ENSG00000145428

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000347063 ENSG00000145428 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF175 protein_coding protein_coding 0.2639179 0.4229018 0.2330264 0.04940114 0.1020834 -0.8329268 0.173838934 0.3063568 0.00000000 0.04182954 0.00000000 -4.9834806 0.67837500 0.7408333 0.0000000 -0.7408333 0.00789746 0.00789746 FALSE TRUE
ENST00000503694 ENSG00000145428 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF175 protein_coding nonsense_mediated_decay 0.2639179 0.4229018 0.2330264 0.04940114 0.1020834 -0.8329268 0.008563596 0.0000000 0.01595170 0.00000000 0.01030654 1.3758288 0.05696667 0.0000000 0.1325667 0.1325667 0.69421370 0.00789746 TRUE TRUE
ENST00000508967 ENSG00000145428 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF175 protein_coding processed_transcript 0.2639179 0.4229018 0.2330264 0.04940114 0.1020834 -0.8329268 0.005764137 0.0000000 0.04611309 0.00000000 0.04611309 2.4883374 0.03818333 0.0000000 0.3054667 0.3054667 0.84674919 0.00789746 FALSE FALSE
ENST00000509321 ENSG00000145428 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF175 protein_coding retained_intron 0.2639179 0.4229018 0.2330264 0.04940114 0.1020834 -0.8329268 0.008038851 0.0000000 0.06431080 0.00000000 0.06431080 2.8935720 0.01843750 0.0000000 0.1475000 0.1475000 0.50470926 0.00789746 FALSE TRUE
ENST00000513656 ENSG00000145428 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF175 protein_coding nonsense_mediated_decay 0.2639179 0.4229018 0.2330264 0.04940114 0.1020834 -0.8329268 0.053093098 0.1165451 0.10665082 0.06283771 0.07171334 -0.1174548 0.18077083 0.2591667 0.4144667 0.1553000 0.78368645 0.00789746 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145428 E001 3.7535362 0.005746056 0.006815379 0.02074308 4 153710160 153710489 330 - 0.000 0.636 12.718
ENSG00000145428 E002 1.3577419 0.133566910 0.233093408 0.36608978 4 153712475 153712482 8 - 0.000 0.328 13.686
ENSG00000145428 E003 2.1338522 0.213829997 0.449927291 0.59112199 4 153712483 153712576 94 - 0.223 0.424 1.303
ENSG00000145428 E004 0.2924217 0.048603992 0.013534769   4 153713083 153715528 2446 - 0.369 0.000 -15.536
ENSG00000145428 E005 3.3631356 0.007387341 0.654286169 0.76450212 4 153715529 153715662 134 - 0.566 0.528 -0.174
ENSG00000145428 E006 3.1813571 0.005905474 0.653141871 0.76370204 4 153720184 153720269 86 - 0.566 0.528 -0.177
ENSG00000145428 E007 1.7056101 0.011202296 0.530137381 0.66279733 4 153720270 153720304 35 - 0.223 0.384 1.086
ENSG00000145428 E008 1.4059033 0.012047832 0.568930596 0.69554401 4 153723351 153723399 49 - 0.369 0.290 -0.496
ENSG00000145428 E009 0.9630129 0.015567375 0.326834174 0.47027809 4 153723400 153723458 59 - 0.369 0.213 -1.084
ENSG00000145428 E010 1.0266130 0.015196691 1.000000000 1.00000000 4 153728207 153728361 155 - 0.223 0.253 0.240
ENSG00000145428 E011 0.0000000       4 153745669 153745727 59 -      
ENSG00000145428 E012 0.0000000       4 153748405 153748535 131 -      
ENSG00000145428 E013 0.1515154 0.046161768 0.081217530   4 153748536 153748644 109 - 0.223 0.000 -14.736
ENSG00000145428 E014 0.3030308 0.094568196 0.016888028   4 153748645 153748749 105 - 0.369 0.000 -15.510
ENSG00000145428 E015 0.2987644 0.029078516 0.013431380   4 153748750 153748786 37 - 0.369 0.000 -15.549
ENSG00000145428 E016 0.0000000       4 153748787 153748827 41 -      
ENSG00000145428 E017 0.8803962 0.023685651 0.900333122 0.94040716 4 153751438 153751447 10 - 0.223 0.213 -0.080
ENSG00000145428 E018 1.1802048 0.012198908 0.455295385 0.59582731 4 153751448 153751475 28 - 0.369 0.253 -0.760
ENSG00000145428 E019 1.8465295 0.008552309 0.940420152 0.96659722 4 153759797 153759873 77 - 0.369 0.384 0.087
ENSG00000145428 E020 0.5180316 0.021768165 0.846467405 0.90459238 4 153759874 153759878 5 - 0.000 0.169 12.655
ENSG00000145428 E021 1.0360631 0.014217446 0.301677906 0.44348893 4 153759879 153759961 83 - 0.000 0.290 13.503
ENSG00000145428 E022 0.6663248 0.019828489 0.558845540 0.68703187 4 153759962 153759970 9 - 0.000 0.213 12.999
ENSG00000145428 E023 0.5180316 0.021768165 0.846467405 0.90459238 4 153759971 153759997 27 - 0.000 0.169 12.655
ENSG00000145428 E024 0.1482932 0.046169562 1.000000000   4 153759998 153760007 10 - 0.000 0.064 11.348
ENSG00000145428 E025 0.1482932 0.046169562 1.000000000   4 153760008 153760010 3 - 0.000 0.064 11.348
ENSG00000145428 E026 0.1482932 0.046169562 1.000000000   4 153760011 153760024 14 - 0.000 0.064 11.348