603 8.004485e-01 8.727981e-01 4 156760454 156761613 1160 - 0.331 0.285 -0.300
ENSG00000145431 E002 71.5919452 0.0005525990 2.518543e-10 4.144636e-09 4 156761614 156762802 1189 - 1.745 1.984 0.806
ENSG00000145431 E003 9.3698701 0.0031878951 8.227653e-01 8.882526e-01 4 156762803 156762822 20 - 1.015 0.991 -0.089
ENSG00000145431 E004 21.2863259 0.0009976777 9.543690e-01 9.754118e-01 4 156762823 156762947 125 - 1.343 1.340 -0.011
ENSG00000145431 E005 20.3904219 0.0009578313 3.485115e-01 4.927419e-01 4 156762948 156763089 142 - 1.295 1.366 0.248
ENSG00000145431 E006 20.3295861 0.0050289342 2.216490e-01 3.523667e-01 4 156763090 156763206 117 - 1.356 1.258 -0.345
ENSG00000145431 E007 12.7734794 0.0030583728 3.751717e-02 8.643563e-02 4 156767773 156767781 9 - 1.200 0.991 -0.755
ENSG00000145431 E008 18.2910872 0.0011607989 5.457227e-01 6.760186e-01 4 156767782 156767876 95 - 1.295 1.248 -0.167
ENSG00000145431 E009 18.1093267 0.0012483189 6.389253e-01 7.524522e-01 4 156767877 156767961 85 - 1.253 1.291 0.134
ENSG00000145431 E010 14.0955497 0.0113553963 4.130439e-01 5.565808e-01 4 156767962 156767990 29 - 1.132 1.213 0.286
ENSG00000145431 E011 0.9244060 0.0136968632 3.763850e-01 5.207123e-01 4 156770182 156770643 462 - 0.331 0.165 -1.300
ENSG00000145431 E012 22.0423078 0.0009173497 4.992603e-02 1.090411e-01 4 156772686 156772788 103 - 1.398 1.248 -0.522
ENSG00000145431 E013 20.9270626 0.0010130199 1.714229e-01 2.900457e-01 4 156772789 156772893 105 - 1.365 1.259 -0.369
ENSG00000145431 E014 0.1515154 0.0433922404 1.000000e+00   4 156773677 156773717 41 - 0.089 0.000 -8.975
ENSG00000145431 E015 0.1472490 0.0441470002 1.000000e+00   4 156774372 156774478 107 - 0.089 0.000 -8.973
ENSG00000145431 E016 0.0000000       4 156778201 156778309 109 -      
ENSG00000145431 E017 0.0000000       4 156779088 156779158 71 -      
ENSG00000145431 E018 12.4505096 0.0075769433 8.338518e-01 8.959472e-01 4 156810837 156810868 32 - 1.125 1.100 -0.090
ENSG00000145431 E019 12.5644455 0.0015002705 6.482131e-01 7.597669e-01 4 156810869 156810952 84 - 1.102 1.146 0.157
ENSG00000145431 E020 12.0669256 0.0014386314 8.933731e-01 9.358495e-01 4 156810953 156811017 65 - 1.102 1.116 0.049
ENSG00000145431 E021 0.0000000       4 156835915 156836007 93 -      
ENSG00000145431 E022 20.9857533 0.0011509602 7.037976e-02 1.439812e-01 4 156850221 156850416 196 - 1.377 1.236 -0.493
ENSG00000145431 E023 4.9102581 0.0050380892 9.616308e-02 1.846169e-01 4 156861429 156861489 61 - 0.827 0.578 -1.035
ENSG00000145431 E024 0.0000000       4 156873906 156873972 67 -      
ENSG00000145431 E025 0.7437457 0.0156455152 5.272043e-01 6.603305e-01 4 156967329 156967745 417 - 0.282 0.165 -0.980
ENSG00000145431 E026 31.4032854 0.0006581506 5.057240e-03 1.608757e-02 4 156970786 156971799 1014 - 1.556 1.375 -0.621